Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0965.Seq
         (449 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast...    76   1e-14
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri...    74   5e-14
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta...    71   3e-13
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri...    71   5e-13
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident...    67   6e-12
At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ...    29   1.9  
At4g31850.1 68417.m04525 pentatricopeptide (PPR) repeat-containi...    27   4.4  

>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
           (MDH) identical to chloroplast NAD-malate dehydrogenase
           [Arabidopsis thaliana] GI:3256066; contains InterPro
           entry IPR001236: Lactate/malate dehydrogenase; contains
           Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
           binding domain  and PF02866: lactate/malate
           dehydrogenase, alpha/beta C-terminal domain
          Length = 403

 Score = 75.8 bits (178), Expect = 1e-14
 Identities = 35/64 (54%), Positives = 45/64 (70%)
 Frame = +3

Query: 255 LSHMNTPAKVSGTRDLRSYQAAIKDADVXVIPAGVPRKPGMTRDDLFNTNASIVRDIALS 434
           LSH NTP++V            +KD +V VIPAGVPRKPGMTRDDLFN NA+IV+ +  +
Sbjct: 126 LSHCNTPSQVRDFTGPSELADCLKDVNVVVIPAGVPRKPGMTRDDLFNINANIVKTLVEA 185

Query: 435 IAQN 446
           +A+N
Sbjct: 186 VAEN 189



 Score = 44.0 bits (99), Expect = 5e-05
 Identities = 23/33 (69%), Positives = 27/33 (81%)
 Frame = +1

Query: 157 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAAD 255
           IGQPL+LL+K +PLV+ L LYDIA V  GVAAD
Sbjct: 94  IGQPLSLLIKMSPLVSTLHLYDIANV-KGVAAD 125


>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
           putative similar to mitochondrial NAD-dependent malate
           dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 73.7 bits (173), Expect = 5e-14
 Identities = 33/63 (52%), Positives = 48/63 (76%)
 Frame = +3

Query: 255 LSHMNTPAKVSGTRDLRSYQAAIKDADVXVIPAGVPRKPGMTRDDLFNTNASIVRDIALS 434
           + H+NT ++VSG         A++ AD+ +IPAGVPRKPGMTRDDLFN NA IV++++++
Sbjct: 73  VGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIA 132

Query: 435 IAQ 443
           IA+
Sbjct: 133 IAK 135



 Score = 53.2 bits (122), Expect = 8e-08
 Identities = 33/73 (45%), Positives = 44/73 (60%), Gaps = 2/73 (2%)
 Frame = +1

Query: 43  MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 216
           MF   +  +A  V+ G   + F++ S  + K         IGQPL+LL+K NPLV+ L+L
Sbjct: 1   MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60

Query: 217 YDIAPVTPGVAAD 255
           YDIA  TPGVAAD
Sbjct: 61  YDIAN-TPGVAAD 72


>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
           putative strong similarity to glyoxysomal malate
           dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
           lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
           GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
           {Arabidopsis thaliana}, SP|P37228 {Glycine max};
           contains InterPro entry IPR001236: Lactate/malate
           dehydrogenase
          Length = 354

 Score = 71.3 bits (167), Expect = 3e-13
 Identities = 34/63 (53%), Positives = 44/63 (69%)
 Frame = +3

Query: 255 LSHMNTPAKVSGTRDLRSYQAAIKDADVXVIPAGVPRKPGMTRDDLFNTNASIVRDIALS 434
           +SHM+T A V G       + A+   D+ +IPAGVPRKPGMTRDDLFN NA IVR ++ +
Sbjct: 86  ISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEA 145

Query: 435 IAQ 443
           IA+
Sbjct: 146 IAK 148



 Score = 46.0 bits (104), Expect = 1e-05
 Identities = 24/44 (54%), Positives = 28/44 (63%)
 Frame = +1

Query: 124 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAAD 255
           FK         IGQPLA+L+K NPLV+ L LYD+A   PGV AD
Sbjct: 43  FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVAN-APGVTAD 85


>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
           identical to mitochondrial NAD-dependent malate
           dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 70.5 bits (165), Expect = 5e-13
 Identities = 32/63 (50%), Positives = 46/63 (73%)
 Frame = +3

Query: 255 LSHMNTPAKVSGTRDLRSYQAAIKDADVXVIPAGVPRKPGMTRDDLFNTNASIVRDIALS 434
           + H+NT ++V G     +   A++ AD+ +IPAGVPRKPGMTRDDLFN NA IV+++  +
Sbjct: 73  VGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTA 132

Query: 435 IAQ 443
           IA+
Sbjct: 133 IAK 135



 Score = 53.2 bits (122), Expect = 8e-08
 Identities = 30/54 (55%), Positives = 36/54 (66%)
 Frame = +1

Query: 94  KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAAD 255
           ++FS+ S    K         IGQPLALL+K NPLV+ L+LYDIA  TPGVAAD
Sbjct: 20  RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAAD 72


>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
           identical to SP|Q9ZP05; identical to cDNA microbody
           NAD-dependent malate dehydrogenase GI:3929650
          Length = 354

 Score = 66.9 bits (156), Expect = 6e-12
 Identities = 30/63 (47%), Positives = 42/63 (66%)
 Frame = +3

Query: 255 LSHMNTPAKVSGTRDLRSYQAAIKDADVXVIPAGVPRKPGMTRDDLFNTNASIVRDIALS 434
           +SHM+T A V G    +  + A+   D+ +IPAG+PRKPGMTRDDLF  NA IV+ +   
Sbjct: 86  VSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEG 145

Query: 435 IAQ 443
           +A+
Sbjct: 146 VAK 148



 Score = 41.1 bits (92), Expect = 3e-04
 Identities = 22/44 (50%), Positives = 26/44 (59%)
 Frame = +1

Query: 124 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAAD 255
           FK         IGQ L+LL+K NPLV+ L LYD+    PGV AD
Sbjct: 43  FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVN-APGVTAD 85


>At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing
          protein prolamin box binding factor, Zea mays,
          PID:g2393775
          Length = 194

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +3

Query: 3  SNIHVYTR*TNNENVLPRPKTCRPC 77
          +N++V+T   N  NV+P P+ C  C
Sbjct: 2  NNLNVFTNEDNEMNVMPPPRVCPRC 26


>At4g31850.1 68417.m04525 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 1112

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 12/34 (35%), Positives = 17/34 (50%)
 Frame = -1

Query: 287 ADLGWGVHVGKSAATPGVTGAMSYKASLVTRGFC 186
           A+L W   +G   A  G+  A+S+   LV  G C
Sbjct: 712 ANLFWEDLIGSILAEAGIDNAVSFSERLVANGIC 745


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,330,401
Number of Sequences: 28952
Number of extensions: 234498
Number of successful extensions: 572
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 572
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)