BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0903.Seq (499 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g29190.1 68415.m03548 pumilio/Puf RNA-binding domain-containi... 38 0.005 At2g29200.1 68415.m03549 pumilio/Puf RNA-binding domain-containi... 37 0.007 At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containi... 37 0.007 At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containi... 36 0.020 At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containi... 32 0.19 At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containi... 32 0.19 At5g43110.1 68418.m05263 pumilio/Puf RNA-binding domain-containi... 29 1.3 At3g12850.1 68416.m01602 COP9 signalosome complex-related / CSN ... 29 2.3 At4g08560.1 68417.m01408 pumilio/Puf RNA-binding domain-containi... 28 3.0 At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transfera... 28 4.0 At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi... 27 7.0 At5g17180.1 68418.m02013 hypothetical protein 27 9.3 At1g50730.1 68414.m05705 expressed protein 27 9.3 >At2g29190.1 68415.m03548 pumilio/Puf RNA-binding domain-containing protein Length = 972 Score = 37.5 bits (83), Expect = 0.005 Identities = 20/45 (44%), Positives = 27/45 (60%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILKYG 240 VIQ +L+H D I KEL IVQM Q K+A + V++ L +G Sbjct: 832 VIQHVLEHGKPDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFG 876 >At2g29200.1 68415.m03549 pumilio/Puf RNA-binding domain-containing protein similar to BPM [Hordeum vulgare] GI:20513851 Length = 968 Score = 37.1 bits (82), Expect = 0.007 Identities = 19/45 (42%), Positives = 27/45 (60%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILKYG 240 V+Q +L+H D I KEL IVQM Q K+A + V++ L +G Sbjct: 828 VVQHVLEHGKPDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFG 872 Score = 28.7 bits (61), Expect = 2.3 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = +3 Query: 261 VLKKFYGHIVSLSTHAIKC 317 ++ F+GH+V+LSTH C Sbjct: 771 IISTFFGHVVTLSTHPYGC 789 >At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containing protein Length = 964 Score = 37.1 bits (82), Expect = 0.007 Identities = 19/45 (42%), Positives = 27/45 (60%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILKYG 240 V+Q +L+H D I KEL IVQM Q K+A + V++ L +G Sbjct: 824 VVQHVLEHGKPDERTVIIKELAGKIVQMSQQKFASNVVEKCLTFG 868 Score = 28.7 bits (61), Expect = 2.3 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = +3 Query: 261 VLKKFYGHIVSLSTHAIKC 317 ++ F+GH+V+LSTH C Sbjct: 767 IISTFFGHVVTLSTHPYGC 785 >At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containing protein contains Pfam profile:PF00806 Pumilio-family RNA binding domains Length = 641 Score = 35.5 bits (78), Expect = 0.020 Identities = 21/77 (27%), Positives = 39/77 (50%) Frame = +1 Query: 1 RKVVKSDFKINSCKELHNMLKGQYKSIALTHDLSRVIQVLLKHSPEDIINEITKELLDII 180 R + K D + E +KG+ IA++H SRV+Q +K + + + EL Sbjct: 101 RNIGKED-RSKLISEAIRKMKGKVPEIAVSHVSSRVLQTCVKFCSQAEKDVLFTELQPQF 159 Query: 181 VQMGQSKYAHHSVKRIL 231 + + +KYA H ++++L Sbjct: 160 LNLASNKYAVHFIQKML 176 >At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containing protein contains Pfam profile: PF00806 Pumilio-family RNA binding domains (aka PUM-HD, Pumilio homology domain) (8 copies at C-terminus) Length = 961 Score = 32.3 bits (70), Expect = 0.19 Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 2/56 (3%) Frame = +1 Query: 76 SIALTHDL--SRVIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILKY 237 + AL HD + V Q +L+ D +I ++L +VQM Q KYA + V++ L++ Sbjct: 814 AFALAHDQYGNYVTQHVLERGKPDERRQIIEKLTGNVVQMSQHKYASNVVEKCLEH 869 >At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containing protein similar to RNA binding protein PufA GB:AAD39751 [Dictyostelium discoideum] and similar to Pumilio protein GB:A46221 [Drosophila sp.] Length = 1003 Score = 32.3 bits (70), Expect = 0.19 Identities = 16/45 (35%), Positives = 27/45 (60%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILKYG 240 VIQ +++H +EI +L IV+M Q K+A + V++ L +G Sbjct: 862 VIQHIIQHGKPHERSEIINKLAGQIVKMSQQKFASNVVEKCLTFG 906 >At5g43110.1 68418.m05263 pumilio/Puf RNA-binding domain-containing protein contains similarity to RNA-binding protein Length = 518 Score = 29.5 bits (63), Expect = 1.3 Identities = 10/43 (23%), Positives = 28/43 (65%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILK 234 V+Q +++ + +I+ + ++L+ + ++KY H+V+++LK Sbjct: 405 VVQYIVELNNRYLIDALVRQLIGNYAHLARNKYGSHAVQKLLK 447 >At3g12850.1 68416.m01602 COP9 signalosome complex-related / CSN complex-related low similarity to SP|P45432 COP9 signalosome complex subunit 1 (CSN complex subunit 1) (COP11 protein) (FUSCA protein FUS6) {Arabidopsis thaliana} Length = 367 Score = 28.7 bits (61), Expect = 2.3 Identities = 20/80 (25%), Positives = 41/80 (51%), Gaps = 1/80 (1%) Frame = +1 Query: 22 FKINSCKELHNMLKGQYKSIALTHDLSRVIQVLLKHSPEDIINEITKELLDIIVQMGQSK 201 FKI+ K L N+L+ +Y+ A + + ++ +P I ++ L ++I G Sbjct: 187 FKIDCFKSLSNLLESKYQVTAEGNAVDTIV------NP---IGQVASHLENVIKPFGPCD 237 Query: 202 YAHHSVKRILK-YGTDCISM 258 Y +K++L + TDC+++ Sbjct: 238 YLIDVIKKLLAGHFTDCMTL 257 >At4g08560.1 68417.m01408 pumilio/Puf RNA-binding domain-containing protein low similarity to RNA binding protein PufA [Dictyostelium discoideum] GI:5106561; contains Pfam profile PF00806: Pumilio-family RNA binding repeat Length = 477 Score = 28.3 bits (60), Expect = 3.0 Identities = 13/43 (30%), Positives = 24/43 (55%) Frame = +1 Query: 106 VIQVLLKHSPEDIINEITKELLDIIVQMGQSKYAHHSVKRILK 234 V+Q LL+ ++ + ++K L VQ+ KY H V++ L+ Sbjct: 361 VVQYLLRLKDYEVTSALSKHLDGNYVQLSYDKYGSHVVQKCLE 403 >At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 489 Score = 27.9 bits (59), Expect = 4.0 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = -2 Query: 294 GIQYVHKTSSKPHANAICSIFQYSLNGMMRILRLTH 187 G Q +H S KPH + Q +N MMR+ +L H Sbjct: 2 GSQIIHN-SQKPHVVCVPYPAQGHINPMMRVAKLLH 36 >At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing protein contains Pfam profile: PF00806 Pumilio-family RNA binding domains (aka PUM-HD, Pumilio homology domain) (8 copies at C-terminal half of protein) Length = 650 Score = 27.1 bits (57), Expect = 7.0 Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 2/60 (3%) Frame = +1 Query: 58 LKGQYKSIALTHDLSRVIQVLLKHSPEDIINEITKELLDI--IVQMGQSKYAHHSVKRIL 231 LKG Y +++ S +++ L H PE +I +EL+ + Q+ Q YA+ ++ L Sbjct: 554 LKGHYVQLSMQKFSSHMVERCLMHCPES-RPQIVRELVSVPHFDQLLQDPYANFVIQAAL 612 >At5g17180.1 68418.m02013 hypothetical protein Length = 123 Score = 26.6 bits (56), Expect = 9.3 Identities = 10/17 (58%), Positives = 13/17 (76%) Frame = +1 Query: 115 VLLKHSPEDIINEITKE 165 V LKH P+D+IN + KE Sbjct: 77 VSLKHKPKDVINVVQKE 93 >At1g50730.1 68414.m05705 expressed protein Length = 1013 Score = 26.6 bits (56), Expect = 9.3 Identities = 10/40 (25%), Positives = 25/40 (62%) Frame = +1 Query: 61 KGQYKSIALTHDLSRVIQVLLKHSPEDIINEITKELLDII 180 + +++S + +S ++ LLK P ++++ + E+LD+I Sbjct: 404 RNKFQSSYNSSHVSILLHYLLKELPSELVSSLAMEILDMI 443 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,820,700 Number of Sequences: 28952 Number of extensions: 179964 Number of successful extensions: 489 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 471 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 489 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -