BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0743.Seq
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin fa... 31 0.61
At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family... 31 0.81
At1g26150.1 68414.m03192 protein kinase family protein similar t... 30 1.4
At1g13050.1 68414.m01513 expressed protein 30 1.9
At5g19910.1 68418.m02369 SOH1 family protein contains Pfam profi... 29 2.5
At3g11000.1 68416.m01328 expressed protein 29 2.5
At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid t... 29 2.5
At5g65410.1 68418.m08226 zinc finger homeobox family protein / Z... 29 3.3
At4g00890.1 68417.m00120 proline-rich family protein contains pr... 29 3.3
At5g07760.1 68418.m00888 formin homology 2 domain-containing pro... 29 4.3
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 29 4.3
At1g75000.1 68414.m08707 GNS1/SUR4 membrane family protein conta... 29 4.3
At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 29 4.3
At5g48360.1 68418.m05975 formin homology 2 domain-containing pro... 28 5.7
At1g21580.1 68414.m02698 hydroxyproline-rich glycoprotein family... 28 5.7
At3g19430.1 68416.m02464 late embryogenesis abundant protein-rel... 28 7.6
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 28 7.6
At5g61090.1 68418.m07665 proline-rich family protein contains pr... 27 10.0
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 27 10.0
At5g08520.1 68418.m01011 myb family transcription factor contain... 27 10.0
>At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245, SP|Q03211 Pistil-specific
extensin-like protein precursor (PELP) {Nicotiana
tabacum}; contains Pfam profile PF01190: Pollen proteins
Ole e I family
Length = 239
Score = 31.5 bits (68), Expect = 0.61
Identities = 23/64 (35%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = -2
Query: 504 GNQDPVTKALPYSHPQPIRRAPTVP--AAPIPPQARPGQAQPLQAVLLPRAVQVHR--TL 337
G+ DP +LP P P + PT+P API A P P++ LP A + TL
Sbjct: 31 GSSDPF-HSLPQHLPLPPIKLPTLPPAKAPIKLPAYPPAKAPIKLPTLPPAKAPIKLPTL 89
Query: 336 PPLR 325
PP++
Sbjct: 90 PPIK 93
>At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family
protein identical to proline-rich protein 2 [Arabidopsis
thaliana] gi|7620011|gb|AAF64549
Length = 321
Score = 31.1 bits (67), Expect = 0.81
Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
Frame = -2
Query: 471 YSHPQPIRRAPTV-PAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPL 328
Y P PI + P V P P PP+ +P + P V +T PPL
Sbjct: 216 YKPPVPIYKPPVVIPKKPCPPKIHKPIYKPPVPIYKPPVVIPKKTFPPL 264
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -2
Query: 501 NQDPVTKALPYSHPQPIRRAPTVPAAPIPPQARPGQAQPL-QAVLLPRAVQVHRTLPP 331
N P K +P SH P R P+ PA+ IPP R + P + P + H + PP
Sbjct: 165 NPLPPPKLVPPSHSPP-RHLPSPPASEIPPPPRHLPSPPASERPSTPPSDSEHPSPPP 221
>At1g13050.1 68414.m01513 expressed protein
Length = 317
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -2
Query: 474 PYSHPQPIRRAPTVPAAPIPPQARPGQAQPLQ 379
P S P P+R P P+PP+ P A+PLQ
Sbjct: 68 PSSRPLPLR-----PEEPLPPRHNPNSARPLQ 94
>At5g19910.1 68418.m02369 SOH1 family protein contains Pfam profile:
PF05669 SOH1
Length = 196
Score = 29.5 bits (63), Expect = 2.5
Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = -2
Query: 522 FWWWKEGNQDPVTKALPYSHPQPIRRAPTV-PAAPIPPQARPGQAQPLQAVLLP 364
F++WK + + LP P+P+ P V P+ +PP P L L P
Sbjct: 116 FYYWKNYRNNRLKHILPRPLPEPVPPQPPVAPSTSLPP--APSATAALSPALSP 167
>At3g11000.1 68416.m01328 expressed protein
Length = 488
Score = 29.5 bits (63), Expect = 2.5
Identities = 15/52 (28%), Positives = 22/52 (42%)
Frame = -2
Query: 528 DGFWWWKEGNQDPVTKALPYSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAV 373
D +W E ++ K L P P R PT+ +PP +P A L +
Sbjct: 120 DDKMFWFELDRGQTNKLLRLFKPSPSVRPPTISRDAVPPPRKPIPASSLAQI 171
>At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to auxin
down regulated GB:X69640 GI:296442 from [Glycine max];
contains Pfam profile PF00234: Protease inhibitor/seed
storage/LTP family
Length = 297
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/67 (32%), Positives = 27/67 (40%), Gaps = 4/67 (5%)
Frame = -2
Query: 492 PVTKALPYSHPQPIRRAPTV--PAAPIPPQARPGQAQPLQAV--LLPRAVQVHRTLPPLR 325
PV P P PI R P + P P P PG P+ LLP LPP+
Sbjct: 109 PVVYPPPIVRPPPITRPPIIIPPIQPPPVTTPPGLLPPITTPPGLLPPVTTPPGLLPPVT 168
Query: 324 LVFPGVI 304
PG++
Sbjct: 169 TP-PGLL 174
>At5g65410.1 68418.m08226 zinc finger homeobox family protein /
ZF-HD homeobox family protein similar to hypothetical
proteins (GP|4220524)(GP|3184285|)(Arabidopsis); ZP-HD
homeobox family protein GP|13374061 (Flaveria
bidentis);GP:5091602 {Oryza sativa}
Length = 279
Score = 29.1 bits (62), Expect = 3.3
Identities = 18/47 (38%), Positives = 21/47 (44%), Gaps = 7/47 (14%)
Frame = -2
Query: 483 KALPYSHPQPIRRAPTVPA-------APIPPQARPGQAQPLQAVLLP 364
K LPY H P + P P AP+ + P QA PLQ L P
Sbjct: 123 KELPYFHHAPPQHQPPPPPPGFYRLPAPVSYRPPPSQAPPLQLALPP 169
>At4g00890.1 68417.m00120 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 431
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = -2
Query: 474 PYSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTL 337
P HPQ ++P +P+ P+++ L+ + V+ HR L
Sbjct: 47 PLFHPQSPPEPKSLPLSPLSPKSKEEPESQTMTPLMSKHVKTHRNL 92
>At5g07760.1 68418.m00888 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 853
Score = 28.7 bits (61), Expect = 4.3
Identities = 18/52 (34%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
Frame = -2
Query: 462 PQPIRRAPTVPAAPIPPQARPG-QAQPLQAVLLPRAVQVHRTLPPLRLVFPG 310
P P R +P P PP P A+ L P V+ LPP L+F G
Sbjct: 58 PPPAMRRRVLPRPPPPPPPLPMFDAEVLCCCYPPTRVRREAPLPPPPLIFVG 109
>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 340
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/56 (26%), Positives = 26/56 (46%)
Frame = -2
Query: 501 NQDPVTKALPYSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLP 334
+Q P T L P P + ++P+ P PP + A+P+ + + + RT P
Sbjct: 185 HQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSSSFHAEPIPPTVGTSSTKRRRTTP 240
>At1g75000.1 68414.m08707 GNS1/SUR4 membrane family protein contains
Pfam profile PF01151: GNS1/SUR4 family
Length = 281
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = -3
Query: 380 RQFFYLGRFKYIGRFLHFV*CFQESSFRREQTFTQAWSRKNMLC 249
R FF+ F Y+ RFLH F RR+ +F Q ++ ++LC
Sbjct: 125 RVFFWSYAF-YLSRFLHLFRTFFSVIRRRKLSFFQLINQSSLLC 167
>At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245; contains Pfam profile
PF01190: Pollen proteins Ole e I family
Length = 359
Score = 28.7 bits (61), Expect = 4.3
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = -2
Query: 474 PYSHPQPIRRAPTVPA--APIPPQARPGQAQPL 382
P+ HP P ++P P AP+ P A+P P+
Sbjct: 60 PHPHPHPPAKSPVKPPVKAPVSPPAKPPVKPPV 92
>At5g48360.1 68418.m05975 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 782
Score = 28.3 bits (60), Expect = 5.7
Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 7/58 (12%)
Frame = -2
Query: 474 PYSHPQ-PIRRAPTVPAAPIPPQAR------PGQAQPLQAVLLPRAVQVHRTLPPLRL 322
P S P P+ +P P P+PP P + A++LPR+ + H T P L L
Sbjct: 47 PISPPFFPLESSPPSPPPPLPPTPPTTFAVFPTFPANISALVLPRSSKPHHTSPTLLL 104
>At1g21580.1 68414.m02698 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 1696
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/41 (36%), Positives = 19/41 (46%)
Frame = -2
Query: 474 PYSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQ 352
P+ HP AP P P+PP P Q+ P L R+ Q
Sbjct: 19 PHLHPPS---APLPPPPPLPPPPPPRQSHPESPNLYGRSTQ 56
>At3g19430.1 68416.m02464 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 559
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/50 (32%), Positives = 19/50 (38%), Gaps = 2/50 (4%)
Frame = -2
Query: 507 EGNQDPVTKALPYSHPQPIRR--APTVPAAPIPPQARPGQAQPLQAVLLP 364
+G P P S P P +PT P +P PP P P V P
Sbjct: 69 DGGYTPPAPVPPVSPPPPTPSVPSPTPPVSPPPPTPTPSVPSPTPPVSPP 118
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 471 YSHPQPIRRAPTVPAAPIPPQARP 400
YS P P+ P P P+PP P
Sbjct: 809 YSPPPPVFSPPPKPVTPLPPATSP 832
>At5g61090.1 68418.m07665 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
contains similarity to vegetative cell wall protein gp1
[Chlamydomonas reinhardtii] gi|12018147|gb|AAG45420;
common family members: At4g18570, At3g25690, At4g04980
[Arabidopsis thaliana]
Length = 344
Score = 27.5 bits (58), Expect = 10.0
Identities = 22/59 (37%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
Frame = -2
Query: 486 TKALPYSHPQ-PIRRAPT-VPAAPIPPQARPGQAQPLQAVLLP---RAVQVHRTLPPLR 325
T P S P PI P VPA P P RP A+P + P R + +TL P R
Sbjct: 100 TPEAPRSVPACPIPEIPRPVPARPTPETPRPVTARPTPEIPRPVPARPISEVQTLVPTR 158
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = -2
Query: 465 HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQV--HRTLPP 331
HPQ + P +P +PP + PL LPR +Q+ H +PP
Sbjct: 580 HPQQMLPMPNMPHHQLPPSSH----MPLHPHHLPRPMQMPPHGHMPP 622
>At5g08520.1 68418.m01011 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 298
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/46 (34%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
Frame = -2
Query: 474 PYSHPQPIRRAPTVPAAPIPPQARPGQAQPL--QAVLLPRAVQVHR 343
P + P P A V AAP+P PG A + +PR HR
Sbjct: 253 PVNLPAPPHMAYGVHAAPVPGSVVPGAAMNIGQMPYTMPRTPTAHR 298
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,098,907
Number of Sequences: 28952
Number of extensions: 307837
Number of successful extensions: 1152
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1011
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1144
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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