Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0885.Seq
(329 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g23630.1 68417.m03403 reticulon family protein (RTNLB1) weak ... 44 2e-05
At4g11220.1 68417.m01818 reticulon family protein (RTNLB2) simil... 42 1e-04
At5g41600.1 68418.m05054 reticulon family protein (RTNLB4) weak ... 41 2e-04
At3g18260.1 68416.m02323 reticulon family protein (RTNLB9) weak ... 38 0.002
At1g64090.1 68414.m07260 reticulon family protein (RTNLB3) weak ... 35 0.015
At2g46170.1 68415.m05741 reticulon family protein (RTNLB5) weak ... 34 0.020
At3g10260.3 68416.m01230 reticulon family protein weak similarit... 34 0.026
At3g10260.2 68416.m01229 reticulon family protein weak similarit... 34 0.026
At3g10260.1 68416.m01228 reticulon family protein weak similarit... 34 0.026
At3g61560.1 68416.m06895 reticulon family protein (RTNLB6) conta... 33 0.046
At3g19460.1 68416.m02467 reticulon family protein (RTNLB11) weak... 33 0.061
At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein le... 27 4.0
At3g60600.1 68416.m06781 vesicle-associated membrane protein, pu... 26 5.3
At5g11430.1 68418.m01335 transcription elongation factor-related... 26 7.0
>At4g23630.1 68417.m03403 reticulon family protein (RTNLB1) weak
similarity to Nogo-C protein [Rattus norvegicus]
GI:6822251; contains Pfam profile PF02453: Reticulon
Length = 275
Score = 44.0 bits (99), Expect = 2e-05
Identities = 25/73 (34%), Positives = 40/73 (54%), Gaps = 4/73 (5%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINE----ITAKVRA 90
VL+ +G CFN +TL + + LFT+P AY+ + +VD E A ++ + + KV +
Sbjct: 203 VLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLDEKVLS 262
Query: 89 AVPLGRRAEGDKD 51
+PLG KD
Sbjct: 263 KIPLGPLKNKKKD 275
>At4g11220.1 68417.m01818 reticulon family protein (RTNLB2) similar
to SP|Q64548 Reticulon 1 (Neuroendocrine-specific
protein) {Rattus norvegicus}; contains Pfam profile
PF02453: Reticulon
Length = 271
Score = 41.5 bits (93), Expect = 1e-04
Identities = 23/73 (31%), Positives = 42/73 (57%), Gaps = 4/73 (5%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINE----ITAKVRA 90
VL+ +G C++ +TL + + LFT+P Y+ + +VD+ E A A++ + + AKV +
Sbjct: 199 VLSILGGCYSFLTLAYIALVLLFTVPLFYDKYEDKVDSYGEKAMAELKKQYAVLDAKVFS 258
Query: 89 AVPLGRRAEGDKD 51
+P G + KD
Sbjct: 259 KIPRGPLKDKKKD 271
>At5g41600.1 68418.m05054 reticulon family protein (RTNLB4) weak
similarity to Nogo-C protein [Rattus norvegicus]
GI:6822251, SP|O95197 Reticulon protein 3
(Neuroendocrine-specific protein-like) {Homo sapiens};
contains Pfam profile PF02453: Reticulon
Length = 257
Score = 40.7 bits (91), Expect = 2e-04
Identities = 19/49 (38%), Positives = 30/49 (61%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINE 111
VL+ +G+C+N +TL + LFT+P YE + +VDA E A +I +
Sbjct: 182 VLSIIGSCYNFLTLFYTATVLLFTIPVLYEKYEDKVDAYGEKAMREIKK 230
>At3g18260.1 68416.m02323 reticulon family protein (RTNLB9) weak
similarity to RTN2-C [Homo sapiens] GI:3435090; contains
Pfam profile PF02453: Reticulon
Length = 225
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/54 (29%), Positives = 31/54 (57%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINEITAKV 96
+++ +G FN + L+ +G++++ TLP YEM + VD K+ ++ KV
Sbjct: 153 IVSIIGTYFNFVNLLFIGFVSMQTLPVMYEMYEDDVDTAAGKLMRKMKKLYKKV 206
>At1g64090.1 68414.m07260 reticulon family protein (RTNLB3) weak
similarity to SP|O95197 Reticulon protein 3
(Neuroendocrine-specific protein-like) {Homo sapiens};
contains Pfam profile PF02453: Reticulon
Length = 255
Score = 34.7 bits (76), Expect = 0.015
Identities = 19/49 (38%), Positives = 29/49 (59%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINE 111
VL+ VG+ N +TLI + + LFT+P YE + +VD E A +I +
Sbjct: 178 VLSKVGSSCNFLTLIYIATVLLFTIPVLYEKYEDKVDDFGEKAMREIKK 226
>At2g46170.1 68415.m05741 reticulon family protein (RTNLB5) weak
similarity to Nogo-C protein [Rattus norvegicus]
GI:6822251; contains Pfam profile PF02453: Reticulon
Length = 255
Score = 34.3 bits (75), Expect = 0.020
Identities = 15/49 (30%), Positives = 30/49 (61%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINE 111
+++ VG FN +TL+ + ++ L T+P YE ++ +VD E A ++ +
Sbjct: 182 IISVVGNWFNFLTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQK 230
>At3g10260.3 68416.m01230 reticulon family protein weak similarity
to Nogo-C protein [Rattus norvegicus] GI:6822251;
contains Pfam profile PF02453: Reticulon; identical to
cDNA GI:32331854
Length = 267
Score = 33.9 bits (74), Expect = 0.026
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLE 135
V VG+C N +T++ +G++ T+P YE + +VD ++
Sbjct: 195 VAAMVGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMD 235
>At3g10260.2 68416.m01229 reticulon family protein weak similarity
to Nogo-C protein [Rattus norvegicus] GI:6822251;
contains Pfam profile PF02453: Reticulon; identical to
cDNA GI:32331854
Length = 247
Score = 33.9 bits (74), Expect = 0.026
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLE 135
V VG+C N +T++ +G++ T+P YE + +VD ++
Sbjct: 175 VAAMVGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMD 215
>At3g10260.1 68416.m01228 reticulon family protein weak similarity
to Nogo-C protein [Rattus norvegicus] GI:6822251;
contains Pfam profile PF02453: Reticulon; identical to
cDNA GI:32331854
Length = 247
Score = 33.9 bits (74), Expect = 0.026
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLE 135
V VG+C N +T++ +G++ T+P YE + +VD ++
Sbjct: 175 VAAMVGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMD 215
>At3g61560.1 68416.m06895 reticulon family protein (RTNLB6) contains
Pfam profile PF02453: Reticulon
Length = 253
Score = 33.1 bits (72), Expect = 0.046
Identities = 13/37 (35%), Positives = 25/37 (67%)
Frame = -1
Query: 257 VLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVD 147
+++ VG FN +TL+ + ++ L T+P YE ++ +VD
Sbjct: 182 IISVVGNWFNFLTLVYICFVVLHTVPMLYEKHEDKVD 218
>At3g19460.1 68416.m02467 reticulon family protein (RTNLB11) weak
similarity to neuroendocrine-specific protein C [Homo
sapiens] GI:307311; identical to cDNA RTNLB11
GI:32331878
Length = 200
Score = 32.7 bits (71), Expect = 0.061
Identities = 15/62 (24%), Positives = 28/62 (45%)
Frame = -1
Query: 284 LAEVRRAAVVLTYVGACFNGITLIILGWIALFTLPKAYEMNKAQVDANLELARAKINEIT 105
L +V ++YVG N +TL+ +G + + P YE + +D + + I+
Sbjct: 127 LLQVSLVLWAISYVGTLINSLTLVYIGVLLSLSFPIVYEKYQDHIDEKVNSTSKFVRNIS 186
Query: 104 AK 99
K
Sbjct: 187 RK 188
>At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein
leucine zipper-containing protein - Lycopersicon
esculentum, EMBL:Z12127 contains Pfam domain PF03081:
Exo70 exocyst complex subunit;
Length = 653
Score = 26.6 bits (56), Expect = 4.0
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 210 DDERDPVEARPHVREHHSSTPNFSESTXSSTRNSLAQVIQ 329
D + + E + H E HSSTP ES S+ +SL +V++
Sbjct: 18 DHKSEDHENKQHSDELHSSTP---ESQSESSEHSLVEVME 54
>At3g60600.1 68416.m06781 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 256
Score = 26.2 bits (55), Expect = 5.3
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -1
Query: 164 NKAQVDANLELARAKINEITAKVRAAVPLGRRAEGDKDK 48
NKA N ARA I ++T + ++A+ L R + + D+
Sbjct: 183 NKAGHQENTSEARALITKLTEEKQSAIQLNNRLQRELDQ 221
>At5g11430.1 68418.m01335 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 871
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 198 YPTEDDERDPVEARPHVREHHSSTP 272
Y +DDE D E +PHV H P
Sbjct: 806 YDDDDDEDDIPEWQPHVPSHQLPPP 830
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,261,371
Number of Sequences: 28952
Number of extensions: 52084
Number of successful extensions: 227
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 380568784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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