BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0748.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ... 161 7e-41 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 29 0.64 SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomy... 28 1.5 SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p... 26 6.0 >SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 161 bits (392), Expect = 7e-41 Identities = 80/144 (55%), Positives = 102/144 (70%), Gaps = 2/144 (1%) Frame = -3 Query: 657 KXXKLXSIXLFSLPIKEFE--DH*FLPRPVPE**GSKDHACTETNTCRTAHTFKAFVAIG 484 K + I L+SLPIKE++ D+ FLPR E K + FKAFV IG Sbjct: 48 KIKSIEEIYLYSLPIKEYQIVDY-FLPRLNDE--VMKVVPVQKQTRAGQRTRFKAFVVIG 104 Query: 483 DNNGHIGLGVKCSKEVATAIRGAIILAKLSLLPVRRGYWGNKIGKPHTVPCKVTGKCGSV 304 D++GH+GLG+KC+KEVATAIRGAII+ KLS++P+RRGYWG +G PHTVP KV+GKCGSV Sbjct: 105 DSDGHVGLGIKCAKEVATAIRGAIIMGKLSIMPIRRGYWGTALGDPHTVPVKVSGKCGSV 164 Query: 303 TVRLIPAPRGTGIVSAQFLRSFFR 232 TVRL+PAPRG G+V+A + F + Sbjct: 165 TVRLVPAPRGAGLVAAPVTKRFLQ 188 Score = 62.9 bits (146), Expect = 4e-11 Identities = 27/61 (44%), Positives = 37/61 (60%) Frame = -2 Query: 253 VPKKLLQMAGVQDCYTSARGSTGTLGNFXXXXXXXXXXXXXYLTPDLWRDIPLTKSPYSE 74 V K+ LQ+AG++DCYT +RGST TLGNF LTP+LW + P ++P E Sbjct: 182 VTKRFLQLAGIEDCYTQSRGSTKTLGNFVKAAFAAASLTYGILTPNLWAERPFGQTPIEE 241 Query: 73 F 71 + Sbjct: 242 Y 242 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 29.1 bits (62), Expect = 0.64 Identities = 12/20 (60%), Positives = 16/20 (80%) Frame = -3 Query: 246 RSFFRWLVYRTATPQLVVQL 187 RS F+WL+ TATP+L+V L Sbjct: 69 RSVFQWLIALTATPRLLVLL 88 >SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 465 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/41 (29%), Positives = 25/41 (60%) Frame = -1 Query: 626 FLYQSKNSKIIDFFLGPSLNDKVLKIMPVQKQTRAGQRTLS 504 +L + +++D GP + DK+LKI+P +++T T++ Sbjct: 192 YLIMDEADRLLDMDFGPII-DKILKIIPHERRTLLFSATMT 231 >SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/29 (34%), Positives = 15/29 (51%) Frame = -2 Query: 352 KATHRPLQGHRQVWFCNSPADSCPSWYWN 266 +A L G + C + SCPS++WN Sbjct: 153 RADRAKLDGLYECILCACCSTSCPSYWWN 181 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,845,155 Number of Sequences: 5004 Number of extensions: 59827 Number of successful extensions: 166 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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