BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0718.Seq (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18.14c |rpp0||60S acidic ribosomal protein Rpp0 |Schizosacch... 69 3e-13 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 28 0.68 SPAC823.13c |||mitochondrial inner membrane protein|Schizosaccha... 27 1.2 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 1.2 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 2.1 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 26 2.7 SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 26 2.7 SPBC3B9.15c |scp1||sterol regulatory element binding protein Scp... 26 3.6 SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2... 25 4.8 SPAC13C5.05c |||N-acetylglucosamine-phosphate mutase |Schizosacc... 25 6.3 SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 25 8.4 >SPCC18.14c |rpp0||60S acidic ribosomal protein Rpp0 |Schizosaccharomyces pombe|chr 3|||Manual Length = 312 Score = 69.3 bits (162), Expect = 3e-13 Identities = 43/142 (30%), Positives = 67/142 (47%), Gaps = 1/142 (0%) Frame = +2 Query: 68 KSNYFVKIIQLLDEYPKCFIVGADNVGSQQMQQIRISLRGSSIALMGKNTMMRKAIKDHL 247 K+ YF K+ L ++Y F+V DNV SQQM +R LRG++ +MGKNTM+R+A++ + Sbjct: 8 KAQYFEKLRSLFEKYNSLFVVNIDNVSSQQMHTVRKQLRGTAELIMGKNTMIRRAMRGII 67 Query: 248 DNIQPSRNCCHTSRATLASCSPAETXLRS*QT-VGEQSPGSSSSRCHCPIVSRHSRHNTG 424 +++ R + +T + + P+ NTG Sbjct: 68 NDMPELERLLPVVRGNVGFVFTNADLKEVRETIIANVIAAPARPNAIAPLDVFVPAGNTG 127 Query: 425 LGPEKTXXLQALFILPKXQKGT 490 + P KT QAL I K +GT Sbjct: 128 MEPGKTSFFQALGIPTKITRGT 149 Score = 41.9 bits (94), Expect = 5e-05 Identities = 18/26 (69%), Positives = 21/26 (80%) Frame = +1 Query: 256 PALEKLLPHIKGNVGFVFTRGDLVEV 333 P LE+LLP ++GNVGFVFT DL EV Sbjct: 71 PELERLLPVVRGNVGFVFTNADLKEV 96 Score = 41.9 bits (94), Expect = 5e-05 Identities = 21/55 (38%), Positives = 28/55 (50%) Frame = +3 Query: 333 RDKLLENKVQAPARPGAIAPLSVVIPATTPASVQRKPXXYKLFSSYQKXKRGLIE 497 R+ ++ N + APARP AIAPL V +PA K ++ K RG IE Sbjct: 97 RETIIANVIAAPARPNAIAPLDVFVPAGNTGMEPGKTSFFQALGIPTKITRGTIE 151 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 28.3 bits (60), Expect = 0.68 Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 4/46 (8%) Frame = +1 Query: 349 RTKSRLQLVPVPLPHCQSSFPPQHRPRSR----ENLXLTSSFHPTK 474 RT +R + + VP P ++SFP H PRSR +N + +S+H K Sbjct: 137 RTSTRKRSLTVPTP--RTSFP--HHPRSRRFSLKNAAIATSYHKFK 178 >SPAC823.13c |||mitochondrial inner membrane protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 27.5 bits (58), Expect = 1.2 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = -2 Query: 306 HEANVALDVWQQFLEGWMLSRWSLMALRIIVFFPMSAILEPR 181 HE V D +Q W W LM + +++F + ILEPR Sbjct: 151 HEEQVWSDKIRQ-ASTW--GTWGLMGINVVLFVVVQLILEPR 189 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 1.2 Identities = 14/45 (31%), Positives = 23/45 (51%) Frame = +2 Query: 290 ATLASCSPAETXLRS*QTVGEQSPGSSSSRCHCPIVSRHSRHNTG 424 +++AS +PA T + ++ E+ S+SSR H HN G Sbjct: 1270 SSMASAAPARTSVSRSKSKAERHETSTSSRKSSKSGEHHHHHNEG 1314 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 26.6 bits (56), Expect = 2.1 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +1 Query: 298 GFVFTRGDLVEVVTNCWRTKSRLQLVPVPL 387 GFV T +++ + T CW ++ QLV PL Sbjct: 548 GFVATFSEML-IQTQCWHLSAQTQLVSNPL 576 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 26.2 bits (55), Expect = 2.7 Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = -3 Query: 383 GTGTSWSL-DFVLQQFVTTSTRSPRVNTKPTLPLMCGNSFSRAGCCPG 243 G G WS+ +QF+ R P VN++P P+ S ++ G G Sbjct: 197 GKGHFWSIRPGHEEQFLKLKLRKPGVNSRPAPPVQDVTSSTKYGSSTG 244 >SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces pombe|chr 3|||Manual Length = 920 Score = 26.2 bits (55), Expect = 2.7 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = +1 Query: 391 HCQSSFPPQHRPRSRENLXLTSSF 462 H SSF QH P +R+ + TS F Sbjct: 91 HLDSSFSSQHSPDNRKRMSSTSVF 114 >SPBC3B9.15c |scp1||sterol regulatory element binding protein Scp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1086 Score = 25.8 bits (54), Expect = 3.6 Identities = 15/56 (26%), Positives = 28/56 (50%) Frame = +2 Query: 104 DEYPKCFIVGADNVGSQQMQQIRISLRGSSIALMGKNTMMRKAIKDHLDNIQPSRN 271 DE+ +V ++ G Q+ + I + G+ IAL+ K + IK + + P+ N Sbjct: 698 DEWKNFELVVVESSGEIQVFSLTIEIEGADIALVEKFQLSSPIIKS-ISIVSPTAN 752 >SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 202 Score = 25.4 bits (53), Expect = 4.8 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = +2 Query: 203 MGKNTMMRKAIKDHLDNIQPSRNCCHTSRATLA 301 +GK+T++++ +KDH D + + HT+R A Sbjct: 29 VGKSTLLKRLLKDHGDKL--GFSVSHTTRTPRA 59 >SPAC13C5.05c |||N-acetylglucosamine-phosphate mutase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 25.0 bits (52), Expect = 6.3 Identities = 11/28 (39%), Positives = 20/28 (71%), Gaps = 4/28 (14%) Frame = -3 Query: 359 DFVLQQFVTTSTRSPRV----NTKPTLP 288 DF+++QF+T +T P+V +T+P+ P Sbjct: 91 DFLIKQFLTPTTCQPKVIIGYDTRPSSP 118 >SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein 3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1385 Score = 24.6 bits (51), Expect = 8.4 Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 1/76 (1%) Frame = +2 Query: 152 QQMQQIRISLRGSSI-ALMGKNTMMRKAIKDHLDNIQPSRNCCHTSRATLASCSPAETXL 328 +Q+Q+ S S I +L GK ++ + + IQPS + + +A PA T + Sbjct: 504 RQIQKAESSEDTSEIESLQGKLSLPQ--VSSTQQEIQPSSSVPEAASNEIAEKEPAVTAI 561 Query: 329 RS*QTVGEQSPGSSSS 376 S E++P SSS Sbjct: 562 ESITERKEEAPVISSS 577 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,184,583 Number of Sequences: 5004 Number of extensions: 43195 Number of successful extensions: 142 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 141 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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