Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0755.Seq
(548 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 124 4e-29
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 121 4e-28
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 120 8e-28
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 103 7e-23
SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) 77 1e-14
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 68 6e-12
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 1e-06
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 48 4e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 43 2e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 40 0.002
SB_29854| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.20
SB_27085| Best HMM Match : Filamin (HMM E-Value=1.5) 31 0.82
SB_41920| Best HMM Match : Filamin (HMM E-Value=0.00015) 30 1.4
SB_25455| Best HMM Match : DUF948 (HMM E-Value=0.11) 28 4.4
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 124 bits (300), Expect = 4e-29
Identities = 57/81 (70%), Positives = 67/81 (82%)
Frame = +1
Query: 1 AIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVN 180
AIAYGLDKK ERNVLI+DLGGGTFDVS+LTIEDGIFEVKST GDTHLGGEDFDN+MV+
Sbjct: 180 AIAYGLDKKVGVERNVLIYDLGGGTFDVSVLTIEDGIFEVKSTAGDTHLGGEDFDNKMVD 239
Query: 181 HFVQELRGNTKRTSLPTRELL 243
HFV++ + K+ P + +
Sbjct: 240 HFVKQFKQKYKKDITPNKRAM 260
Score = 117 bits (282), Expect = 6e-27
Identities = 58/73 (79%), Positives = 65/73 (89%), Gaps = 2/73 (2%)
Frame = +2
Query: 254 SACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDA 433
+ACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELN DLF+ T EPVE+++RD+
Sbjct: 265 TACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNQDLFKKTTEPVEQAIRDS 324
Query: 434 KM--DKAQIHDIV 466
K+ K IHDIV
Sbjct: 325 KIPGGKESIHDIV 337
Score = 34.7 bits (76), Expect = 0.050
Identities = 14/19 (73%), Positives = 17/19 (89%)
Frame = +3
Query: 198 KRKYKKDLATNKRALRRLR 254
K+KYKKD+ NKRA+RRLR
Sbjct: 246 KQKYKKDITPNKRAMRRLR 264
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 121 bits (291), Expect = 4e-28
Identities = 57/81 (70%), Positives = 65/81 (80%)
Frame = +1
Query: 1 AIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVN 180
AIAYGLDKK ERNVLIFDLGGGTFDVS+L+IEDGIFEVKST GDTHLGGEDFDN MV+
Sbjct: 273 AIAYGLDKKVGQERNVLIFDLGGGTFDVSVLSIEDGIFEVKSTHGDTHLGGEDFDNNMVD 332
Query: 181 HFVQELRGNTKRTSLPTRELL 243
FV++ + K+ P + L
Sbjct: 333 FFVKQFKQKYKKDITPNKRAL 353
Score = 112 bits (270), Expect = 2e-25
Identities = 53/72 (73%), Positives = 67/72 (93%), Gaps = 1/72 (1%)
Frame = +2
Query: 254 SACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDA 433
+ACERAKRTLSSSTQASIEIDSL++GIDFYTSITRARFEE+NA LF+ T+EPVEK+++D+
Sbjct: 358 TACERAKRTLSSSTQASIEIDSLYDGIDFYTSITRARFEEMNAHLFKKTLEPVEKAIKDS 417
Query: 434 KMD-KAQIHDIV 466
K++ K +I +IV
Sbjct: 418 KLESKEKIDEIV 429
Score = 35.5 bits (78), Expect = 0.029
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3
Query: 198 KRKYKKDLATNKRALRRLR 254
K+KYKKD+ NKRALRRLR
Sbjct: 339 KQKYKKDITPNKRALRRLR 357
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 120 bits (289), Expect = 8e-28
Identities = 56/73 (76%), Positives = 64/73 (87%), Gaps = 1/73 (1%)
Frame = +1
Query: 1 AIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIEDG-IFEVKSTXGDTHLGGEDFDNRMV 177
A+AYGLDK +GE+NVLIFDLGGGTFDVSILTI+DG +FEVKST GDTHLGGEDFDNR+V
Sbjct: 180 ALAYGLDKSLSGEKNVLIFDLGGGTFDVSILTIDDGSLFEVKSTAGDTHLGGEDFDNRLV 239
Query: 178 NHFVQELRGNTKR 216
NHFV E + K+
Sbjct: 240 NHFVAEFKRKYKK 252
Score = 109 bits (262), Expect = 1e-24
Identities = 50/71 (70%), Positives = 62/71 (87%)
Frame = +2
Query: 254 SACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDA 433
+ACERAKRTLSSST+ASIE+DSLFEGIDFYT I+RARFE+L +DLF S ++PV K+L DA
Sbjct: 266 TACERAKRTLSSSTEASIEVDSLFEGIDFYTKISRARFEDLCSDLFLSCLDPVNKALSDA 325
Query: 434 KMDKAQIHDIV 466
K K+ IH++V
Sbjct: 326 KFSKSDIHEVV 336
Score = 34.7 bits (76), Expect = 0.050
Identities = 14/19 (73%), Positives = 17/19 (89%)
Frame = +3
Query: 198 KRKYKKDLATNKRALRRLR 254
KRKYKKD++ N RA+RRLR
Sbjct: 247 KRKYKKDMSKNSRAMRRLR 265
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 103 bits (248), Expect = 7e-23
Identities = 44/64 (68%), Positives = 57/64 (89%)
Frame = +1
Query: 1 AIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVN 180
AIAYG+DKK GE+N+L+FDLGGGTFDVS+LTI++G+FEV +T GDTHLGGEDFD ++
Sbjct: 878 AIAYGMDKK-EGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQNVME 936
Query: 181 HFVQ 192
HF++
Sbjct: 937 HFIK 940
Score = 88.6 bits (210), Expect = 3e-18
Identities = 41/68 (60%), Positives = 54/68 (79%)
Frame = +2
Query: 263 ERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMD 442
E+AKR LS+ QA +EI+S FEG DF +TRARFEELNA LF+ST++PV+K L DA +
Sbjct: 965 EKAKRALSTQHQARVEIESFFEGEDFSEMLTRARFEELNAKLFKSTLKPVQKVLEDADLK 1024
Query: 443 KAQIHDIV 466
K++IH+IV
Sbjct: 1025 KSEIHEIV 1032
>SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 766
Score = 76.6 bits (180), Expect = 1e-14
Identities = 30/52 (57%), Positives = 42/52 (80%)
Frame = +1
Query: 46 VLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVNHFVQELR 201
+ ++DLGGGTFD+SIL I+ G+FEVK+T GDT+LGGEDFDN ++ + E +
Sbjct: 2 IAVYDLGGGTFDISILEIQKGVFEVKATNGDTYLGGEDFDNTLLKFLIAEFK 53
Score = 46.0 bits (104), Expect = 2e-05
Identities = 26/74 (35%), Positives = 42/74 (56%), Gaps = 4/74 (5%)
Frame = +2
Query: 257 ACERAKRTLSSSTQASIEIDSLFEGID----FYTSITRARFEELNADLFRSTMEPVEKSL 424
A E+AK LSSS Q I + + ++R++FE L ADL T+ P +K L
Sbjct: 73 AAEKAKIELSSSVQTDINLPYITVDASGPKHLNMKLSRSKFESLVADLINRTVGPCKKCL 132
Query: 425 RDAKMDKAQIHDIV 466
+DA+++K +I D++
Sbjct: 133 QDAEINKGEIGDVL 146
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 67.7 bits (158), Expect = 6e-12
Identities = 31/64 (48%), Positives = 44/64 (68%)
Frame = +1
Query: 1 AIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVN 180
A+AYGL KK G NV++ DLGGGT DVS+L I+ G+F + G+ LGG+DF+ R +
Sbjct: 210 ALAYGLHKK-EGVANVMVVDLGGGTLDVSLLNIQGGMFVTMAMAGNNRLGGQDFNARFMQ 268
Query: 181 HFVQ 192
+ +Q
Sbjct: 269 YLLQ 272
Score = 44.4 bits (100), Expect = 6e-05
Identities = 22/73 (30%), Positives = 44/73 (60%), Gaps = 5/73 (6%)
Frame = +2
Query: 263 ERAKRTLSSSTQASIEI--DSLFEG---IDFYTSITRARFEELNADLFRSTMEPVEKSLR 427
E AK L+ + + +I++ SL EG + F +I+R FE +N DLF+ ++P+ + L
Sbjct: 296 EAAKINLTRTNEVTIKLMLPSLSEGKKIVKFKETISRKMFETINEDLFKKVLQPIRRVLA 355
Query: 428 DAKMDKAQIHDIV 466
+ ++ K ++ ++V
Sbjct: 356 EVELPKEEVDEVV 368
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 50.0 bits (114), Expect = 1e-06
Identities = 23/67 (34%), Positives = 37/67 (55%)
Frame = +2
Query: 266 RAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 445
R K+ LS++ + +I+ +F+G DF +TR EE+ DLF PV ++L+ A M
Sbjct: 253 RVKQVLSANNEIFAQIEGVFDGKDFRVKVTREELEEMCQDLFDRVAGPVNRALKSASMTM 312
Query: 446 AQIHDIV 466
I +V
Sbjct: 313 NDIDSVV 319
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 48.4 bits (110), Expect = 4e-06
Identities = 27/74 (36%), Positives = 39/74 (52%), Gaps = 3/74 (4%)
Frame = +1
Query: 1 AIAYGLDKKGTGE---RNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNR 171
++AYG+ K+ RNV+ D+G + V I G +V ST + +LGG DFD
Sbjct: 237 SLAYGIYKQDLPTDKPRNVVFVDIGHSSLQVCITAFLKGQLKVLSTAVEPNLGGRDFDYV 296
Query: 172 MVNHFVQELRGNTK 213
+V HF QE + K
Sbjct: 297 LVEHFAQEFKTKYK 310
Score = 41.1 bits (92), Expect = 6e-04
Identities = 23/70 (32%), Positives = 40/70 (57%), Gaps = 1/70 (1%)
Frame = +2
Query: 260 CERAKRTLSSST-QASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAK 436
CE+ K+ +S++T + I I+ E D + + RA+FEEL ADL + P+ +L A+
Sbjct: 327 CEKLKKLMSANTSEIPINIECFMEDKDVHGRMKRAQFEELAADLLKLVEAPLRSAL--AQ 384
Query: 437 MDKAQIHDIV 466
K + ++V
Sbjct: 385 SGKYSVKNVV 394
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 42.7 bits (96), Expect = 2e-04
Identities = 17/55 (30%), Positives = 36/55 (65%)
Frame = +1
Query: 37 ERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDFDNRMVNHFVQELR 201
+ VL++ LGG + DV++L++ +G+++V +T D LGG +FD +++ + +
Sbjct: 153 DSTVLVYRLGGASHDVTLLSVINGMYKVLATEYDGALGGRNFDEVLLDLLANDFK 207
Score = 33.1 bits (72), Expect = 0.15
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +2
Query: 218 PRYQQESS*AFASACERAKRTLSSSTQASIEIDSLFEGIDFYTSITR 358
P + S ++ E+ K LS+ A+ +DSL EGIDF ++R
Sbjct: 219 PLTNKRSKTKLQTSAEQCKNILSTLESANCSVDSLCEGIDFQGQVSR 265
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/39 (41%), Positives = 29/39 (74%)
Frame = +2
Query: 350 ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIV 466
++R++FE L ADL T+ P +K L+DA+++K +I D++
Sbjct: 53 LSRSKFESLVADLINRTVGPCKKCLQDAEINKGEIGDVL 91
>SB_29854| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 3235
Score = 32.7 bits (71), Expect = 0.20
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +1
Query: 25 KGTGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDF 162
+G E + D G GT VS + +E+G +++ D H+ G F
Sbjct: 1515 EGPSEAKLTCQDNGDGTCSVSYIPVEEGDYDIHIKFADEHIPGSPF 1560
Score = 31.5 bits (68), Expect = 0.47
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 43 NVLIFDLGGGTFDVSILTIEDGIFEVKSTXGDTHLGGEDF 162
+ LI D G+F VS E+GI ++ GD H+ G F
Sbjct: 1428 DTLITDNQDGSFGVSYTPFEEGIHDLAVKFGDDHIPGSPF 1467
>SB_27085| Best HMM Match : Filamin (HMM E-Value=1.5)
Length = 634
Score = 30.7 bits (66), Expect = 0.82
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +1
Query: 46 VLIFDLGGGTFDVSILTIEDGIFEVK 123
V I+DLG GT++++IL E G++ ++
Sbjct: 319 VPIYDLGNGTYEMAILFHEPGVYSIR 344
>SB_41920| Best HMM Match : Filamin (HMM E-Value=0.00015)
Length = 600
Score = 29.9 bits (64), Expect = 1.4
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = +1
Query: 52 IFDLGGGTFDVSILTIEDGIFEV 120
+FDLG G+++V L +E G++ V
Sbjct: 311 VFDLGNGSYEVLFLVMEPGVYRV 333
>SB_25455| Best HMM Match : DUF948 (HMM E-Value=0.11)
Length = 260
Score = 28.3 bits (60), Expect = 4.4
Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = +2
Query: 311 IDSLFEGIDFYTSITRARFEELNAD--LFRSTMEPVEKSLRD 430
+DSL +DF T+ T A F +LN R T+ + SL +
Sbjct: 168 LDSLAVRVDFTTNTTNALFTQLNTTDAAIRDTLSQINSSLSE 209
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,141,109
Number of Sequences: 59808
Number of extensions: 340186
Number of successful extensions: 3556
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 3454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3554
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1264269032
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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