BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0980.Seq (359 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 2.0 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 2.7 SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 24 8.2 SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyce... 24 8.2 SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 24 8.2 SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 24 8.2 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.8 bits (54), Expect = 2.0 Identities = 13/35 (37%), Positives = 16/35 (45%) Frame = +1 Query: 4 ALMDTVPTVVMEVYHPPAVPVTLMQAQTLQDPVHP 108 A+ P V E YHPP T + +Q P HP Sbjct: 922 AIASPPPPKVGETYHPPTASGT--RVPPVQQPSHP 954 Score = 25.0 bits (52), Expect = 3.5 Identities = 20/58 (34%), Positives = 23/58 (39%) Frame = +1 Query: 49 PPAVPVTLMQAQTLQDPVHPAVAALMDTVPTVVMEVYHPPAVPVTLMQAQTWQDPVHP 222 PP P M A PA+A+ P V E YHPP T + Q P HP Sbjct: 908 PPPPPTASMTASA------PAIAS---PPPPKVGETYHPPTASGT--RVPPVQQPSHP 954 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.4 bits (53), Expect = 2.7 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +1 Query: 76 QAQTLQDPVHPAVAALMDTVPTVVMEVYHP 165 QA + V PAV+A + T P VV V HP Sbjct: 1284 QASAVSSNV-PAVSASISTPPAVVPTVQHP 1312 >SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 180 Score = 23.8 bits (49), Expect = 8.2 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -1 Query: 272 IHFHHHVGTVSISAATAGCTGS 207 IH + + TV I ++T CTGS Sbjct: 130 IHLTYLIDTVVIESSTFWCTGS 151 >SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyces pombe|chr 3|||Manual Length = 363 Score = 23.8 bits (49), Expect = 8.2 Identities = 8/16 (50%), Positives = 10/16 (62%) Frame = -1 Query: 239 ISAATAGCTGSCQVCA 192 +S T GCT C+ CA Sbjct: 62 LSIKTGGCTEDCKYCA 77 Score = 23.8 bits (49), Expect = 8.2 Identities = 8/12 (66%), Positives = 8/12 (66%) Frame = -1 Query: 113 TAGCTGSCKVCA 78 T GCT CK CA Sbjct: 66 TGGCTEDCKYCA 77 >SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 516 Score = 23.8 bits (49), Expect = 8.2 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +1 Query: 103 HPAVAALMDTVPTVVMEVYHPPAVPV 180 HPAV+A T P+ +EVY + V Sbjct: 254 HPAVSAFYVTCPSKGIEVYGQDQIAV 279 >SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 23.8 bits (49), Expect = 8.2 Identities = 7/24 (29%), Positives = 13/24 (54%), Gaps = 2/24 (8%) Frame = -3 Query: 195 CLHQCYWNC--RWMIHFHHDCRNC 130 C H+ + C +W++ + H C C Sbjct: 339 CKHEFHRPCIAKWIVDYRHACPTC 362 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,211,218 Number of Sequences: 5004 Number of extensions: 21921 Number of successful extensions: 60 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 47 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 59 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 110009772 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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