BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0802.Seq (422 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 77 5e-15 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 74 5e-14 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 74 5e-14 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 74 5e-14 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 51 3e-07 At3g63180.1 68416.m07097 expressed protein 28 2.3 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 27 4.0 At2g04440.1 68415.m00448 NUDIX/mutT hydrolase family protein sim... 27 4.0 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 27 4.0 At5g41320.1 68418.m05022 expressed protein 27 6.9 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 77.0 bits (181), Expect = 5e-15 Identities = 30/46 (65%), Positives = 39/46 (84%) Frame = -2 Query: 391 TPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 254 TP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDHW+++ DP Sbjct: 764 TPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDP 809 Score = 27.9 bits (59), Expect = 3.0 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = -1 Query: 257 PCEPQSKPYNVVQETRKRKGLKEGL 183 P EP ++ +V + RKRKGLKE + Sbjct: 809 PLEPGTQASVLVADIRKRKGLKEAM 833 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 73.7 bits (173), Expect = 5e-14 Identities = 33/51 (64%), Positives = 38/51 (74%) Frame = -2 Query: 406 VPGGRTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 254 VP TP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP Sbjct: 861 VPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDP 911 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 73.7 bits (173), Expect = 5e-14 Identities = 33/51 (64%), Positives = 38/51 (74%) Frame = -2 Query: 406 VPGGRTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 254 VP TP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP Sbjct: 875 VPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDP 925 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 73.7 bits (173), Expect = 5e-14 Identities = 33/51 (64%), Positives = 38/51 (74%) Frame = -2 Query: 406 VPGGRTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 254 VP TP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP Sbjct: 875 VPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDP 925 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 51.2 bits (117), Expect = 3e-07 Identities = 22/44 (50%), Positives = 28/44 (63%) Frame = -2 Query: 385 MFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 254 +F V AY+PV+ESFGF +LR T G A V HW++L DP Sbjct: 915 LFTVHAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958 >At3g63180.1 68416.m07097 expressed protein Length = 978 Score = 28.3 bits (60), Expect = 2.3 Identities = 12/45 (26%), Positives = 23/45 (51%) Frame = -3 Query: 369 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSSLETLRTSEQA 235 P ++ + L P+ VP+ + RP++S + +G +S S A Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLASANFSHPSSSA 583 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 27.5 bits (58), Expect = 4.0 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = -2 Query: 379 IVKAYLPVNESFGFTADLRSNTGGQ 305 ++ A +P+N FG++ LRS T G+ Sbjct: 698 VITANVPLNNMFGYSTSLRSMTQGK 722 >At2g04440.1 68415.m00448 NUDIX/mutT hydrolase family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 215 Score = 27.5 bits (58), Expect = 4.0 Identities = 19/48 (39%), Positives = 24/48 (50%), Gaps = 2/48 (4%) Frame = -2 Query: 352 ESFGFTADLRSN-TGGQAFPQCVFDHWQVLPGDPANLRASP-TTLYRK 215 E GF+ L N TG +++ C DH L G P + S TTL RK Sbjct: 164 EYLGFSNVLTKNSTGKESYLYCSTDHAYFLKGKPDHSSTSLFTTLLRK 211 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 27.5 bits (58), Expect = 4.0 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = -2 Query: 379 IVKAYLPVNESFGFTADLRSNTGGQ 305 ++ A +P+N FG++ LRS T G+ Sbjct: 698 VITANVPLNNMFGYSTSLRSMTQGK 722 >At5g41320.1 68418.m05022 expressed protein Length = 515 Score = 26.6 bits (56), Expect = 6.9 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Frame = -3 Query: 327 CVPTPADRPSRSAYSTIGRSSLETLRTSEQAL--QRCTGNEKEERIEGR 187 C P DR S S +GR+ + + S++AL Q N K E ++G+ Sbjct: 253 CNGGPLDRFLHSGQSGLGRNYFQQQQESQEALVAQTEMDNSKNETVKGQ 301 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,048,701 Number of Sequences: 28952 Number of extensions: 183533 Number of successful extensions: 520 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 507 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 520 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 655255392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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