BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0786.Seq (424 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g09800.1 68417.m01609 40S ribosomal protein S18 (RPS18C) 110 3e-25 At1g34030.1 68414.m04219 40S ribosomal protein S18 (RPS18B) simi... 110 3e-25 At1g22780.1 68414.m02846 40S ribosomal protein S18 (RPS18A) Matc... 110 3e-25 At5g14320.1 68418.m01674 30S ribosomal protein S13, chloroplast ... 32 0.14 At1g61710.1 68414.m06960 DC1 domain-containing protein contains ... 29 0.98 At1g49890.1 68414.m05593 expressed protein contains Pfam domain,... 29 1.3 At5g49610.1 68418.m06139 F-box family protein contains Pfam PF00... 29 1.7 At1g77750.1 68414.m09052 30S ribosomal protein S13, chloroplast,... 29 1.7 At4g10960.1 68417.m01781 UDP-glucose 4-epimerase, putative / UDP... 28 2.3 At3g07210.1 68416.m00860 expressed protein predicted using genef... 28 3.0 At3g14870.1 68416.m01880 expressed protein contains Pfam profile... 27 5.2 At5g18680.1 68418.m02217 F-box family protein / tubby family pro... 27 6.9 At5g04920.1 68418.m00519 vacuolar protein sorting 36 family prot... 26 9.1 >At4g09800.1 68417.m01609 40S ribosomal protein S18 (RPS18C) Length = 152 Score = 110 bits (265), Expect = 3e-25 Identities = 48/58 (82%), Positives = 52/58 (89%) Frame = -1 Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80 KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135 Score = 65.3 bits (152), Expect = 2e-11 Identities = 26/47 (55%), Positives = 40/47 (85%) Frame = -2 Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250 +IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ + Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78 >At1g34030.1 68414.m04219 40S ribosomal protein S18 (RPS18B) similar to ribosomal protein S18 GI:38422 from [Homo sapiens] Length = 152 Score = 110 bits (265), Expect = 3e-25 Identities = 48/58 (82%), Positives = 52/58 (89%) Frame = -1 Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80 KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135 Score = 65.3 bits (152), Expect = 2e-11 Identities = 26/47 (55%), Positives = 40/47 (85%) Frame = -2 Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250 +IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ + Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78 >At1g22780.1 68414.m02846 40S ribosomal protein S18 (RPS18A) Match to ribosomal S18 gene mRNA gb|Z28701, DNA gb|Z23165 from A. thaliana. ESTs gb|T21121, gb|Z17755, gb|R64776 and gb|R30430 come from this gene Length = 152 Score = 110 bits (265), Expect = 3e-25 Identities = 48/58 (82%), Positives = 52/58 (89%) Frame = -1 Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80 KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135 Score = 65.3 bits (152), Expect = 2e-11 Identities = 26/47 (55%), Positives = 40/47 (85%) Frame = -2 Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250 +IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ + Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78 >At5g14320.1 68418.m01674 30S ribosomal protein S13, chloroplast (CS13) ribosomal protein S13 precursor, chloroplast Arabidopsis thaliana, PIR:S59594; identical to cDNA ribosomal protein S13 GI:1515106 Length = 169 Score = 32.3 bits (70), Expect = 0.14 Identities = 13/24 (54%), Positives = 19/24 (79%) Frame = -1 Query: 154 LERLKKIRAHRGMRHYWGLRVRGQ 83 ++RLK+I+ +RG+RH GL RGQ Sbjct: 122 IKRLKEIQCYRGVRHIQGLPCRGQ 145 >At1g61710.1 68414.m06960 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 402 Score = 29.5 bits (63), Expect = 0.98 Identities = 14/40 (35%), Positives = 24/40 (60%) Frame = +1 Query: 286 FFNLFFCAFASTLVKINVGFFENNVGVPPANTFDSRIANI 405 FFNLFF A +KI +G F ++ +PP+++ + +I Sbjct: 34 FFNLFFYAQVHENLKIELGIFSRSI-LPPSSSSLQEVLHI 72 >At1g49890.1 68414.m05593 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 659 Score = 29.1 bits (62), Expect = 1.3 Identities = 14/38 (36%), Positives = 23/38 (60%) Frame = +2 Query: 290 STSSSVHSPARLSRSMSAFLRTMLEYLRPTPLIAASQT 403 +T+++ + + S S SA LRT Y P+PL++ S T Sbjct: 56 TTTTTTTTSSSSSSSSSAILRTSKRYPSPSPLLSRSTT 93 >At5g49610.1 68418.m06139 F-box family protein contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box protein interaction domain; similar to unknown protein (gb|AAF30317.1) Length = 359 Score = 28.7 bits (61), Expect = 1.7 Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -2 Query: 111 ITGAF-VCVVSTLRLLAGEEELLVYQRRSKLNK 16 + G F +C LA +++LVYQRRSKL K Sbjct: 299 VPGIFPICQTGEYVFLATHKQVLVYQRRSKLWK 331 >At1g77750.1 68414.m09052 30S ribosomal protein S13, chloroplast, putative similar to putative 30S ribosomal protein S13, chloroplast precursor GB:P42732 [Arabidopsis thaliana] Length = 154 Score = 28.7 bits (61), Expect = 1.7 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = -1 Query: 178 LDSKLREDLERLKKIRAHRGMRHYWGLRVRGQ 83 L ++ +++RL ++ +RG RH GL RGQ Sbjct: 98 LRRRVGSEIQRLVEVDCYRGSRHRHGLPCRGQ 129 >At4g10960.1 68417.m01781 UDP-glucose 4-epimerase, putative / UDP-galactose 4-epimerase, putative / Galactowaldenase, putative similar to UDP-galactose 4-epimerase from Arabidopsis thaliana SP|Q42605, Cyamopsis tetragonoloba GI:3021357 [AJ005082] Length = 351 Score = 28.3 bits (60), Expect = 2.3 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -1 Query: 208 GKYSQLTSSNLDSKLREDLERLKKIRAHRGMR 113 G YS + NLD+ L+R+KK+ A G R Sbjct: 26 GGYSVVVVDNLDNSSAVSLQRVKKLAAEHGER 57 >At3g07210.1 68416.m00860 expressed protein predicted using genefinder Length = 547 Score = 27.9 bits (59), Expect = 3.0 Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 10/82 (12%) Frame = +2 Query: 173 IQVGGGQLAVFTINNILLPI*EPVWYLNCLGFD----MIVIIFSTSSSVHSPARLSRSMS 340 +++G G+L V T N I L E +W GFD + +I SP S S S Sbjct: 148 LKIGEGKLVVATDNLIKLSTDEMIWSPGSAGFDVQGNLAFMICDPRKLSTSPTSTSSSSS 207 Query: 341 AFLR------TMLEYLRPTPLI 388 + L+ M+++ P P+I Sbjct: 208 SSLKKDNNKTLMMQFGIPIPVI 229 >At3g14870.1 68416.m01880 expressed protein contains Pfam profile PF04859: Plant protein of unknown function (DUF641 Length = 445 Score = 27.1 bits (57), Expect = 5.2 Identities = 20/82 (24%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Frame = +2 Query: 176 QVGGGQLAVFTINNILLPI*EPVWYLNCLGFDMIVIIFSTSSSVHSPARLSRSMSAFLRT 355 QV G+ ++ L + + VW L+CL F F +S+ +R R ++++ Sbjct: 338 QVTAGEFPETSLCTAFLEMAKRVWLLHCLAFS-----FDPEASIFQVSRGCRFSEVYMKS 392 Query: 356 MLE--YLRPTPLIAASQT*PCV 415 + E + P ++S+T P V Sbjct: 393 VSEEAFFSPEQEESSSETEPGV 414 >At5g18680.1 68418.m02217 F-box family protein / tubby family protein similar to phosphodiesterase (GI:467578) [Mus musculus]; similar to Chain A, C-Terminal Domain Of Mouse Brain Tubby Protein (GI:6730158) [Mus musculus]; contains Pfam PF00646: F-box domain and Pfam PF01167: Tub family Length = 389 Score = 26.6 bits (56), Expect = 6.9 Identities = 12/27 (44%), Positives = 19/27 (70%) Frame = +2 Query: 323 LSRSMSAFLRTMLEYLRPTPLIAASQT 403 LSRS S LR+ +L+ TPL+ +++T Sbjct: 273 LSRSQSKPLRSSSSHLKETPLVLSNKT 299 >At5g04920.1 68418.m00519 vacuolar protein sorting 36 family protein / VPS36 family protein contains Pfam PF04132: Vacuolar protein sorting 36 Length = 440 Score = 26.2 bits (55), Expect = 9.1 Identities = 8/18 (44%), Positives = 14/18 (77%) Frame = +1 Query: 163 GVLNPSWRRSAGCIYHQQ 216 G+++P + SAG +YHQ+ Sbjct: 252 GIISPVTKESAGALYHQE 269 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,759,752 Number of Sequences: 28952 Number of extensions: 169654 Number of successful extensions: 492 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 476 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 492 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 655255392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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