Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0735.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53800.1 68418.m06685 expressed protein 32 0.33
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 31 0.44
At1g56660.1 68414.m06516 expressed protein 31 0.44
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 30 1.0
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 30 1.0
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 29 1.8
At1g31460.1 68414.m03852 expressed protein 29 1.8
At5g13090.1 68418.m01500 expressed protein predicted proteins - ... 29 2.4
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 29 2.4
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 29 2.4
At3g29075.1 68416.m03637 glycine-rich protein 29 3.1
At2g22795.1 68415.m02704 expressed protein 29 3.1
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 29 3.1
At1g10890.1 68414.m01251 F-box family protein contains Pfam PF00... 29 3.1
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 28 4.1
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 28 4.1
At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing ... 28 5.4
At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase, p... 28 5.4
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 5.4
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 28 5.4
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 28 5.4
At3g28110.1 68416.m03508 hypothetical protein 27 7.2
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 9.5
At4g27310.1 68417.m03918 zinc finger (B-box type) family protein... 27 9.5
At3g02810.1 68416.m00273 protein kinase family protein contains ... 27 9.5
At2g16140.1 68415.m01850 expressed protein contains similarity t... 27 9.5
At1g65190.1 68414.m07391 protein kinase family protein contains ... 27 9.5
>At5g53800.1 68418.m06685 expressed protein
Length = 351
Score = 31.9 bits (69), Expect = 0.33
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 105
+R +RE +EK+ K R+ K KR +D ++ KE+ K +K
Sbjct: 136 KRKEREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSEK 180
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 31.5 bits (68), Expect = 0.44
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 7/52 (13%)
Frame = -3
Query: 245 PSERNQREVKEK--KSKANMR--KTTKGKRTTADPPNI---QREKERVWKNQ 111
P ER +++ KEK KSK + R K K K + P+ Q++K R WKN+
Sbjct: 611 PKERKEKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNE 662
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 31.5 bits (68), Expect = 0.44
Identities = 28/126 (22%), Positives = 48/126 (38%)
Frame = -3
Query: 506 DLKDELTEDXXXXXXXXKDEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXX 327
D+K E E K E L EKEG K + + E E + D +
Sbjct: 105 DVKVEEHEKEHKKGKEKKHEELEEEKEGKKKKNKKEKDESGPEEKNKKADKEKKHEDVSQ 164
Query: 326 XXXXXXXXXXXERRTHDKFSIGKNVLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPN 147
+ + +K G + +++ KE+ SK+N K KGK+ + +
Sbjct: 165 EKEELEEEDGKKNKKKEKDESGTEEKKKKPKKEKKQKEE-SKSNEDKKVKGKKEKGEKGD 223
Query: 146 IQREKE 129
+++E E
Sbjct: 224 LEKEDE 229
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 30.3 bits (65), Expect = 1.0
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 108
E+ +E KEKK K K K K ++EKER K +K
Sbjct: 79 EKKDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERK 122
Score = 29.1 bits (62), Expect = 2.4
Identities = 17/60 (28%), Positives = 28/60 (46%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 60
E +++ KE+K K K K K+ ++EKER K +K S + R ++P
Sbjct: 84 EEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAAARYRILSP 143
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 30.3 bits (65), Expect = 1.0
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -3
Query: 227 REVKEKKSKANMRKTTKGKRTTADPPNIQREK-ERVWKNQKKGTASSRLSPSR 72
REV +K+ ++ T K +RT D R + ER + SSR SP R
Sbjct: 65 REVGDKRRRSGREDTEKRRRTRTDDERYSRGRHERSTSPSDRSHRSSRRSPER 117
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKG 102
++ +RE KEKKSK + K ++ D Q++KE K ++KG
Sbjct: 23 DKEKREGKEKKSKDRSKDKQKERKEKKDKHKDQKDKE---KGKEKG 65
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/69 (23%), Positives = 33/69 (47%)
Frame = -3
Query: 254 VLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPS 75
+L P ++ ++ K+ + + ++ A PP+ E E+ K + K + +LSPS
Sbjct: 149 ILSPVKQQTKKKLPKEKRRIVSPSSSSSIDLATPPSTDSEPEKKSKPKSKSSWFDKLSPS 208
Query: 74 RWVNPA*RS 48
+ + RS
Sbjct: 209 KLIGSIWRS 217
>At5g13090.1 68418.m01500 expressed protein predicted proteins -
Arabidopsis thaliana
Length = 269
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = -2
Query: 267 HWQERISAERTKSKRGERKEVEGEYEKD 184
H E SA R+KSKRG++KE G D
Sbjct: 134 HHGEENSASRSKSKRGKKKEKPGRRVTD 161
>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 2.4
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 245 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 129
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 2.4
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 245 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 129
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At3g29075.1 68416.m03637 glycine-rich protein
Length = 294
Score = 28.7 bits (61), Expect = 3.1
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -3
Query: 434 EKEGYKSDYDRNEYEERGSEHQEDNDSD 351
+KE YK +D ++Y+E+ + ++ ND D
Sbjct: 189 KKEQYKEHHDDDDYDEKKKKKKDYNDDD 216
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 28.7 bits (61), Expect = 3.1
Identities = 27/146 (18%), Positives = 60/146 (41%), Gaps = 3/146 (2%)
Frame = -3
Query: 533 QKETSGCRQDLKDELTEDXXXXXXXXKDEGLTLEKEGYKSDYDRNEYEERGSEHQ-EDND 357
+KE S ++ +++ TE ++E T +KE K + + +E E++ E +
Sbjct: 510 EKEESSSQEKTEEKETETKDNEESSSQEE--TKDKENEKIEKEEASSQEESKENETETKE 567
Query: 356 SDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKNVLVPSERN--QREVKEKKSKANMRKT 183
+ E ++ +N + E + Q E KEK+++ ++
Sbjct: 568 KEESSSQEETKEKENEKIEKEESAPQEETKEKENEKIEKEESASQEETKEKETETKEKEE 627
Query: 182 TKGKRTTADPPNIQREKERVWKNQKK 105
+ + + +KE+V +N+KK
Sbjct: 628 SSSNESQENVNTESEKKEQVEENEKK 653
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 28.7 bits (61), Expect = 3.1
Identities = 27/110 (24%), Positives = 49/110 (44%)
Frame = -3
Query: 434 EKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKN 255
E+E + D DR +R SE ++ +DS+ R H++ GK+
Sbjct: 78 ERERRRRDKDR---VKRRSERRKSSDSEDDVEEEDERDKRRVNEKERGHREHER-DRGKD 133
Query: 254 VLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 105
+ RE +E+K K R+ + +R + +REKERV + +++
Sbjct: 134 -----RKRDREREERKDKEREREKDRERR---EREREEREKERVKERERR 175
>At1g10890.1 68414.m01251 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 592
Score = 28.7 bits (61), Expect = 3.1
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -3
Query: 242 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRL 84
+E N + V+E + K M + K + + +QR+KE + +K RL
Sbjct: 159 AEENLKRVEEAQRKEAMERQRKEEERYRELEELQRQKEEAMRRKKAEEEEERL 211
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.3 bits (60), Expect = 4.1
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 242 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 111
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 455 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 497
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 28.3 bits (60), Expect = 4.1
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 242 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 111
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 408 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 450
>At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing
protein
Length = 527
Score = 27.9 bits (59), Expect = 5.4
Identities = 26/101 (25%), Positives = 40/101 (39%), Gaps = 2/101 (1%)
Frame = -3
Query: 434 EKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKN 255
E+E ++S D++ ++ E D +SD ERR +K S
Sbjct: 76 ERERHRSSRDKDRERDKVREGSRDKESDRERSSKERDRSDRDKPRDRERREREKRS---- 131
Query: 254 VLVPSERNQREVKEKK--SKANMRKTTKGKRTTADPPNIQR 138
S R++RE KE + + + R K ADP QR
Sbjct: 132 ----SSRSRREEKEPEVVERGSRRHRDKKDEPEADPERDQR 168
>At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 362
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +2
Query: 8 FDSSLSEYDFELSCFFKPDLPNEKDLDD 91
+ S + + LSCF PD+P +DL D
Sbjct: 142 YSSPSANWRDTLSCFMAPDVPETEDLPD 169
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 266 WRTCRGSYVLHTFPLHRIFRIRNLCCMSRRCR 361
W TC YVL+ +P+ I+ L C ++ CR
Sbjct: 646 WTTCPYCYVLYEYPI--IYEESVLKCQTKSCR 675
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 27.9 bits (59), Expect = 5.4
Identities = 20/53 (37%), Positives = 24/53 (45%)
Frame = -3
Query: 218 KEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 60
KEK+ KA KTTK +R +E +R K KG S R S NP
Sbjct: 284 KEKEPKALKDKTTKAERRAI------QEAQRAAKAAAKGEGSRRADESGRANP 330
>At1g44910.1 68414.m05146 FF domain-containing protein / WW
domain-containing protein contains Pfam profiles PF01846:
FF domain, PF00397: WW domain
Length = 946
Score = 27.9 bits (59), Expect = 5.4
Identities = 26/120 (21%), Positives = 50/120 (41%), Gaps = 2/120 (1%)
Frame = -3
Query: 434 EKEGYKSDYDRNEYEERGSEH--QEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIG 261
+K+ + + +R +E+G E +E++D + +R+ +
Sbjct: 820 DKDKERREKEREREKEKGKERSKREESDGETAMDVSEGHKDEKRKGKDRDRKHRRRHHNN 879
Query: 260 KNVLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLS 81
+ V S+R+ R+ +K S+ + K ++ P + E E K QKK SSR S
Sbjct: 880 SDEDVSSDRDDRDESKKSSRKHGNDRKKSRKHANSP---ESESENRHKRQKK--ESSRRS 934
>At3g28110.1 68416.m03508 hypothetical protein
Length = 354
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -3
Query: 449 EGLTLEKEGYKSDYDRNEYEERGSEHQEDND 357
E + +++E + D NEYE+ +E ED D
Sbjct: 132 EDINVDEEDDIDEGDENEYEDEDNEEDEDMD 162
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 105
E Q+E E K RK ++ K+ + Q+E K ++K
Sbjct: 747 EETQKEANESTKKERKRKKSESKKQSDGEEETQKEPSESTKKERK 791
>At4g27310.1 68417.m03918 zinc finger (B-box type) family protein
zinc-finger protein S3574, Oryza sativa, PIR3:JE0113
Length = 223
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = -3
Query: 452 DEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 351
D G + E Y D + +E EE + ED+D D
Sbjct: 110 DHGDGDDAESYDDDEEEDEDEEYSDDEDEDDDED 143
>At3g02810.1 68416.m00273 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 558
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = -3
Query: 488 TEDXXXXXXXXKDEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 351
TED ++ L EKE + + ++EE S + D++SD
Sbjct: 385 TEDDKSSTSSGEESSLESEKESVSKNEYKKKHEEEDSSMESDDESD 430
>At2g16140.1 68415.m01850 expressed protein contains similarity to
hypothetical proteins
Length = 311
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = -3
Query: 239 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 105
E R + K +KA +KT T D N E + +W+ ++K
Sbjct: 211 EAMSRPIGVKAAKAKAKKTVTKTTTVEDKGNAMLEIQSIWEIKQK 255
>At1g65190.1 68414.m07391 protein kinase family protein contains
similarity to protein kinases
Length = 396
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3
Query: 446 GLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 351
GLTL GYKS ++ EE+ SE ++ D+D
Sbjct: 238 GLTLLL-GYKSYFEHYRGEEKESEEEDPEDTD 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,454,223
Number of Sequences: 28952
Number of extensions: 135374
Number of successful extensions: 757
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 743
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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