BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0784.Seq
(433 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P32461 Cluster: Diphthamide biosynthesis protein 2; n=3... 33 2.6
UniRef50_Q7RYG4 Cluster: Putative uncharacterized protein NCU065... 32 4.5
>UniRef50_P32461 Cluster: Diphthamide biosynthesis protein 2; n=3;
Saccharomycetales|Rep: Diphthamide biosynthesis protein
2 - Saccharomyces cerevisiae (Baker's yeast)
Length = 534
Score = 33.1 bits (72), Expect = 2.6
Identities = 17/40 (42%), Positives = 23/40 (57%)
Frame = -2
Query: 276 NFPKFFPDL*FKCHFMFNIIYIKRLSRIYD*CKDNLIYLN 157
NF + FPDL K M N + K LS++Y+ K +L Y N
Sbjct: 154 NFQRAFPDLSSKICLMANAPFSKHLSQLYNILKGDLHYTN 193
>UniRef50_Q7RYG4 Cluster: Putative uncharacterized protein
NCU06511.1; n=1; Neurospora crassa|Rep: Putative
uncharacterized protein NCU06511.1 - Neurospora crassa
Length = 797
Score = 32.3 bits (70), Expect = 4.5
Identities = 22/66 (33%), Positives = 27/66 (40%)
Frame = +1
Query: 226 KHKVALESQIWKKFGEILASTKARPPATRRWSNKIHYPYVRQDILHXRITGTRESPRVSC 405
+H ++ +IW A RPP+T SN Y Y Q H R SPR S
Sbjct: 25 RHSFDVDFKIWS-MPNASAEVGDRPPSTSSGSNDWSYSYSNQ-TAHTRNGTNGSSPRTSV 82
Query: 406 P*PCAY 423
P AY
Sbjct: 83 PPGLAY 88
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 371,918,024
Number of Sequences: 1657284
Number of extensions: 6255423
Number of successful extensions: 11850
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 11665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11848
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 21075479950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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