BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0740.Seq
(527 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60670.1 68418.m07614 60S ribosomal protein L12 (RPL12C) 60S ... 57 6e-09
At3g53430.1 68416.m05896 60S ribosomal protein L12 (RPL12B) 60S ... 53 1e-07
At2g37190.1 68415.m04562 60S ribosomal protein L12 (RPL12A) 52 2e-07
At2g28720.1 68415.m03491 histone H2B, putative strong similarity... 29 1.9
At5g50830.1 68418.m06297 expressed protein 28 3.4
At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai... 27 5.9
At4g31260.1 68417.m04437 hypothetical protein 27 5.9
At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 ... 27 5.9
At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family... 27 5.9
>At5g60670.1 68418.m07614 60S ribosomal protein L12 (RPL12C) 60S
RIBOSOMAL PROTEIN L12 (like), Arabidopsis thaliana,
PIR:T45883
Length = 166
Score = 57.2 bits (132), Expect = 6e-09
Identities = 26/42 (61%), Positives = 34/42 (80%)
Frame = -1
Query: 509 MARYLSGSVKEILGTAQSVGCTVEGRPPHDLIDDINSGALXI 384
+A+ LSG+VKEILGT SVGCTV+G+ P DL ++INSG + I
Sbjct: 122 IAKELSGTVKEILGTCVSVGCTVDGKDPKDLQEEINSGDIDI 163
>At3g53430.1 68416.m05896 60S ribosomal protein L12 (RPL12B) 60S
RIBOSOMAL PROTEIN L12, Prunus armeniaca,
SWISSPROT:RL12_PRUAR
Length = 166
Score = 53.2 bits (122), Expect = 1e-07
Identities = 24/44 (54%), Positives = 32/44 (72%)
Frame = -1
Query: 509 MARYLSGSVKEILGTAQSVGCTVEGRPPHDLIDDINSGALXIXE 378
+A+ LSG+V+EILGT SVGCTV+G+ P DL +I G + I E
Sbjct: 122 IAKELSGTVREILGTCVSVGCTVDGKDPKDLQQEIQEGEIEIPE 165
>At2g37190.1 68415.m04562 60S ribosomal protein L12 (RPL12A)
Length = 166
Score = 52.0 bits (119), Expect = 2e-07
Identities = 23/44 (52%), Positives = 32/44 (72%)
Frame = -1
Query: 509 MARYLSGSVKEILGTAQSVGCTVEGRPPHDLIDDINSGALXIXE 378
+A+ LSG+V+EILGT SVGCTV+G+ P D+ +I G + I E
Sbjct: 122 IAKELSGTVREILGTCVSVGCTVDGKDPKDIQQEIQDGEVEIPE 165
>At2g28720.1 68415.m03491 histone H2B, putative strong similarity to
histone H2B Arabidopsis thaliana GI:2407802, Gossypium
hirsutum SP|O22582; contains Pfam profile PF00125 Core
histone H2A/H2B/H3/H4
Length = 151
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = +3
Query: 138 APKKGRGKAKVVEDPETESVEAEVDEKNIVSEKKTKRGRKLP 263
APK G+ A+ + + E +V EK +EKK K G+KLP
Sbjct: 2 APKAGKKPAEKKPAEKAPAEEEKVAEK-APAEKKPKAGKKLP 42
>At5g50830.1 68418.m06297 expressed protein
Length = 281
Score = 28.3 bits (60), Expect = 3.4
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 6/50 (12%)
Frame = +3
Query: 135 DAPKKGRGKAKVVEDP----ETESVEAEVDEKNIV--SEKKTKRGRKLPV 266
D P + K VED ETESV E+ E + EKK +RG++ +
Sbjct: 197 DVPSDDDDEEKDVEDARKSMETESVTTEIKEDGSIVKKEKKERRGKRFGI 246
>At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains
Pfam profiles PF00270:DEAD/DEAH box helicase and
PF00271: Helicase conserved C-terminal domain;
identical to cDNA RH27 helicase, partial GI:4033334
Length = 633
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 233 KENQTRKEAAGDTNGQDENGKIEETAPKKKSGRE 334
K+ + + GDTN +++ EE PKKK ++
Sbjct: 90 KKKNKKLQQRGDTNDEEDEVIAEEEEPKKKKKKQ 123
>At4g31260.1 68417.m04437 hypothetical protein
Length = 63
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1
Query: 364 IRNIYSXMXKAPLLMSSIRSCGGLPSTV 447
IR++Y K+ + + IRSCGG+ ++V
Sbjct: 28 IRSLYPDKVKSEIPIQDIRSCGGVLASV 55
>At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 (4
significant) WD-40 repeats (PF0400); identical to WD-40
repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 507
Score = 27.5 bits (58), Expect = 5.9
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +3
Query: 141 PKKGRGKAKVVEDPETESVEAEVDEKNIVSEKKTKR 248
PKK K K ED +T S + + D K S KKT++
Sbjct: 25 PKKRGRKPKTKEDSQTPSSQQQSDVKMKESGKKTQQ 60
>At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family
protein
Length = 635
Score = 27.5 bits (58), Expect = 5.9
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -3
Query: 336 NSRPLFFFGAVSSILPFSSCPFVSPAASFRVW 241
+S P F A ++I SS P SPA+S R+W
Sbjct: 142 SSSPFSFGSAPAAITSVSSGPAQSPASSPRLW 173
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,476,519
Number of Sequences: 28952
Number of extensions: 133281
Number of successful extensions: 522
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 521
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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