Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0975.Seq
(565 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 150 5e-37
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 150 7e-37
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 150 7e-37
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.53
At1g29760.1 68414.m03639 expressed protein 31 0.53
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.1
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 6.5
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 6.5
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 6.5
At4g38440.1 68417.m05432 expressed protein 27 8.7
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 150 bits (364), Expect = 5e-37
Identities = 68/104 (65%), Positives = 82/104 (78%)
Frame = +2
Query: 254 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 433
I KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPD
Sbjct: 92 IPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPD 151
Query: 434 PLIKVNDSIQLDIATTKIMDFIKF*SGNLCMITGGRNLGRVGTI 565
PLIK ND+I+LD+ KI++FIKF GN+ M+TGGRN GRVG I
Sbjct: 152 PLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195
Score = 147 bits (356), Expect = 5e-36
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +3
Query: 3 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 182
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 183 RLIKVDGKVRTDPTYPAGFMDVVSL 257
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 150 bits (363), Expect = 7e-37
Identities = 68/104 (65%), Positives = 82/104 (78%)
Frame = +2
Query: 254 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 433
I KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPD
Sbjct: 92 IPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPD 151
Query: 434 PLIKVNDSIQLDIATTKIMDFIKF*SGNLCMITGGRNLGRVGTI 565
PLIK ND+I+LD+ KI++FIKF GN+ M+TGGRN GRVG I
Sbjct: 152 PLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195
Score = 147 bits (356), Expect = 5e-36
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +3
Query: 3 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 182
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 183 RLIKVDGKVRTDPTYPAGFMDVVSL 257
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 150 bits (363), Expect = 7e-37
Identities = 68/104 (65%), Positives = 82/104 (78%)
Frame = +2
Query: 254 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 433
I KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPD
Sbjct: 92 IPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPD 151
Query: 434 PLIKVNDSIQLDIATTKIMDFIKF*SGNLCMITGGRNLGRVGTI 565
PLIK ND+I+LD+ KI++FIKF GN+ M+TGGRN GRVG I
Sbjct: 152 PLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195
Score = 147 bits (356), Expect = 5e-36
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +3
Query: 3 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 182
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 183 RLIKVDGKVRTDPTYPAGFMDVVSL 257
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.53
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 106 KHSRNLWGPVDGLGAYTPPSLSNIHAL 26
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.53
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 217 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 125
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.1
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 196 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 80
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 6.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 129 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 227
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 6.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 129 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 227
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 6.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 129 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 227
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 27.1 bits (57), Expect = 8.7
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 9 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECL 107
+R+ P W L + V++ + STGP + E L
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELL 1172
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,770,253
Number of Sequences: 28952
Number of extensions: 297214
Number of successful extensions: 828
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 828
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1082538160
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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