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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0732.Seq
         (698 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g41970.1 68418.m05110 expressed protein contains Pfam profile...   120   7e-28
At3g49320.1 68416.m05392 expressed protein contains Pfam profile...   112   2e-25
At5g01030.2 68418.m00006 expressed protein                             31   0.56 
At5g01030.1 68418.m00005 expressed protein                             31   0.56 
At2g27315.1 68415.m03283 hypothetical protein                          31   0.97 
At3g19190.1 68416.m02436 expressed protein                             30   1.3  

>At5g41970.1 68418.m05110 expressed protein contains Pfam profile
           PF03690: Uncharacterised protein family (UPF0160)
          Length = 346

 Score =  120 bits (290), Expect = 7e-28
 Identities = 61/141 (43%), Positives = 93/141 (65%), Gaps = 5/141 (3%)
 Frame = -3

Query: 663 DLKLIYKKVYESFIQGIDAIDNGIPM--TEEQPKYDIHTHLSNRVKRLEPRM---ELTQE 499
           D+  ++  VY+SF++ IDA+DNGI    T++ P+Y  +THLS RV RL       + +QE
Sbjct: 130 DVLRLFLAVYKSFMEAIDAVDNGINRYDTDQPPRYVNNTHLSPRVGRLNLDWIDPDQSQE 189

Query: 498 TNVDEFFNKAMALVSEDFLYFTRYYINTWFPARNLVRKAIDNRFQIHKSGEIVVFNERFP 319
              +E F +AMAL  ++FL   +++  +W PAR++V + ++ RF+   SGEI++ +   P
Sbjct: 190 KE-NEAFQRAMALAGKEFLESVQFHARSWLPARSIVMQCLEERFKTDPSGEIMILDRFCP 248

Query: 318 WKYHLFDLEQELGIEPQIKYV 256
           WK HLF+LEQE+ IEP IKYV
Sbjct: 249 WKLHLFELEQEMKIEPLIKYV 269



 Score = 82.2 bits (194), Expect = 3e-16
 Identities = 36/72 (50%), Positives = 48/72 (66%)
 Frame = -2

Query: 250 NDKPKSWRVQAVPIDPMSFVLRKALHSAWRGIRDDNLSSVSGIEGCIFCHSNGFIGGNTT 71
           +++ K WRVQAV + P  F  RK L   WRG+RD+ LS  + I GC+F H +GFIGGN +
Sbjct: 273 DERAKQWRVQAVAVAPDRFENRKPLPEKWRGLRDEELSKAAEIPGCVFVHMSGFIGGNQS 332

Query: 70  LEGALKMAIASL 35
            +GAL MA  +L
Sbjct: 333 YDGALSMAQTAL 344


>At3g49320.1 68416.m05392 expressed protein contains Pfam profile
           PF03690: Uncharacterised protein family (UPF0160)
          Length = 354

 Score =  112 bits (269), Expect = 2e-25
 Identities = 54/140 (38%), Positives = 90/140 (64%), Gaps = 4/140 (2%)
 Frame = -3

Query: 663 DLKLIYKKVYESFIQGIDAIDNGIPM--TEEQPKYDIHTHLSNRVKRLE-PRMELTQET- 496
           D+  ++  VY++FI+ +DA+DNGI    T++ P+Y  +T L +R+ RL    +E  Q + 
Sbjct: 138 DVFRLFLAVYKNFIEAVDALDNGIHQYDTDQPPRYVNNTSLGHRIGRLNLDWIEPDQSSA 197

Query: 495 NVDEFFNKAMALVSEDFLYFTRYYINTWFPARNLVRKAIDNRFQIHKSGEIVVFNERFPW 316
             DE F++AM L   +FL    ++  +W PAR++V + +  R+ I  SGEI+  +++ PW
Sbjct: 198 KEDEAFHRAMELAGSEFLECVHFHAKSWLPARSIVMECLAKRYDIDSSGEIMKLSKQCPW 257

Query: 315 KYHLFDLEQELGIEPQIKYV 256
           K H+F+LE+E+ I+P IKYV
Sbjct: 258 KLHIFELEEEMKIDPPIKYV 277



 Score = 83.8 bits (198), Expect = 1e-16
 Identities = 39/74 (52%), Positives = 49/74 (66%)
 Frame = -2

Query: 250 NDKPKSWRVQAVPIDPMSFVLRKALHSAWRGIRDDNLSSVSGIEGCIFCHSNGFIGGNTT 71
           +D+ ++WR+QAV + P  F  RKAL  AWRG+  + LS  S I  C+F H +GFIG N T
Sbjct: 281 DDRSENWRIQAVSVSPERFESRKALPLAWRGLEKEKLSEESSIPRCVFVHMSGFIGANQT 340

Query: 70  LEGALKMAIASLEA 29
            EGAL MA ASL A
Sbjct: 341 YEGALAMARASLMA 354


>At5g01030.2 68418.m00006 expressed protein 
          Length = 744

 Score = 31.5 bits (68), Expect = 0.56
 Identities = 22/76 (28%), Positives = 34/76 (44%)
 Frame = +2

Query: 77  ITTNETIAVAENTSFNPRD*TEIIVTDSTPCTM*CFPKNKTHRINWNSLNSPRFWFIIEC 256
           + +N+T+    N SF   D  E+   DS+P       K   +R+ + S  SP   F +  
Sbjct: 657 VLSNKTVLHKFNQSFTLFD-QEVSEQDSSPALAMTELKTGIYRVEFGSFVSPLQAFFVCV 715

Query: 257 TYLICGSIPNSCSKSN 304
           T L C S   +  KS+
Sbjct: 716 TVLTCASKAKTTGKSS 731


>At5g01030.1 68418.m00005 expressed protein 
          Length = 744

 Score = 31.5 bits (68), Expect = 0.56
 Identities = 22/76 (28%), Positives = 34/76 (44%)
 Frame = +2

Query: 77  ITTNETIAVAENTSFNPRD*TEIIVTDSTPCTM*CFPKNKTHRINWNSLNSPRFWFIIEC 256
           + +N+T+    N SF   D  E+   DS+P       K   +R+ + S  SP   F +  
Sbjct: 657 VLSNKTVLHKFNQSFTLFD-QEVSEQDSSPALAMTELKTGIYRVEFGSFVSPLQAFFVCV 715

Query: 257 TYLICGSIPNSCSKSN 304
           T L C S   +  KS+
Sbjct: 716 TVLTCASKAKTTGKSS 731


>At2g27315.1 68415.m03283 hypothetical protein
          Length = 120

 Score = 30.7 bits (66), Expect = 0.97
 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
 Frame = -2

Query: 142 LSSVSGIEGCIFCHSNGFIGGN-TTLEGALKMAIASLEAN 26
           +SS+S +EGC+   S     G    +EGA   A ++L+AN
Sbjct: 50  MSSLSNVEGCVLEISKSIFSGKFENIEGACCKAFSALDAN 89


>At3g19190.1 68416.m02436 expressed protein
          Length = 1861

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 12/25 (48%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
 Frame = -3

Query: 321  PWKYHLFDLEQEL-GIEPQIKYVHS 250
            PWK++L   E+++ GIE Q+K+VH+
Sbjct: 1592 PWKHNLLITEEQMQGIELQLKHVHA 1616


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,846,901
Number of Sequences: 28952
Number of extensions: 346915
Number of successful extensions: 849
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 846
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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