Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0749.Seq
(797 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 177 1e-46
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 175 3e-46
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 173 1e-45
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 170 1e-44
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.4
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.3
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.3
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.3
DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex det... 22 5.7
AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein ... 22 5.7
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 177 bits (431), Expect = 1e-46
Identities = 89/119 (74%), Positives = 97/119 (81%)
Frame = +3
Query: 153 QTYHREVREGGPGNG*RILQICLGIGQTKXXXXXXITIDIALWKFETSKYYVTIIDAPGH 332
+ + +E +E G G+ + + + K ITIDIALWKFETSKYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 95
Query: 333 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 509
RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 147 bits (357), Expect = 9e-38
Identities = 68/69 (98%), Positives = 68/69 (98%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 229 DKLKAERER 255
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 109 bits (262), Expect = 3e-26
Identities = 50/54 (92%), Positives = 51/54 (94%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSPK 667
+MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S K
Sbjct: 154 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 207
Score = 28.7 bits (61), Expect = 0.066
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 661 TKMPWXKGWQVGAVREGKA*PENAXLKPLDAXL 759
+KMPW KGW V +EGK ++ LDA L
Sbjct: 206 SKMPWFKGWTVER-KEGKV-EGKCLIEALDAIL 236
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 175 bits (427), Expect = 3e-46
Identities = 84/84 (100%), Positives = 84/84 (100%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN
Sbjct: 14 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 73
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
GQTREHALLAFTLGVKQLIVGVNK
Sbjct: 74 GQTREHALLAFTLGVKQLIVGVNK 97
Score = 109 bits (262), Expect = 3e-26
Identities = 50/54 (92%), Positives = 51/54 (94%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSPK 667
+MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S K
Sbjct: 97 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 150
Score = 29.5 bits (63), Expect = 0.038
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +1
Query: 220 WVLDKLKAERER 255
WVLDKLKAERER
Sbjct: 1 WVLDKLKAERER 12
Score = 28.7 bits (61), Expect = 0.066
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 661 TKMPWXKGWQVGAVREGKA*PENAXLKPLDAXL 759
+KMPW KGW V +EGK ++ LDA L
Sbjct: 149 SKMPWFKGWTVER-KEGKV-EGKCLIEALDAIL 179
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 173 bits (422), Expect = 1e-45
Identities = 87/119 (73%), Positives = 96/119 (80%)
Frame = +3
Query: 153 QTYHREVREGGPGNG*RILQICLGIGQTKXXXXXXITIDIALWKFETSKYYVTIIDAPGH 332
+ + +E +E G G+ + + + K ITIDIALWKFET+KYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGH 95
Query: 333 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 509
RDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 147 bits (357), Expect = 9e-38
Identities = 68/69 (98%), Positives = 68/69 (98%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 229 DKLKAERER 255
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 108 bits (260), Expect = 5e-26
Identities = 48/54 (88%), Positives = 51/54 (94%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSPK 667
+MD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPSPK
Sbjct: 154 KMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPK 207
Score = 25.0 bits (52), Expect = 0.81
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 664 KMPWXKGWQVGAVREGKA*PENAXLKPLDAXL 759
K PW KGW+V ++G A ++ LDA L
Sbjct: 207 KTPWYKGWKVER-KDGNA-DGKTLIEALDAIL 236
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 170 bits (414), Expect = 1e-44
Identities = 81/81 (100%), Positives = 81/81 (100%)
Frame = +3
Query: 267 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 446
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 447 REHALLAFTLGVKQLIVGVNK 509
REHALLAFTLGVKQLIVGVNK
Sbjct: 61 REHALLAFTLGVKQLIVGVNK 81
Score = 109 bits (262), Expect = 3e-26
Identities = 50/54 (92%), Positives = 51/54 (94%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSPK 667
+MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S K
Sbjct: 81 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 134
Score = 28.7 bits (61), Expect = 0.066
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 661 TKMPWXKGWQVGAVREGKA*PENAXLKPLDAXL 759
+KMPW KGW V +EGK ++ LDA L
Sbjct: 133 SKMPWFKGWTVER-KEGKV-EGKCLIEALDAIL 163
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 306 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.62
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 61 KTHINIVVIGHVDSGKST 114
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.4
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 70 INIVVIGHVDSGKST 114
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.3
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 62 FSLPIFG*SRITNCV*Y 12
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.3
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 392
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.3
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 392
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex
determiner protein.
Length = 182
Score = 22.2 bits (45), Expect = 5.7
Identities = 10/44 (22%), Positives = 19/44 (43%)
Frame = +2
Query: 494 RRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 625
R K + S Y+ + ++Y KK+ YN + +P+
Sbjct: 77 REPKIISSLSNNYNYSNYNNYNNNYNNYNKKLYYNINYIEQIPV 120
>AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein 1
protein.
Length = 500
Score = 22.2 bits (45), Expect = 5.7
Identities = 11/35 (31%), Positives = 15/35 (42%)
Frame = -3
Query: 711 AFPYGSNLPSLXPRHFGEGSNMLSPCHPEMGTKAT 607
+F Y L H+GE + CH G+K T
Sbjct: 240 SFGYNHVLKLHQVAHYGEKVYKCTLCHETFGSKKT 274
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 225,180
Number of Sequences: 438
Number of extensions: 5016
Number of successful extensions: 32
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 25246416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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