BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0732.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ... 96 5e-21 SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual 30 0.37 SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 29 0.85 SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 28 1.1 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.5 SPAC7D4.09c |||steroid dehydrogenase |Schizosaccharomyces pombe|... 26 4.5 SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 26 4.5 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 26 4.5 SPBC31E1.02c |pmr1||P-type ATPase, calcium transporting Pmr1 |Sc... 26 6.0 SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 26 6.0 SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|ch... 25 7.9 SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 25 7.9 >SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 95.9 bits (228), Expect = 5e-21 Identities = 43/69 (62%), Positives = 52/69 (75%) Frame = -2 Query: 238 KSWRVQAVPIDPMSFVLRKALHSAWRGIRDDNLSSVSGIEGCIFCHSNGFIGGNTTLEGA 59 K+WRVQAV IDP SF R L WRGIRD+ LS ++GI GCIF H++GFIGGN T EGA Sbjct: 252 KAWRVQAVSIDPTSFTCRLPLPEPWRGIRDEKLSELTGIPGCIFVHASGFIGGNQTFEGA 311 Query: 58 LKMAIASLE 32 L+MA +L+ Sbjct: 312 LEMARKALD 320 Score = 93.9 bits (223), Expect = 2e-20 Identities = 53/140 (37%), Positives = 76/140 (54%), Gaps = 4/140 (2%) Frame = -3 Query: 666 EDLKLIYKKVYESFIQGIDAIDNGIPMTEE--QPKYDIHTHLSNRVKRLEP--RMELTQE 499 +DL+ +Y+KVY+SF++G+DA DNGI +P + L V P E + Sbjct: 110 QDLETLYEKVYQSFVEGLDANDNGISAYPAGLKPSFKAAMSLPEMVSSFLPAWNSEKQDD 169 Query: 498 TNVDEFFNKAMALVSEDFLYFTRYYINTWFPARNLVRKAIDNRFQIHKSGEIVVFNERFP 319 E F KA L+ F+ +Y +W PA+ L R+AI K I++ ++ FP Sbjct: 170 QTYLECFQKASDLMGTWFVRSVEHYALSWLPAKTLAREAILKA----KDSPILIVDQFFP 225 Query: 318 WKYHLFDLEQELGIEPQIKY 259 WK HLFD+E+ELGIE Q KY Sbjct: 226 WKGHLFDIEKELGIENQFKY 245 >SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual Length = 653 Score = 29.9 bits (64), Expect = 0.37 Identities = 24/133 (18%), Positives = 63/133 (47%) Frame = -3 Query: 480 FNKAMALVSEDFLYFTRYYINTWFPARNLVRKAIDNRFQIHKSGEIVVFNERFPWKYHLF 301 F K+ + ED+ + R+Y N W A +++ + F + +V E ++Y + Sbjct: 298 FRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIK 357 Query: 300 DLEQELGIEPQIKYVHSMINQNLGEFKLFQLIL*VLFLGKHYIVHGVESVTIISVQSLGL 121 ++ + + I +N ++ +F + +L + V+ + ++++H + + VQ + + Sbjct: 358 NV-RSMVIWLVDTPAGLKLNNDICKF-IMKLSVWVIDVWSNFLLHCLPWTPFL-VQVVAI 414 Query: 120 KDVFSATAMVSLV 82 A+ M++L+ Sbjct: 415 SGFGGASLMIALI 427 >SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 28.7 bits (61), Expect = 0.85 Identities = 13/31 (41%), Positives = 20/31 (64%) Frame = +3 Query: 78 LPPMKPLLWQKIHPSIPETELRLSSRIPRHA 170 + P+ PLL Q+I S+P++ L S + P HA Sbjct: 794 ISPITPLLAQEIWQSLPDSYLNSSYQTPFHA 824 >SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1150 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/64 (18%), Positives = 29/64 (45%) Frame = -3 Query: 669 NEDLKLIYKKVYESFIQGIDAIDNGIPMTEEQPKYDIHTHLSNRVKRLEPRMELTQETNV 490 N+ L+ + K Y ++ ++ D + K + + N++ L + ++ N+ Sbjct: 510 NDPLEEKWSKFYVDLLEKLEKFDEALEFASAISKGKVSIYNKNKILELSKKTAKSKVWNL 569 Query: 489 DEFF 478 D+FF Sbjct: 570 DDFF 573 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 27.9 bits (59), Expect = 1.5 Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 4/48 (8%) Frame = +3 Query: 15 LFHSLASRLAMAIFKAPSNVVLPPMKP----LLWQKIHPSIPETELRL 146 L++S A +A++ + PSNV +PP P W I I +L L Sbjct: 338 LYNSRARSVALSEYHQPSNVFVPPEDPDLPAFFWDPIINPITSRQLTL 385 >SPAC7D4.09c |||steroid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 274 Score = 26.2 bits (55), Expect = 4.5 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = +3 Query: 492 HWFPELIPFWVQVFSLCYLSEYECHISVVPLSLEFRY 602 HW L+ WV + S Y C + SL+FR+ Sbjct: 231 HWLIWLVLGWVLCNMVAISSSYACTVKNKEQSLDFRW 267 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = -1 Query: 698 SKEESTSFDQMKT*S*FTRKFMNLSFKGLMLLITEF 591 SK+ Q++ KFMN+ FKGL +I+ F Sbjct: 340 SKDHLKVLQQLRPLIDIVEKFMNVYFKGLSNIISSF 375 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 26.2 bits (55), Expect = 4.5 Identities = 7/16 (43%), Positives = 10/16 (62%) Frame = +3 Query: 495 WFPELIPFWVQVFSLC 542 W P LI FW+ ++C Sbjct: 1504 WIPHLIYFWISTLAMC 1519 >SPBC31E1.02c |pmr1||P-type ATPase, calcium transporting Pmr1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 899 Score = 25.8 bits (54), Expect = 6.0 Identities = 21/86 (24%), Positives = 38/86 (44%), Gaps = 1/86 (1%) Frame = -3 Query: 303 FDLEQELGIEPQIKYVHSMINQNLGEFKLFQLIL*VLFLGKHYIVHGVESVTIISVQSLG 124 FD+ L + K V + + ++F + + +G+ +V+ I +++G Sbjct: 801 FDMFNALACRSETKSVFKL---GIFSNRMFNIAVGGSLIGQALVVYASPFQRIFQTEAIG 857 Query: 123 LKDVFSATAMVSLVVIPHW-REL*KW 49 LKDV A S V+ W E+ KW Sbjct: 858 LKDVLILLACTSSVL---WVDEIRKW 880 >SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/24 (45%), Positives = 15/24 (62%), Gaps = 2/24 (8%) Frame = +1 Query: 577 FLCHWN--SVINSINPLNERFINF 642 F CH N SVIN + ++ F+NF Sbjct: 216 FTCHQNAFSVINEVRNFSQGFVNF 239 >SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 703 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/43 (30%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = +1 Query: 253 MHIFDLRLNPQFLFQIKQMIFPRKPFIE-YHNLS*LVYLKTII 378 MH + L Q L+Q K + P+K ++ +H ++ +VY ++ I Sbjct: 206 MHKQESFLRKQLLYQCKSCVKPKKILVDLFHRIN-IVYFRSSI 247 >SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 25.4 bits (53), Expect = 7.9 Identities = 31/115 (26%), Positives = 52/115 (45%), Gaps = 6/115 (5%) Frame = -3 Query: 672 PNEDLKLIYKKVYESF---IQGIDAIDNGIPMTEEQPKYDIHTHLSNRVKRLEPRMELTQ 502 P + KL YKK+YE + I+ + P +EE + +++S++ K +E + Sbjct: 268 PKPENKLDYKKLYEKYSALIEKYPIVFIEDPFSEED--WGAFSYMSSKTK-VEVIADDLT 324 Query: 501 ETNVDEFFNKAMALVSEDFLYFTRYYINT---WFPARNLVRKAIDNRFQIHKSGE 346 TNV +KA+ L + L I + A N+ +KA H+SGE Sbjct: 325 VTNVKR-LSKAIELKCANALLVKINQIGSLSETIDAANMAKKAGWGLMVSHRSGE 378 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,103,046 Number of Sequences: 5004 Number of extensions: 69516 Number of successful extensions: 195 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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