BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0977.Seq (568 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 32 0.31 At3g16940.1 68416.m02165 calmodulin-binding protein similar to a... 29 2.2 At5g28623.1 68418.m03496 hypothetical protein 27 6.6 At3g51330.1 68416.m05619 aspartyl protease family protein contai... 27 6.6 At1g23980.1 68414.m03028 zinc finger (C3HC4-type RING finger) fa... 27 8.7 >At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin family protein similar to arabinogalactan protein [Daucus carota] GI:11322245; contains Pfam profile PF01190: Pollen proteins Ole e I family Length = 359 Score = 31.9 bits (69), Expect = 0.31 Identities = 15/50 (30%), Positives = 20/50 (40%) Frame = +1 Query: 208 LTTYYCFSGGNFQRFHKILFYDLLPSLVPPPRQFVMSSHQPTTPRRQHPH 357 L +CF+ F ++ PSL P P + H P P HPH Sbjct: 12 LVALWCFTSSVFTE--EVNHKTQTPSLAPAPAPYHHGHHHPHPPHHHHPH 59 >At3g16940.1 68416.m02165 calmodulin-binding protein similar to anther ethylene-upregulated protein ER1 GI:11612392 from [Nicotiana tabacum]; contains Pfam profile: PF00612 IQ calmodulin-binding motif (3 copies) Length = 852 Score = 29.1 bits (62), Expect = 2.2 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +2 Query: 374 SLNSP*SCGAQNFPSKASIQQIPTIVENY 460 S++SP S Q FP++ + + I T+V N+ Sbjct: 155 SISSPISVSEQTFPNRVAAEDIDTVVRNH 183 >At5g28623.1 68418.m03496 hypothetical protein Length = 236 Score = 27.5 bits (58), Expect = 6.6 Identities = 13/47 (27%), Positives = 22/47 (46%) Frame = +2 Query: 170 IFETVRPFYCFLNLLHTTVFQEVIFSVSTKYFFTIYYHLWYHPRANL 310 + ET+ F+ + L T FS T YFF+ + + +P+ L Sbjct: 61 LLETIDEFFTKMGLGAFTSMDHATFSEPTNYFFSTMVYTFKNPKTPL 107 >At3g51330.1 68416.m05619 aspartyl protease family protein contains Eukaryotic and viral aspartyl proteases active site, PROSITE:PS00141 Length = 529 Score = 27.5 bits (58), Expect = 6.6 Identities = 14/40 (35%), Positives = 18/40 (45%) Frame = +1 Query: 277 LPSLVPPPRQFVMSSHQPTTPRRQHPHGCQADLIELTMKL 396 LPSL+PPP P R G A+L+ L +L Sbjct: 480 LPSLLPPPAAATPPQIDPRNSTRNSGTGTAANLVPLASQL 519 >At1g23980.1 68414.m03028 zinc finger (C3HC4-type RING finger) family protein low similarity to RING-H2 zinc finger protein ATL4 [Arabidopsis thaliana] GI:4928399; contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 369 Score = 27.1 bits (57), Expect = 8.7 Identities = 14/44 (31%), Positives = 24/44 (54%) Frame = +2 Query: 95 NHRLTRTTSKYSANVVN*ITGVIKFIFETVRPFYCFLNLLHTTV 226 NH+LT ++S S+ N I+ +I FI + + ++LH V Sbjct: 31 NHQLTDSSSSSSSGGNNRISPIILFIIVLLSVIFFICSILHLLV 74 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,866,421 Number of Sequences: 28952 Number of extensions: 251803 Number of successful extensions: 618 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 591 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 618 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1092379416 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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