Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0774.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 95 2e-20
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 95 2e-20
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 1.8
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.1
At5g31685.1 68418.m03766 expressed protein ; expression supporte... 27 7.2
At5g07540.1 68418.m00863 glycine-rich protein (GRP16) oleosin; g... 27 9.5
At5g05340.1 68418.m00575 peroxidase, putative similar to peroxid... 27 9.5
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 9.5
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 9.5
At1g03050.1 68414.m00277 epsin N-terminal homology (ENTH) domain... 27 9.5
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 95.5 bits (227), Expect = 2e-20
Identities = 49/100 (49%), Positives = 58/100 (58%)
Frame = +2
Query: 260 GHQTSAESWGTGRAVARIPXVRGGGTHRSGQGAFGNMCRGGPYVRPHEALAALAPSRQPX 439
GHQTSAESWGTGRAV+RIP V GGGTHR+GQ AFGNMCRGG P +
Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124
Query: 440 TAESGLGSSRCCYWRPXARSG*RHIIEKIPELPLVVADKS 559
+ S+ P H IE +PE+PLVV+D +
Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSA 164
Score = 87.8 bits (208), Expect = 5e-18
Identities = 55/169 (32%), Positives = 69/169 (40%), Gaps = 2/169 (1%)
Frame = +3
Query: 87 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKRL 260
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 261 VTKPVPNHGVPDVLLPEFXXXXXXXXXXXXXXXXXXXXXXDRMFAPTKPWRRWHXXXXXX 440
+ + RMFAPTK WRRWH
Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124
Query: 441 XXXXXXXXXXXXTGVPXLVQARGTLLKRFPSFPWL*PTKVQEINKTKQA 587
T VP LV ARG ++ P P + + + KT A
Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 173
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 95.5 bits (227), Expect = 2e-20
Identities = 49/100 (49%), Positives = 58/100 (58%)
Frame = +2
Query: 260 GHQTSAESWGTGRAVARIPXVRGGGTHRSGQGAFGNMCRGGPYVRPHEALAALAPSRQPX 439
GHQTSAESWGTGRAV+RIP V GGGTHR+GQ AFGNMCRGG P +
Sbjct: 64 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 123
Query: 440 TAESGLGSSRCCYWRPXARSG*RHIIEKIPELPLVVADKS 559
+ S+ P H IE +PE+PLVV+D +
Sbjct: 124 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSA 163
Score = 89.8 bits (213), Expect = 1e-18
Identities = 55/172 (31%), Positives = 73/172 (42%), Gaps = 2/172 (1%)
Frame = +3
Query: 78 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 251
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 252 KRLVTKPVPNHGVPDVLLPEFXXXXXXXXXXXXXXXXXXXXXXDRMFAPTKPWRRWHXXX 431
K+ + + RMFAPTK WRRWH
Sbjct: 61 KKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120
Query: 432 XXXXXXXXXXXXXXXTGVPXLVQARGTLLKRFPSFPWL*PTKVQEINKTKQA 587
T VP LV ARG ++ P P + + + KT A
Sbjct: 121 NVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 172
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 102 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 248
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.1
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 153 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 248
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 153 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 248
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g31685.1 68418.m03766 expressed protein ; expression supported
by MPSS
Length = 519
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +2
Query: 380 GPYVRPHEALAALAPSRQ 433
GPY PH L + PSRQ
Sbjct: 449 GPYTEPHRKLEEIKPSRQ 466
>At5g07540.1 68418.m00863 glycine-rich protein (GRP16) oleosin;
glycine-rich protein 16 (GRP16) PMID:11431566
Length = 244
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/75 (26%), Positives = 25/75 (33%), Gaps = 1/75 (1%)
Frame = +2
Query: 260 GHQTSAESWGTGRAVARIPX-VRGGGTHRSGQGAFGNMCRGGPYVRPHEALAALAPSRQP 436
G A G G A P GGG + GA G+ G P +P A +
Sbjct: 170 GASGGASGGGPGGASGGGPGGASGGGPGGASGGASGDKPEGAPGDKPGGAWGGKPGKKPG 229
Query: 437 XTAESGLGSSRCCYW 481
E G R +W
Sbjct: 230 HKPEGARGGKRLAWW 244
>At5g05340.1 68418.m00575 peroxidase, putative similar to peroxidase
[Nicotiana tabacum] gi|5381253|dbj|BAA82306; similar to
Peroxidase P7 [Brassica rapa (Turnip)] SWISS-PROT:P00434
Length = 324
Score = 27.1 bits (57), Expect = 9.5
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +2
Query: 77 NESIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGPGQ*CSRF 214
N +I +PT S+ G S V + GAHT G + C+ F
Sbjct: 163 NSNIPAPTSSLSQLISSFSAVGLSTRDMVALSGAHTIGQSR-CTNF 207
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 323 RGGGTHRSGQGAFGNMCRGGPYVRP 397
RGG H+ G+G +G GGP P
Sbjct: 85 RGGPPHQGGRGGYGGGRGGGPSSGP 109
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 323 RGGGTHRSGQGAFGNMCRGGPYVRP 397
RGG H+ G+G +G GGP P
Sbjct: 85 RGGPPHQGGRGGYGGGRGGGPSSGP 109
>At1g03050.1 68414.m00277 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to CLATHRIN
COAT ASSEMBLY PROTEIN AP180 - Mus musculus,
SWISSPROT:Q61548
Length = 599
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 141 PAPSHSSLNTPTLKVGLPIDSFRYFSEAIPPKY 43
PAPS ++ N + +P+D F E PP Y
Sbjct: 519 PAPSTANGNAGNINSPVPMDPFAASLEVAPPAY 551
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,496,945
Number of Sequences: 28952
Number of extensions: 250019
Number of successful extensions: 726
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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