Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0990.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02960.1 68418.m00239 40S ribosomal protein S23 (RPS23B) ribo... 121 3e-28
At3g09680.1 68416.m01147 40S ribosomal protein S23 (RPS23A) simi... 113 5e-26
At5g50790.1 68418.m06292 nodulin MtN3 family protein similar to ... 29 2.3
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 29 2.3
At1g32960.1 68414.m04059 subtilase family protein contains simil... 28 4.0
At1g32940.1 68414.m04057 subtilase family protein contains simil... 28 4.0
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha... 27 9.3
At4g10550.1 68417.m01727 subtilase family protein contains simil... 27 9.3
>At5g02960.1 68418.m00239 40S ribosomal protein S23 (RPS23B)
ribosomal protein S23, Fragaria x ananassa, PIR:S56673
Length = 142
Score = 121 bits (291), Expect = 3e-28
Identities = 55/79 (69%), Positives = 64/79 (81%)
Frame = +1
Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180
GK RG+ RK R QRWADK++KK+H+G +WK PF G+SHAKGIVLEK+G+EAKQ
Sbjct: 2 GKTRGMGAGRKLKRLRINQRWADKQYKKSHLGNEWK-KPFAGSSHAKGIVLEKIGIEAKQ 60
Query: 181 PNSAIRKCVRVQLIKNGKK 237
PNSAIRKC RVQLIKNGKK
Sbjct: 61 PNSAIRKCARVQLIKNGKK 79
Score = 113 bits (273), Expect = 5e-26
Identities = 51/58 (87%), Positives = 56/58 (96%)
Frame = +3
Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS 425
P DGCLN+IEENDEVL+AGFGRKGHAVGDIPGVRFKVVKV+ VSLLAL+KEKKE+PRS
Sbjct: 85 PNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS 142
>At3g09680.1 68416.m01147 40S ribosomal protein S23 (RPS23A) similar
to 40S ribosomal protein S23 (S12) GB:P46297 from
[Fragaria x ananassa]
Length = 142
Score = 113 bits (273), Expect = 5e-26
Identities = 51/58 (87%), Positives = 56/58 (96%)
Frame = +3
Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS 425
P DGCLN+IEENDEVL+AGFGRKGHAVGDIPGVRFKVVKV+ VSLLAL+KEKKE+PRS
Sbjct: 85 PNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS 142
Score = 112 bits (269), Expect = 1e-25
Identities = 53/79 (67%), Positives = 61/79 (77%)
Frame = +1
Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180
GK RG+ RK R QRWADK +KK++ G +WK PF +SHAKGIVLEK+G+EAKQ
Sbjct: 2 GKTRGMGAGRKLKQLRITQRWADKHYKKSNRGNEWK-KPFACSSHAKGIVLEKIGIEAKQ 60
Query: 181 PNSAIRKCVRVQLIKNGKK 237
PNSAIRKC RVQLIKNGKK
Sbjct: 61 PNSAIRKCARVQLIKNGKK 79
>At5g50790.1 68418.m06292 nodulin MtN3 family protein similar to
MtN3 GI:1619602 (root nodule development) from [Medicago
truncatula]
Length = 289
Score = 28.7 bits (61), Expect = 2.3
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +3
Query: 360 VVKVANVSLLALYKEKKERPRS*VYIVISDLLSGSALFI 476
VV++ +SL Y KKE+ + +++ D+L A+F+
Sbjct: 80 VVQIVYISLFFFYAPKKEKTLTVKFVLFVDVLGFGAIFV 118
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 28.7 bits (61), Expect = 2.3
Identities = 28/77 (36%), Positives = 35/77 (45%)
Frame = -1
Query: 457 DSRSLITMYTYDLGRSFFSL*RARRDTLATFTTLKRTPGMSPTA*PLRPNPATSTSSFSS 278
DSRS +Y RS R R + + + R+ SP A LR +PA STS
Sbjct: 198 DSRSPSRGRSYSKSRS-----RGRSPSRSRSRSRSRSKSRSPKAKSLRRSPAKSTSRSP- 251
Query: 277 MWFRQPSRGRMRSLSFR 227
R SR + RSLS R
Sbjct: 252 ---RSRSRSKSRSLSPR 265
>At1g32960.1 68414.m04059 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 [Oryza sativa]
Length = 777
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ F+ A + T W+ +PFG A+G
Sbjct: 575 WSPAAFRSAIVTTAWRTDPFGEQIFAEG 602
>At1g32940.1 68414.m04057 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 774
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ F+ A + T W+ +PFG A+G
Sbjct: 572 WSPAAFRSAIVTTAWRTDPFGEQIFAEG 599
>At4g36080.1 68417.m05136 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF00454: Phosphatidylinositol 3-
and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
Length = 3839
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = -2
Query: 495 ILHFMNL*TTRFPTAGHLSQCTPMILVAPFSLCRERGETRWLLLPP 358
+LHF+NL ++ HL Q M+++ + + G+T W ++ P
Sbjct: 1731 VLHFLNLFQSKQLGHDHLVQAMQMLILPMLAHAFQNGQT-WEVIDP 1775
>At4g10550.1 68417.m01727 subtilase family protein contains
similarity to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana]
Length = 778
Score = 26.6 bits (56), Expect = 9.3
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ + A + T WK +PFG A+G
Sbjct: 576 WSPAAIRSAIVTTAWKTDPFGEQIFAEG 603
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,479,095
Number of Sequences: 28952
Number of extensions: 243455
Number of successful extensions: 597
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 595
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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