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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0745.Seq
         (628 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein L13/L16|Schiz...   137   1e-33
SPBC839.13c |rpl1601||60S ribosomal protein L13/L16|Schizosaccha...   137   1e-33
SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizos...   135   4e-33
SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyce...    29   0.73 
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po...    27   2.2  
SPCC777.07 |||alpha-1,2-mannosyltransferase |Schizosaccharomyces...    26   3.9  
SPBC16G5.04 |mrpl23||mitochondrial ribosomal protein subunit L13...    26   5.1  
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa...    25   6.8  
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    25   9.0  

>SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 197

 Score =  137 bits (331), Expect = 1e-33
 Identities = 61/88 (69%), Positives = 74/88 (84%)
 Frame = +3

Query: 3   RGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNVNPARGPF 182
           +GHLLGRLA+V+AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG F
Sbjct: 13  KGHLLGRLASVVAKQLLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAF 72

Query: 183 HFRAPSKILWKTVRGMIPHKTERGRMLL 266
           HFRAPS+I  K VRGM+PHKT RG+  L
Sbjct: 73  HFRAPSRIFQKAVRGMLPHKTARGQAAL 100



 Score = 79.8 bits (188), Expect = 3e-16
 Identities = 40/91 (43%), Positives = 50/91 (54%)
 Frame = +2

Query: 233 PTQNRAWQNALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIGWK 412
           P +    Q AL  L+  +G PPPFD               LKPGR YC VGRLS E+GWK
Sbjct: 90  PHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRLSSEVGWK 149

Query: 413 YRDVVRKLEDKRKGKAVKRVAYEKKLKRITK 505
           Y D+V KLE++RK K+     Y+ KL +  K
Sbjct: 150 YSDIVSKLEERRKVKSA--AFYQAKLAKQKK 178


>SPBC839.13c |rpl1601||60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 197

 Score =  137 bits (331), Expect = 1e-33
 Identities = 61/88 (69%), Positives = 74/88 (84%)
 Frame = +3

Query: 3   RGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNVNPARGPF 182
           +GHLLGRLA+V+AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG F
Sbjct: 13  KGHLLGRLASVVAKQLLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAF 72

Query: 183 HFRAPSKILWKTVRGMIPHKTERGRMLL 266
           HFRAPS+I  K VRGM+PHKT RG+  L
Sbjct: 73  HFRAPSRIFQKAVRGMLPHKTARGQAAL 100



 Score = 81.8 bits (193), Expect = 7e-17
 Identities = 44/113 (38%), Positives = 57/113 (50%)
 Frame = +2

Query: 233 PTQNRAWQNALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIGWK 412
           P +    Q AL  L+  +G PPPFD               LKPGR YC VGRLS E+GWK
Sbjct: 90  PHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRLSSEVGWK 149

Query: 413 YRDVVRKLEDKRKGKAVKRVAYEKKLKRITKDLVRRCPKATTPFTTIIQSYGY 571
           Y D+V KLE++RK K+     Y+ KL +  K       K  +P    +  +GY
Sbjct: 150 YNDIVAKLEERRKVKSA--AFYQAKLAKQKK---IASAKEASPVNQKLSQFGY 197


>SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 197

 Score =  135 bits (327), Expect = 4e-33
 Identities = 59/88 (67%), Positives = 73/88 (82%)
 Frame = +3

Query: 3   RGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNVNPARGPF 182
           +GHL+GRLA+ +AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG F
Sbjct: 13  KGHLMGRLASTVAKQLLAGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAF 72

Query: 183 HFRAPSKILWKTVRGMIPHKTERGRMLL 266
           HFRAPS+I  K VRGM+PHKT RG + L
Sbjct: 73  HFRAPSRIFTKAVRGMLPHKTTRGNIAL 100



 Score = 72.9 bits (171), Expect = 3e-14
 Identities = 31/67 (46%), Positives = 42/67 (62%)
 Frame = +2

Query: 260 ALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIGWKYRDVVRKLE 439
           AL+ L+  +G PPPFD               LKP R YC +GRLS E+GWKY+++V KLE
Sbjct: 99  ALKNLQALEGIPPPFDKQKRLVVPAALRVLRLKPSRKYCTIGRLSSEVGWKYKNIVSKLE 158

Query: 440 DKRKGKA 460
           ++RK K+
Sbjct: 159 ERRKIKS 165


>SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 506

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = +3

Query: 9   HLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFF 113
           H L  + AV  K++  G K VV+RC+ I  + +FF
Sbjct: 157 HHLEDVKAVGKKLIKAGCKNVVIRCDDIPFASDFF 191


>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 474

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 16/41 (39%), Positives = 21/41 (51%)
 Frame = +2

Query: 386 RLSHEIGWKYRDVVRKLEDKRKGKAVKRVAYEKKLKRITKD 508
           R S E   K  D   K E+KR+ K  K+   EKK K+  K+
Sbjct: 428 RKSVESSEKDEDEAAKKEEKRRKKEAKKEKKEKKEKKEKKE 468


>SPCC777.07 |||alpha-1,2-mannosyltransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 378

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -2

Query: 258 FCHALFCVGSYLLQSSIES*MEL 190
           FC  LFC+ S+ LQSS +   EL
Sbjct: 10  FCILLFCIISFYLQSSKDGPKEL 32


>SPBC16G5.04 |mrpl23||mitochondrial ribosomal protein subunit
           L13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 157

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 8/22 (36%), Positives = 13/22 (59%)
 Frame = +2

Query: 239 QNRAWQNALRRLRTYDGCPPPF 304
           +N+ W   ++RL  YDG   P+
Sbjct: 113 RNKLWDRRMKRLYIYDGAEHPY 134


>SPAC1B9.02c |sck1||serine/threonine protein kinase
           Sck1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 696

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 15/55 (27%), Positives = 27/55 (49%)
 Frame = -1

Query: 193 ALKWKGPRAGFTLHLLRRNDISLSLFLKKLPEILICSQRTTTTLFPSRRTLAMTA 29
           AL+  G      ++L+R+ND +    +KK+ + LI  ++  T     R  L  T+
Sbjct: 304 ALRLIGKGTFGQVYLVRKNDTNRIYAMKKISKKLIVRKKEVTHTLGERNILVRTS 358


>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +3

Query: 117  NKLKLMSFLRKRCNVNPARGPFHFRAPSKILWKTVRGMI 233
            N+ + MS   KRC     RG  HF    + L+  ++ ++
Sbjct: 1323 NRTRQMSVYGKRCCNKSCRGSVHFEYNDEQLYNQIKFLL 1361


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,694,981
Number of Sequences: 5004
Number of extensions: 55799
Number of successful extensions: 152
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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