Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0761.Seq
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin fa... 32 0.38
At1g26150.1 68414.m03192 protein kinase family protein similar t... 31 0.66
At1g13050.1 68414.m01513 expressed protein 31 0.87
At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family... 29 2.7
At5g07760.1 68418.m00888 formin homology 2 domain-containing pro... 29 3.5
At4g00890.1 68417.m00120 proline-rich family protein contains pr... 29 3.5
At5g48360.1 68418.m05975 formin homology 2 domain-containing pro... 28 6.1
At5g02400.1 68418.m00163 protein phosphatase 2C family protein /... 28 6.1
At5g61090.1 68418.m07665 proline-rich family protein contains pr... 27 8.1
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 27 8.1
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 27 8.1
>At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245, SP|Q03211 Pistil-specific
extensin-like protein precursor (PELP) {Nicotiana
tabacum}; contains Pfam profile PF01190: Pollen proteins
Ole e I family
Length = 239
Score = 31.9 bits (69), Expect = 0.38
Identities = 23/64 (35%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = -2
Query: 500 GNQDPVTKALPDSHPQPIRRAPTVP--AAPIPPQARPGQAQPLQAVLLPRAVQVHR--TL 333
G+ DP +LP P P + PT+P API A P P++ LP A + TL
Sbjct: 31 GSSDPF-HSLPQHLPLPPIKLPTLPPAKAPIKLPAYPPAKAPIKLPTLPPAKAPIKLPTL 89
Query: 332 PPLR 321
PP++
Sbjct: 90 PPIK 93
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 31.1 bits (67), Expect = 0.66
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -2
Query: 497 NQDPVTKALPDSHPQPIRRAPTVPAAPIPPQARPGQAQPL-QAVLLPRAVQVHRTLPP 327
N P K +P SH P R P+ PA+ IPP R + P + P + H + PP
Sbjct: 165 NPLPPPKLVPPSHSPP-RHLPSPPASEIPPPPRHLPSPPASERPSTPPSDSEHPSPPP 221
Score = 27.9 bits (59), Expect = 6.1
Identities = 22/65 (33%), Positives = 27/65 (41%), Gaps = 4/65 (6%)
Frame = -2
Query: 491 DPVTKALPDSH---PQPIRRAPTVP-AAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPL 324
+PV+ P+S P P PT P +P PP P P LP LPP
Sbjct: 113 NPVSSPPPESSPPPPPPTEAPPTTPITSPSPPTNPP--PPPESPPSLPAPDPPSNPLPPP 170
Query: 323 RLVFP 309
+LV P
Sbjct: 171 KLVPP 175
>At1g13050.1 68414.m01513 expressed protein
Length = 317
Score = 30.7 bits (66), Expect = 0.87
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -2
Query: 470 PDSHPQPIRRAPTVPAAPIPPQARPGQAQPLQ 375
P S P P+R P P+PP+ P A+PLQ
Sbjct: 68 PSSRPLPLR-----PEEPLPPRHNPNSARPLQ 94
>At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family
protein identical to proline-rich protein 2 [Arabidopsis
thaliana] gi|7620011|gb|AAF64549
Length = 321
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
Frame = -2
Query: 458 PQPIRRAPTV-PAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPL 324
P PI + P V P P PP+ +P + P V +T PPL
Sbjct: 219 PVPIYKPPVVIPKKPCPPKIHKPIYKPPVPIYKPPVVIPKKTFPPL 264
>At5g07760.1 68418.m00888 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 853
Score = 28.7 bits (61), Expect = 3.5
Identities = 18/52 (34%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
Frame = -2
Query: 458 PQPIRRAPTVPAAPIPPQARPG-QAQPLQAVLLPRAVQVHRTLPPLRLVFPG 306
P P R +P P PP P A+ L P V+ LPP L+F G
Sbjct: 58 PPPAMRRRVLPRPPPPPPPLPMFDAEVLCCCYPPTRVRREAPLPPPPLIFVG 109
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/48 (31%), Positives = 18/48 (37%)
Frame = -2
Query: 470 PDSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPP 327
P P P+RR +P P PP R P + R V PP
Sbjct: 27 PPPPPPPMRRRAPLPPPPPPPMRRRAPLPPPPPPAMRRRVLPRPPPPP 74
>At4g00890.1 68417.m00120 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 431
Score = 28.7 bits (61), Expect = 3.5
Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = -2
Query: 494 QDPVTKALPDS---HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTL 333
Q+ ++++ P S HPQ ++P +P+ P+++ L+ + V+ HR L
Sbjct: 36 QETLSQSPPQSPLFHPQSPPEPKSLPLSPLSPKSKEEPESQTMTPLMSKHVKTHRNL 92
>At5g48360.1 68418.m05975 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 782
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 6/51 (11%)
Frame = -2
Query: 452 PIRRAPTVPAAPIPPQAR------PGQAQPLQAVLLPRAVQVHRTLPPLRL 318
P+ +P P P+PP P + A++LPR+ + H T P L L
Sbjct: 54 PLESSPPSPPPPLPPTPPTTFAVFPTFPANISALVLPRSSKPHHTSPTLLL 104
>At5g02400.1 68418.m00163 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2c
(GI:3608412) [Mesembryanthemum crystallinum]; contains
Pfam PF00481 : Protein phosphatase 2C domain
Length = 674
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = +2
Query: 146 NLKHYKEYSKSCLVVLNTDILTEMVRNIEYVFEAKAF 256
+L H+K S+ ++L++D L E N E +FE ++F
Sbjct: 575 SLCHHKLTSRDKFLILSSDGLYEYFSNQEAIFEVESF 611
>At5g61090.1 68418.m07665 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
contains similarity to vegetative cell wall protein gp1
[Chlamydomonas reinhardtii] gi|12018147|gb|AAG45420;
common family members: At4g18570, At3g25690, At4g04980
[Arabidopsis thaliana]
Length = 344
Score = 27.5 bits (58), Expect = 8.1
Identities = 22/59 (37%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
Frame = -2
Query: 482 TKALPDSHPQ-PIRRAPT-VPAAPIPPQARPGQAQPLQAVLLP---RAVQVHRTLPPLR 321
T P S P PI P VPA P P RP A+P + P R + +TL P R
Sbjct: 100 TPEAPRSVPACPIPEIPRPVPARPTPETPRPVTARPTPEIPRPVPARPISEVQTLVPTR 158
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 27.5 bits (58), Expect = 8.1
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = -2
Query: 461 HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQV--HRTLPP 327
HPQ + P +P +PP + PL LPR +Q+ H +PP
Sbjct: 580 HPQQMLPMPNMPHHQLPPSSH----MPLHPHHLPRPMQMPPHGHMPP 622
>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 340
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/56 (26%), Positives = 26/56 (46%)
Frame = -2
Query: 497 NQDPVTKALPDSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLP 330
+Q P T L P P + ++P+ P PP + A+P+ + + + RT P
Sbjct: 185 HQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSSSFHAEPIPPTVGTSSTKRRRTTP 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,610,049
Number of Sequences: 28952
Number of extensions: 250776
Number of successful extensions: 869
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 727
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 858
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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