Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0763.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp... 71 4e-13
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 70 1e-12
At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ... 31 0.44
At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ... 31 0.44
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 31 0.44
At5g34780.1 68418.m04048 dehydrogenase E1 component family prote... 29 1.8
At1g54350.1 68414.m06196 ABC transporter family protein similar ... 29 1.8
At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive eff... 29 2.4
At5g05970.1 68418.m00661 transducin family protein / WD-40 repea... 29 3.1
At1g21810.1 68414.m02729 expressed protein 28 4.1
At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containi... 28 5.4
At1g66210.1 68414.m07515 subtilase family protein contains simil... 28 5.4
At1g05570.1 68414.m00575 callose synthase 1 (CALS1) / 1,3-beta-g... 28 5.4
At5g64580.1 68418.m08116 AAA-type ATPase family protein similar ... 27 7.2
At4g25580.1 68417.m03686 stress-responsive protein-related conta... 27 7.2
At1g76780.1 68414.m08935 expressed protein ; expression supporte... 27 7.2
At1g70620.2 68414.m08137 cyclin-related contains weak similarity... 27 7.2
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 27 7.2
At1g13330.1 68414.m01547 expressed protein similar to nuclear re... 27 7.2
At4g32130.1 68417.m04571 expressed protein 27 9.5
At1g71140.1 68414.m08209 MATE efflux family protein similar to r... 27 9.5
At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin ... 27 9.5
>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial, putative similar to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 393
Score = 71.3 bits (167), Expect = 4e-13
Identities = 34/79 (43%), Positives = 50/79 (63%)
Frame = -2
Query: 507 DVGPWYVDRTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQS 328
D G Y RTRDE+ VRQ RDPI ++ +L H++ T +LKD++ ++RKEVD+A Q+
Sbjct: 299 DPGSTY--RTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQA 356
Query: 327 KTEPEVGIEELSADIYYKN 271
K P EL ++Y K+
Sbjct: 357 KESPIPDASELFTNMYVKD 375
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -3
Query: 554 GPLVMEMETVPLLWSSMSDPG 492
GP+++EM+T SMSDPG
Sbjct: 281 GPIILEMDTYRYHGHSMSDPG 301
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 70.1 bits (164), Expect = 1e-12
Identities = 35/78 (44%), Positives = 50/78 (64%)
Frame = -2
Query: 507 DVGPWYVDRTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQS 328
D G Y RTRDE+ VRQ RDPI K+ +L+H+L T +LKD++ ++RKEVD+A ++
Sbjct: 295 DPGSTY--RTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKA 352
Query: 327 KTEPEVGIEELSADIYYK 274
K P EL ++Y K
Sbjct: 353 KDCPMPEPSELFTNVYVK 370
Score = 29.9 bits (64), Expect = 1.3
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = -3
Query: 557 KGPLVMEMETVPLLWSSMSDPG 492
KGP+++EM+T SMSDPG
Sbjct: 276 KGPIILEMDTYRYHGHSMSDPG 297
>At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 401
Score = 31.5 bits (68), Expect = 0.44
Identities = 18/91 (19%), Positives = 48/91 (52%)
Frame = -2
Query: 483 RTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEVGI 304
R+ E++ + R+P++ F+ I ++ + D+ ++++KE+ EA + ++ + +
Sbjct: 303 RSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNL 362
Query: 303 EELSADIYYKNLEPFVLASTRLPRSNTLRSN 211
+ + +D+Y ++ P L L T+ S+
Sbjct: 363 QNMFSDVY--DVPPSNLREQELLVRQTINSH 391
>At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 31.5 bits (68), Expect = 0.44
Identities = 18/91 (19%), Positives = 48/91 (52%)
Frame = -2
Query: 483 RTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEVGI 304
R+ E++ + R+P++ F+ I ++ + D+ ++++KE+ EA + ++ + +
Sbjct: 374 RSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNL 433
Query: 303 EELSADIYYKNLEPFVLASTRLPRSNTLRSN 211
+ + +D+Y ++ P L L T+ S+
Sbjct: 434 QNMFSDVY--DVPPSNLREQELLVRQTINSH 462
>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 31.5 bits (68), Expect = 0.44
Identities = 18/76 (23%), Positives = 41/76 (53%), Gaps = 3/76 (3%)
Frame = -2
Query: 483 RTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEVGI 304
R DE+Q + +R+P+ F++ + ++ + + + + RK++ +A + ++ + +
Sbjct: 374 RAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPL 433
Query: 303 EELSADIY---YKNLE 265
EL D+Y KNLE
Sbjct: 434 TELFNDVYDVKPKNLE 449
>At5g34780.1 68418.m04048 dehydrogenase E1 component family protein
similar to SP|P50136 2-oxoisovalerate dehydrogenase
alpha subunit, mitochondrial precursor (EC 1.2.4.4)
(Branched-chain alpha-keto acid dehydrogenase component
alpha chain) {Mus musculus}; contains Pfam profile
PF00676: Dehydrogenase E1 component
Length = 365
Score = 29.5 bits (63), Expect = 1.8
Identities = 17/76 (22%), Positives = 40/76 (52%), Gaps = 3/76 (3%)
Frame = -2
Query: 483 RTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEVGI 304
R DE+Q + +R+ + F++ + ++ + + + + RK++ +A + ++ + +
Sbjct: 144 RAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPL 203
Query: 303 EELSADIY---YKNLE 265
EL D+Y KNLE
Sbjct: 204 TELFNDVYDVKPKNLE 219
>At1g54350.1 68414.m06196 ABC transporter family protein similar to
hypothetical ABC transporter ATP-binding protein
GI:9955395 from [Microcystis aeruginosa]
Length = 706
Score = 29.5 bits (63), Expect = 1.8
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = -2
Query: 450 TRDPITSFKEKILNHELV-TPDQLKDIDAKVRKEVDEATKQSKTEPEVGIEELSADIYYK 274
T D I + +N L+ T +K + R E++E T Q+ T + LSAD+Y K
Sbjct: 398 TVDEIELTYQSEMNSSLLDTNGSIKSQPNQKRLEIEELTLQTPTNGTTLVHNLSADVYDK 457
Query: 273 N 271
+
Sbjct: 458 D 458
>At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1311
Score = 29.1 bits (62), Expect = 2.4
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +1
Query: 427 ERSDGVPGLPHLLYLVPRPIDIPGSDIDDQSN 522
E++ GVPG H++Y +PIDIP I +++
Sbjct: 272 EQAGGVPG--HVVYRDQKPIDIPNGGIHPETS 301
>At5g05970.1 68418.m00661 transducin family protein / WD-40 repeat
family protein contains similarity to regulatory protein
Nedd1; contains Pfam PF00400: WD domain, G-beta repeat
(6 copies, 2 weak)|19804256|gb|AV785466.1|AV785466
Length = 781
Score = 28.7 bits (61), Expect = 3.1
Identities = 14/56 (25%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = -2
Query: 426 KEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPE--VGIEELSADIYYKNLE 265
+E++LNH L P+ + +A ++ KQS+T+ + +G + ++ + LE
Sbjct: 662 REEVLNHLLARPETVVATEAGAMPLMNGGLKQSQTDQQQVMGSSNFTLQLFQRTLE 717
>At1g21810.1 68414.m02729 expressed protein
Length = 628
Score = 28.3 bits (60), Expect = 4.1
Identities = 17/59 (28%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
Frame = -2
Query: 462 EVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEAT--KQSKTEPEVGIEELS 292
E R TR KE+I++ L ++ + + ++R+E++E T K+ K EP++ E+++
Sbjct: 448 ESRVTRMEAEVRKERIVSDGL--KEKCETFEEELRREIEEKTMIKREKVEPKIKQEDIA 504
>At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 603
Score = 27.9 bits (59), Expect = 5.4
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -2
Query: 492 YVDRTRDEVQEVRQTRDP-ITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSK 325
YVD R +++ + + P +S + + HE + D +K K+ + +DE K+ K
Sbjct: 451 YVDSLRKVMKDRKAVKVPGCSSIEVNNVVHEFFSGDGVKSATTKLHRALDEMVKELK 507
>At1g66210.1 68414.m07515 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 759
Score = 27.9 bits (59), Expect = 5.4
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -2
Query: 447 RDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEVGIE 301
R I S+K N E TPD +K ID +R VD + +E V E
Sbjct: 261 RARIASYKVCWNNEECFTPDIVKAIDHAIRDGVDVLSLSLGSEVPVDFE 309
>At1g05570.1 68414.m00575 callose synthase 1 (CALS1) / 1,3-beta-glucan
synthase 1 nearly identical to callose synthase 1
catalytic subunit [Arabidopsis thaliana] GI:13649388
Length = 1922
Score = 27.9 bits (59), Expect = 5.4
Identities = 13/47 (27%), Positives = 28/47 (59%)
Frame = +1
Query: 286 IGGQLFNTDFRFRLRLFSGLVYFFTYLGVDILELIGRDELVIQDLLL 426
+G + F+T+F+ R+ GLV F T++ + ++ + + I+DL +
Sbjct: 1782 VGRRRFSTNFQLLFRIIKGLV-FLTFVAI-LITFLALPLITIKDLFI 1826
>At5g64580.1 68418.m08116 AAA-type ATPase family protein similar to
zinc dependent protease [Arabidopsis thaliana]
GI:7650138; contains Pfam profile PF00004: ATPase AAA
family
Length = 855
Score = 27.5 bits (58), Expect = 7.2
Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = -2
Query: 486 DRTRDEVQEVRQTRDPITSFK-EKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEV 310
D + +QEV + + T + + +LN + + KD+D R+E+ EA K+ K E
Sbjct: 510 DEKEELLQEVAENTEDFTGAELQNVLNEAGILTAR-KDLDYIGREELLEALKRQKGTFET 568
Query: 309 GIEE 298
G E+
Sbjct: 569 GQED 572
>At4g25580.1 68417.m03686 stress-responsive protein-related contains
weak similarity to Low-temperature-induced 65 kDa
protein (Desiccation-responsive protein 29B)
(Swiss-Prot:Q04980) [Arabidopsis thaliana]
Length = 626
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -1
Query: 481 DEGRGTGGEADQGPHHFVQGEDLESRARHARSAQGYRRQGT*RSRRGH 338
D G G E+ + F G+DL +R + ++ QG+ +G R H
Sbjct: 332 DYGSRLGKESPERSDEFDLGKDLPTRTQGIQNPQGFDSRGQRRGEEMH 379
>At1g76780.1 68414.m08935 expressed protein ; expression supported by
MPSS
Length = 1871
Score = 27.5 bits (58), Expect = 7.2
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = -2
Query: 489 VDRTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATKQSKTEPEV 310
+ +T+DE+++ R+ + E HE + Q +DI+ K + ++ KQ+K + +
Sbjct: 1481 IQQTKDELEKPRKPSE----ISENHNIHEFMDSSQSQDIEEKGSDQAEKYAKQNKIQEVM 1536
Query: 309 GIEE 298
E+
Sbjct: 1537 NDED 1540
>At1g70620.2 68414.m08137 cyclin-related contains weak similarity to
Swiss-Prot:P35662 cylicin I (Multiple-band polypeptide
I) [Bos taurus]
Length = 884
Score = 27.5 bits (58), Expect = 7.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2
Query: 426 KEKILNHELVTPDQLKDIDAKVRKEVDEATKQ 331
+E+++ H+L TP +LK + RKE T Q
Sbjct: 245 QERLVTHKLCTPSELKLVTLFARKEESGNTTQ 276
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 27.5 bits (58), Expect = 7.2
Identities = 20/85 (23%), Positives = 38/85 (44%), Gaps = 1/85 (1%)
Frame = -2
Query: 489 VDRTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDEATK-QSKTEPE 313
+ + ++ + + IT E++ E Q++++ VDEATK QS +
Sbjct: 622 LSKVKESLVDKETKLQSITQEAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQES 681
Query: 312 VGIEELSADIYYKNLEPFVLASTRL 238
++E A Y K +E +A+ L
Sbjct: 682 EDLKEKEAG-YLKKIEELSVANESL 705
>At1g13330.1 68414.m01547 expressed protein similar to nuclear
receptor coactivator GT198 (GI:16506273) {Rattus
norvegicus}; similar to TBP-1 interacting protein
(GI:7328534) [Homo sapiens]
Length = 226
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/47 (34%), Positives = 31/47 (65%)
Frame = -2
Query: 483 RTRDEVQEVRQTRDPITSFKEKILNHELVTPDQLKDIDAKVRKEVDE 343
+ ++++QE ++T + S + K L L T +++++ DAK+RKEV E
Sbjct: 90 KLQEQLQEKKKTISDVES-EIKSLQSNL-TLEEIQEKDAKLRKEVKE 134
>At4g32130.1 68417.m04571 expressed protein
Length = 202
Score = 27.1 bits (57), Expect = 9.5
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -2
Query: 378 DIDAKVRKEVDEA-TKQSKTEPEVGIEELSADIYYKNLEPFVLAS 247
D+ A+ R +V T+ ++ E+ +E L A+ YY+ EPF + S
Sbjct: 103 DVSARHRGKVQATLTETRRSLTELVLEPLRAEQYYEMREPFSVMS 147
>At1g71140.1 68414.m08209 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 485
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +1
Query: 343 LVYFFTYLGVDILELIGRDELVIQD 417
L +TY+G DIL LIG+D +V Q+
Sbjct: 122 LSLLWTYIG-DILSLIGQDAMVAQE 145
>At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin
family protein very low similarity to Ser Thr protein
kinase GI:2598067 from (Zea mays); contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 919
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = -1
Query: 274 ELGTLRPGIHPAAPLKHLEVQPR 206
+LG PG+ P P KH +PR
Sbjct: 210 QLGLATPGLRPEFPYKHFLARPR 232
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,314,015
Number of Sequences: 28952
Number of extensions: 249327
Number of successful extensions: 878
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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