BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0786.Seq
(424 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g09800.1 68417.m01609 40S ribosomal protein S18 (RPS18C) 110 3e-25
At1g34030.1 68414.m04219 40S ribosomal protein S18 (RPS18B) simi... 110 3e-25
At1g22780.1 68414.m02846 40S ribosomal protein S18 (RPS18A) Matc... 110 3e-25
At5g14320.1 68418.m01674 30S ribosomal protein S13, chloroplast ... 32 0.14
At1g61710.1 68414.m06960 DC1 domain-containing protein contains ... 29 0.98
At1g49890.1 68414.m05593 expressed protein contains Pfam domain,... 29 1.3
At5g49610.1 68418.m06139 F-box family protein contains Pfam PF00... 29 1.7
At1g77750.1 68414.m09052 30S ribosomal protein S13, chloroplast,... 29 1.7
At4g10960.1 68417.m01781 UDP-glucose 4-epimerase, putative / UDP... 28 2.3
At3g07210.1 68416.m00860 expressed protein predicted using genef... 28 3.0
At3g14870.1 68416.m01880 expressed protein contains Pfam profile... 27 5.2
At5g18680.1 68418.m02217 F-box family protein / tubby family pro... 27 6.9
At5g04920.1 68418.m00519 vacuolar protein sorting 36 family prot... 26 9.1
>At4g09800.1 68417.m01609 40S ribosomal protein S18 (RPS18C)
Length = 152
Score = 110 bits (265), Expect = 3e-25
Identities = 48/58 (82%), Positives = 52/58 (89%)
Frame = -1
Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80
KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH
Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135
Score = 65.3 bits (152), Expect = 2e-11
Identities = 26/47 (55%), Positives = 40/47 (85%)
Frame = -2
Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250
+IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ +
Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78
>At1g34030.1 68414.m04219 40S ribosomal protein S18 (RPS18B) similar
to ribosomal protein S18 GI:38422 from [Homo sapiens]
Length = 152
Score = 110 bits (265), Expect = 3e-25
Identities = 48/58 (82%), Positives = 52/58 (89%)
Frame = -1
Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80
KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH
Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135
Score = 65.3 bits (152), Expect = 2e-11
Identities = 26/47 (55%), Positives = 40/47 (85%)
Frame = -2
Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250
+IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ +
Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78
>At1g22780.1 68414.m02846 40S ribosomal protein S18 (RPS18A) Match
to ribosomal S18 gene mRNA gb|Z28701, DNA gb|Z23165 from
A. thaliana. ESTs gb|T21121, gb|Z17755, gb|R64776 and
gb|R30430 come from this gene
Length = 152
Score = 110 bits (265), Expect = 3e-25
Identities = 48/58 (82%), Positives = 52/58 (89%)
Frame = -1
Query: 253 KIPDWFLNRQKDIVDGKYSQLTSSNLDSKLREDLERLKKIRAHRGMRHYWGLRVRGQH 80
KIPDWFLNRQKD DGKYSQ+ S+ LD KLR+DLERLKKIR HRG+RHYWGLRVRGQH
Sbjct: 78 KIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQH 135
Score = 65.3 bits (152), Expect = 2e-11
Identities = 26/47 (55%), Positives = 40/47 (85%)
Frame = -2
Query: 390 AIKGVGRRYSNIVLKKADIDLDKRAGECTEEEVEKIITIMSNPRQLR 250
+IKG+GRR +NIV KKAD+D++KRAGE + E++ ++TI++NPRQ +
Sbjct: 32 SIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFK 78
>At5g14320.1 68418.m01674 30S ribosomal protein S13, chloroplast
(CS13) ribosomal protein S13 precursor, chloroplast
Arabidopsis thaliana, PIR:S59594; identical to cDNA
ribosomal protein S13 GI:1515106
Length = 169
Score = 32.3 bits (70), Expect = 0.14
Identities = 13/24 (54%), Positives = 19/24 (79%)
Frame = -1
Query: 154 LERLKKIRAHRGMRHYWGLRVRGQ 83
++RLK+I+ +RG+RH GL RGQ
Sbjct: 122 IKRLKEIQCYRGVRHIQGLPCRGQ 145
>At1g61710.1 68414.m06960 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 402
Score = 29.5 bits (63), Expect = 0.98
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +1
Query: 286 FFNLFFCAFASTLVKINVGFFENNVGVPPANTFDSRIANI 405
FFNLFF A +KI +G F ++ +PP+++ + +I
Sbjct: 34 FFNLFFYAQVHENLKIELGIFSRSI-LPPSSSSLQEVLHI 72
>At1g49890.1 68414.m05593 expressed protein contains Pfam domain,
PF04484: Family of unknown function (DUF566)
Length = 659
Score = 29.1 bits (62), Expect = 1.3
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = +2
Query: 290 STSSSVHSPARLSRSMSAFLRTMLEYLRPTPLIAASQT 403
+T+++ + + S S SA LRT Y P+PL++ S T
Sbjct: 56 TTTTTTTTSSSSSSSSSAILRTSKRYPSPSPLLSRSTT 93
>At5g49610.1 68418.m06139 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain; similar to unknown protein
(gb|AAF30317.1)
Length = 359
Score = 28.7 bits (61), Expect = 1.7
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -2
Query: 111 ITGAF-VCVVSTLRLLAGEEELLVYQRRSKLNK 16
+ G F +C LA +++LVYQRRSKL K
Sbjct: 299 VPGIFPICQTGEYVFLATHKQVLVYQRRSKLWK 331
>At1g77750.1 68414.m09052 30S ribosomal protein S13, chloroplast,
putative similar to putative 30S ribosomal protein S13,
chloroplast precursor GB:P42732 [Arabidopsis thaliana]
Length = 154
Score = 28.7 bits (61), Expect = 1.7
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 178 LDSKLREDLERLKKIRAHRGMRHYWGLRVRGQ 83
L ++ +++RL ++ +RG RH GL RGQ
Sbjct: 98 LRRRVGSEIQRLVEVDCYRGSRHRHGLPCRGQ 129
>At4g10960.1 68417.m01781 UDP-glucose 4-epimerase, putative /
UDP-galactose 4-epimerase, putative / Galactowaldenase,
putative similar to UDP-galactose 4-epimerase from
Arabidopsis thaliana SP|Q42605, Cyamopsis tetragonoloba
GI:3021357 [AJ005082]
Length = 351
Score = 28.3 bits (60), Expect = 2.3
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -1
Query: 208 GKYSQLTSSNLDSKLREDLERLKKIRAHRGMR 113
G YS + NLD+ L+R+KK+ A G R
Sbjct: 26 GGYSVVVVDNLDNSSAVSLQRVKKLAAEHGER 57
>At3g07210.1 68416.m00860 expressed protein predicted using
genefinder
Length = 547
Score = 27.9 bits (59), Expect = 3.0
Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 10/82 (12%)
Frame = +2
Query: 173 IQVGGGQLAVFTINNILLPI*EPVWYLNCLGFD----MIVIIFSTSSSVHSPARLSRSMS 340
+++G G+L V T N I L E +W GFD + +I SP S S S
Sbjct: 148 LKIGEGKLVVATDNLIKLSTDEMIWSPGSAGFDVQGNLAFMICDPRKLSTSPTSTSSSSS 207
Query: 341 AFLR------TMLEYLRPTPLI 388
+ L+ M+++ P P+I
Sbjct: 208 SSLKKDNNKTLMMQFGIPIPVI 229
>At3g14870.1 68416.m01880 expressed protein contains Pfam profile
PF04859: Plant protein of unknown function (DUF641
Length = 445
Score = 27.1 bits (57), Expect = 5.2
Identities = 20/82 (24%), Positives = 38/82 (46%), Gaps = 2/82 (2%)
Frame = +2
Query: 176 QVGGGQLAVFTINNILLPI*EPVWYLNCLGFDMIVIIFSTSSSVHSPARLSRSMSAFLRT 355
QV G+ ++ L + + VW L+CL F F +S+ +R R ++++
Sbjct: 338 QVTAGEFPETSLCTAFLEMAKRVWLLHCLAFS-----FDPEASIFQVSRGCRFSEVYMKS 392
Query: 356 MLE--YLRPTPLIAASQT*PCV 415
+ E + P ++S+T P V
Sbjct: 393 VSEEAFFSPEQEESSSETEPGV 414
>At5g18680.1 68418.m02217 F-box family protein / tubby family
protein similar to phosphodiesterase (GI:467578) [Mus
musculus]; similar to Chain A, C-Terminal Domain Of
Mouse Brain Tubby Protein (GI:6730158) [Mus musculus];
contains Pfam PF00646: F-box domain and Pfam PF01167:
Tub family
Length = 389
Score = 26.6 bits (56), Expect = 6.9
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +2
Query: 323 LSRSMSAFLRTMLEYLRPTPLIAASQT 403
LSRS S LR+ +L+ TPL+ +++T
Sbjct: 273 LSRSQSKPLRSSSSHLKETPLVLSNKT 299
>At5g04920.1 68418.m00519 vacuolar protein sorting 36 family protein
/ VPS36 family protein contains Pfam PF04132: Vacuolar
protein sorting 36
Length = 440
Score = 26.2 bits (55), Expect = 9.1
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +1
Query: 163 GVLNPSWRRSAGCIYHQQ 216
G+++P + SAG +YHQ+
Sbjct: 252 GIISPVTKESAGALYHQE 269
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,759,752
Number of Sequences: 28952
Number of extensions: 169654
Number of successful extensions: 492
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 492
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 655255392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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