Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0801.Seq
(438 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204; Euka... 97 2e-19
UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56; Euk... 94 1e-18
UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2; Ost... 86 3e-16
UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4; Bilateria|... 86 4e-16
UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3; Eukary... 85 8e-16
UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3; Eukary... 64 1e-09
UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1; ... 52 4e-06
UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1; Enceph... 52 4e-06
UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiel... 51 1e-05
UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole ge... 44 0.001
UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S riboso... 39 0.054
UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102... 37 0.22
UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse t... 33 2.7
UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1; ... 33 2.7
UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella ve... 32 6.2
>UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204;
Eukaryota|Rep: 60S ribosomal protein L27a - Homo sapiens
(Human)
Length = 148
Score = 96.7 bits (230), Expect = 2e-19
Identities = 40/75 (53%), Positives = 51/75 (68%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
M + +KTRKLRGHVS NAGG HHHRIN DKYHPGYFGK+GM++
Sbjct: 1 MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKH 60
Query: 211 FHFRKNKNFCPVLNL 255
+H ++N++FCP +NL
Sbjct: 61 YHLKRNQSFCPTVNL 75
Score = 57.6 bits (133), Expect = 1e-07
Identities = 28/64 (43%), Positives = 37/64 (57%)
Frame = +3
Query: 210 FPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 389
+ L + Q F + LDKLWTLVSEQTR+ A G P+I++V++ PKQ
Sbjct: 61 YHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQ 120
Query: 390 PVIV 401
PVIV
Sbjct: 121 PVIV 124
>UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56;
Eukaryota|Rep: 60S ribosomal protein L27a-2 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 146
Score = 94.3 bits (224), Expect = 1e-18
Identities = 43/75 (57%), Positives = 48/75 (64%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
MAT+ KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 211 FHFRKNKNFCPVLNL 255
FH +NK FCP++NL
Sbjct: 61 FHKLRNKFFCPIVNL 75
>UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2;
Ostreococcus|Rep: 60s ribosomal protein L15/L27 -
Ostreococcus tauri
Length = 198
Score = 86.2 bits (204), Expect = 3e-16
Identities = 38/73 (52%), Positives = 44/73 (60%)
Frame = +1
Query: 37 TSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFH 216
T K RK RGHVS AGG+HHHRI MDKYHPGYFGK+GMR+FH
Sbjct: 54 TQLHKNRKKRGHVSAGHGRIGKHRKHPAGRGMAGGQHHHRIMMDKYHPGYFGKVGMRHFH 113
Query: 217 FRKNKNFCPVLNL 255
KN+ CP +N+
Sbjct: 114 KLKNQTVCPTINI 126
Score = 33.9 bits (74), Expect = 1.5
Identities = 19/60 (31%), Positives = 27/60 (45%)
Frame = +3
Query: 222 KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIV 401
K Q + +DKLWTLV + R + A + PVI++ P QPV+V
Sbjct: 116 KNQTVCPTINIDKLWTLVGTEKREECAK-NTSQAPVIDLTDHGIFKLLGKGQLPNQPVVV 174
>UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4;
Bilateria|Rep: Ribosomal protein L27a - Ascaris suum
(Pig roundworm) (Ascaris lumbricoides)
Length = 133
Score = 85.8 bits (203), Expect = 4e-16
Identities = 33/42 (78%), Positives = 37/42 (88%)
Frame = +1
Query: 130 NAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNL 255
NAGG+HHHRIN DKYHPGYFGK+GMRNFH RKN +CP +NL
Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRNFHVRKNGLYCPTVNL 61
Score = 54.4 bits (125), Expect = 1e-06
Identities = 30/64 (46%), Positives = 37/64 (57%)
Frame = +3
Query: 210 FPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 389
F + K + + LDKLW+LVSE+TR YA D K PVI++VKA PKQ
Sbjct: 47 FHVRKNGLYCPTVNLDKLWSLVSEKTRTTYAEKKD-KAPVIDVVKAGYFKVLGKGLLPKQ 105
Query: 390 PVIV 401
PVIV
Sbjct: 106 PVIV 109
>UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3;
Eukaryota|Rep: 60S ribosomal protein L27a - Tetrahymena
thermophila
Length = 149
Score = 84.6 bits (200), Expect = 8e-16
Identities = 40/77 (51%), Positives = 47/77 (61%), Gaps = 2/77 (2%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXN--AGGEHHHRINMDKYHPGYFGKLGM 204
M + KKTRKLRGHVS AGG HHHRI M+K+HPGY+GKLGM
Sbjct: 1 MVSHLKKTRKLRGHVSHGHGRVGKHRKGGCRGGRGKAGGMHHHRILMEKWHPGYYGKLGM 60
Query: 205 RNFHFRKNKNFCPVLNL 255
R FH +KN CPV+N+
Sbjct: 61 RTFHLKKNPLHCPVVNI 77
Score = 47.2 bits (107), Expect = 2e-04
Identities = 24/50 (48%), Positives = 30/50 (60%)
Frame = +3
Query: 252 LDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIV 401
+DKLW+LVS+ TR KYA KVPVI++ KA P QPV+V
Sbjct: 77 IDKLWSLVSDATRQKYAE-DKKKVPVIDVTKAGFFKVLGKGRLPNQPVVV 125
>UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3;
Eukaryota|Rep: 60S ribosomal protein L27A - Guillardia
theta (Cryptomonas phi)
Length = 144
Score = 64.1 bits (149), Expect = 1e-09
Identities = 30/75 (40%), Positives = 40/75 (53%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
M T +++RK RGHVS N+GG+HHH+I +KYH +FGK GMR
Sbjct: 1 MPTDIRRSRKKRGHVSSGHGRVGKHRKHPGGRGNSGGQHHHKIFFEKYHKKHFGKTGMRY 60
Query: 211 FHFRKNKNFCPVLNL 255
+ K NF +NL
Sbjct: 61 YRKLKQNNFNKSINL 75
>UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 187
Score = 52.4 bits (120), Expect = 4e-06
Identities = 25/50 (50%), Positives = 26/50 (52%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHP 180
M T KTRK RGHVS AGG+HHHR NMDKYHP
Sbjct: 1 MPTHLSKTRKHRGHVSAGNGRVGKHRKHPGGRGLAGGQHHHRTNMDKYHP 50
Score = 40.7 bits (91), Expect = 0.013
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +1
Query: 139 GEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNL 255
G ++ + YFGK+GMR+FH +N +F PV+NL
Sbjct: 77 GSQEMEVDREIGRDSYFGKVGMRHFHLTRNSSFRPVVNL 115
Score = 34.7 bits (76), Expect = 0.87
Identities = 23/64 (35%), Positives = 29/64 (45%)
Frame = +3
Query: 210 FPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 389
F L + F LDKLWTLV + R K +A +VPVI+ + A P
Sbjct: 101 FHLTRNSSFRPVVNLDKLWTLVPAEQR-KGLTADSTEVPVIDTLAAGYGKVLGKGRLPAL 159
Query: 390 PVIV 401
P IV
Sbjct: 160 PCIV 163
>UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1;
Encephalitozoon cuniculi|Rep: 60S ribosomal protein L27a
- Encephalitozoon cuniculi
Length = 147
Score = 52.4 bits (120), Expect = 4e-06
Identities = 26/69 (37%), Positives = 34/69 (49%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
M KKTRKLRGHVS AGG H + ++HP Y GK GMR
Sbjct: 1 MTDRVKKTRKLRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRV 60
Query: 211 FHFRKNKNF 237
+H ++N ++
Sbjct: 61 YHRKENSDY 69
>UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiella
natans|Rep: Ribosomal protein L27a - Bigelowiella natans
(Pedinomonas minutissima) (Chlorarachnion sp.(strain
CCMP 621))
Length = 151
Score = 50.8 bits (116), Expect = 1e-05
Identities = 24/68 (35%), Positives = 33/68 (48%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
M T KK+RK+RG S +GG+HHH+I ++Y YF K GMR
Sbjct: 1 MVTKLKKSRKMRGKNSSGHGRINKHRKNPGGKGKSGGQHHHKIIFERYRETYFRKTGMRI 60
Query: 211 FHFRKNKN 234
++ K N
Sbjct: 61 YNLEKTLN 68
>UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole
genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome chr12 scaffold_159, whole genome shotgun
sequence - Vitis vinifera (Grape)
Length = 151
Score = 44.0 bits (99), Expect = 0.001
Identities = 16/30 (53%), Positives = 24/30 (80%)
Frame = +1
Query: 166 DKYHPGYFGKLGMRNFHFRKNKNFCPVLNL 255
DK+HPG FGK+GMR+FH + K CP++++
Sbjct: 28 DKHHPGNFGKVGMRHFHKLRIKLHCPIVHI 57
>UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S ribosomal
protein L27a; n=1; Rattus norvegicus|Rep: PREDICTED:
similar to 60S ribosomal protein L27a - Rattus
norvegicus
Length = 86
Score = 38.7 bits (86), Expect = 0.054
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPG 183
M+ +KTRK +GHVS NAGGEH HRIN PG
Sbjct: 1 MSGRLRKTRKFQGHVSDSHSRISKHHKHRGGQRNAGGEHLHRINCYYKVPG 51
>UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102C;
n=2; Saccharomycetaceae|Rep: Putative uncharacterized
protein YGL102C - Saccharomyces cerevisiae (Baker's
yeast)
Length = 142
Score = 36.7 bits (81), Expect = 0.22
Identities = 19/58 (32%), Positives = 29/58 (50%)
Frame = -1
Query: 255 QVQNWTEILVLSKVEISHTKFAKVSRMVLVHVDXXXXXXXXXXXXXXXXXVLSDTTMS 82
QVQ+W + L VE+SHT +++ MV +HV+ VL+DTT +
Sbjct: 78 QVQDWLPEMSLLLVEVSHTNLTEITWMVFIHVNSVVMLTTGHTSTTGVLSVLTDTTFT 135
>UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse
transcriptase; n=1; Arabidopsis thaliana|Rep: Putative
non-LTR retroelement reverse transcriptase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 1044
Score = 33.1 bits (72), Expect = 2.7
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 165 GQVPSWILWQTWYEKFPL*KEQEFLSSFEL 254
GQ+P WILW W + L EQ+ +SS +L
Sbjct: 842 GQLPIWILWNLWNCRNKLIFEQKHISSMDL 871
>UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1;
n=1; Arabidopsis thaliana|Rep: Putative 60S ribosomal
protein L27a-1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 104
Score = 33.1 bits (72), Expect = 2.7
Identities = 27/77 (35%), Positives = 34/77 (44%), Gaps = 2/77 (2%)
Frame = +1
Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 204
M TS+KKTR LR HVS H R + PG G +GM
Sbjct: 1 MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39
Query: 205 RNFHFRKNKNFCPVLNL 255
R FH +NK +C ++NL
Sbjct: 40 RYFHKLRNKFYCQIVNL 56
>UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 306
Score = 31.9 bits (69), Expect = 6.2
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = -3
Query: 238 RNSCSF*SGNFSYQVCQSIQDGTCPC*FCDGAHHQHYHDLLDAYGA 101
R+SC + F +C+S T PC D H +H DL+D GA
Sbjct: 108 RSSCQ--ARCFMNNLCRSYNYNTTPCQLSDSDHLEHPSDLVDKPGA 151
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 409,602,504
Number of Sequences: 1657284
Number of extensions: 7781004
Number of successful extensions: 19416
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 18790
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19398
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 21918499148
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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