Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0718.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09200.1 68416.m01094 60S acidic ribosomal protein P0 (RPP0B)... 82 2e-16
At3g11250.1 68416.m01368 60S acidic ribosomal protein P0 (RPP0C)... 81 3e-16
At2g40010.1 68415.m04916 60S acidic ribosomal protein P0 (RPP0A) 78 4e-15
At4g38530.1 68417.m05454 phosphoinositide-specific phospholipase... 29 2.3
At5g13260.1 68418.m01523 expressed protein 28 3.0
At1g53800.1 68414.m06123 expressed protein 28 3.0
At4g09560.1 68417.m01571 protease-associated zinc finger (C3HC4-... 27 5.3
At5g26742.1 68418.m03161 DEAD box RNA helicase (RH3) nearly iden... 27 7.0
At1g51860.1 68414.m05846 leucine-rich repeat protein kinase, put... 27 7.0
At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8 ... 27 9.3
At5g03800.1 68418.m00347 exostosin family protein / pentatricope... 27 9.3
>At3g09200.1 68416.m01094 60S acidic ribosomal protein P0 (RPP0B)
similar to putative 60S acidic ribosomal protein P0
GB:P50346 [Glycine max]
Length = 320
Score = 81.8 bits (193), Expect = 2e-16
Identities = 54/153 (35%), Positives = 75/153 (49%), Gaps = 3/153 (1%)
Frame = +2
Query: 41 MGREDKATWKSNYFVKIIQLLDEYPKCFIVGADNVGSQQMQQIRISLRGSSIALMGKNTM 220
M + KA K Y K+ QL+DEY + +V ADNVGS Q+Q IR LRG S+ LMGKNTM
Sbjct: 1 MVKATKAEKKIAYDTKLCQLIDEYTQILVVAADNVGSTQLQNIRKGLRGDSVVLMGKNTM 60
Query: 221 MRKAIKDHLDNIQPSR--NCCHTSRATLASCSPAETXLRS*QTVGEQSPGSSSS-RCHCP 391
M+++++ H +N + N + + + V + G+ + P
Sbjct: 61 MKRSVRIHSENTGNTAILNLLPLLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAP 120
Query: 392 IVSRHSRHNTGLGPEKTXXLQALFILPKXQKGT 490
I NTGL P +T Q L I K KGT
Sbjct: 121 IDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGT 153
Score = 35.9 bits (79), Expect = 0.015
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +1
Query: 259 ALEKLLPHIKGNVGFVFTRGDLVEV 333
A+ LLP ++GNVG +FT+GDL EV
Sbjct: 76 AILNLLPLLQGNVGLIFTKGDLKEV 100
Score = 29.9 bits (64), Expect = 1.00
Identities = 14/54 (25%), Positives = 28/54 (51%)
Frame = +3
Query: 336 DKLLENKVQAPARPGAIAPLSVVIPATTPASVQRKPXXYKLFSSYQKXKRGLIE 497
+++ + KV APAR G +AP+ VV+ + +++ + K +G +E
Sbjct: 102 EEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVE 155
>At3g11250.1 68416.m01368 60S acidic ribosomal protein P0 (RPP0C)
similar to 60S acidic ribosomal protein P0 GI:2088654
[Arabidopsis thaliana]
Length = 323
Score = 81.4 bits (192), Expect = 3e-16
Identities = 54/153 (35%), Positives = 75/153 (49%), Gaps = 3/153 (1%)
Frame = +2
Query: 41 MGREDKATWKSNYFVKIIQLLDEYPKCFIVGADNVGSQQMQQIRISLRGSSIALMGKNTM 220
M + KA K Y K+ QL+DEY + +V ADNVGS Q+Q IR LRG S+ LMGKNTM
Sbjct: 1 MVKATKAEKKIAYDTKLCQLIDEYTQILVVAADNVGSTQLQNIRKGLRGDSVVLMGKNTM 60
Query: 221 MRKAIKDHLDNIQPSR--NCCHTSRATLASCSPAETXLRS*QTVGEQSPGSSSS-RCHCP 391
M+++++ H +N + N + + + V + G+ + P
Sbjct: 61 MKRSVRIHSENSGNTAILNLLPLLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAP 120
Query: 392 IVSRHSRHNTGLGPEKTXXLQALFILPKXQKGT 490
I NTGL P +T Q L I K KGT
Sbjct: 121 IDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGT 153
Score = 35.9 bits (79), Expect = 0.015
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +1
Query: 259 ALEKLLPHIKGNVGFVFTRGDLVEV 333
A+ LLP ++GNVG +FT+GDL EV
Sbjct: 76 AILNLLPLLQGNVGLIFTKGDLKEV 100
Score = 29.9 bits (64), Expect = 1.00
Identities = 14/54 (25%), Positives = 28/54 (51%)
Frame = +3
Query: 336 DKLLENKVQAPARPGAIAPLSVVIPATTPASVQRKPXXYKLFSSYQKXKRGLIE 497
+++ + KV APAR G +AP+ VV+ + +++ + K +G +E
Sbjct: 102 EEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVE 155
>At2g40010.1 68415.m04916 60S acidic ribosomal protein P0 (RPP0A)
Length = 317
Score = 77.8 bits (183), Expect = 4e-15
Identities = 53/148 (35%), Positives = 72/148 (48%), Gaps = 3/148 (2%)
Frame = +2
Query: 56 KATWKSNYFVKIIQLLDEYPKCFIVGADNVGSQQMQQIRISLRGSSIALMGKNTMMRKAI 235
KA K Y K+ QLL+EY + +V ADNVGS Q+Q IR LRG S+ LMGKNTMM++++
Sbjct: 7 KAEKKIVYDSKLCQLLNEYSQILVVAADNVGSTQLQNIRKGLRGDSVVLMGKNTMMKRSV 66
Query: 236 KDHLDNI--QPSRNCCHTSRATLASCSPAETXLRS*QTVGEQSPGSSSS-RCHCPIVSRH 406
+ H D Q + + + + V + G+ + PI
Sbjct: 67 RIHADKTGNQAFLSLLPLLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVV 126
Query: 407 SRHNTGLGPEKTXXLQALFILPKXQKGT 490
NTGL P +T Q L I K KGT
Sbjct: 127 QPGNTGLDPSQTSFFQVLNIPTKINKGT 154
Score = 35.1 bits (77), Expect = 0.026
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +1
Query: 259 ALEKLLPHIKGNVGFVFTRGDLVEV 333
A LLP ++GNVG +FT+GDL EV
Sbjct: 77 AFLSLLPLLQGNVGLIFTKGDLKEV 101
Score = 29.9 bits (64), Expect = 1.00
Identities = 14/54 (25%), Positives = 28/54 (51%)
Frame = +3
Query: 336 DKLLENKVQAPARPGAIAPLSVVIPATTPASVQRKPXXYKLFSSYQKXKRGLIE 497
+++ + KV APAR G +AP+ VV+ + +++ + K +G +E
Sbjct: 103 EEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVE 156
>At4g38530.1 68417.m05454 phosphoinositide-specific phospholipase C
nearly identical to phosphoinositide-specific
phospholipase C GI:557880 from [Arabidopsis thaliana];
contains Pfam profile: PF00388
phosphatidylinositol-specific phospholipase C
Length = 526
Score = 28.7 bits (61), Expect = 2.3
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1
Query: 262 GLDVVQVVFDGFAHHSVFSH 203
GLD VQ +F HH+VF H
Sbjct: 20 GLDYVQDIFHSVKHHNVFHH 39
>At5g13260.1 68418.m01523 expressed protein
Length = 537
Score = 28.3 bits (60), Expect = 3.0
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -3
Query: 200 ARYWSHVARYGSAASVASPRYRH 132
ARYW +RYG + +A+ +Y +
Sbjct: 330 ARYWGLASRYGICSDIATSKYEY 352
>At1g53800.1 68414.m06123 expressed protein
Length = 568
Score = 28.3 bits (60), Expect = 3.0
Identities = 16/37 (43%), Positives = 19/37 (51%)
Frame = +1
Query: 223 AQSHQRPPGQHPALEKLLPHIKGNVGFVFTRGDLVEV 333
A++H P G H EK GNV + RG LVEV
Sbjct: 528 AENHPLPNGYHGIDEKAASLESGNVTKKWVRGRLVEV 564
>At4g09560.1 68417.m01571 protease-associated zinc finger
(C3HC4-type RING finger) family protein contains Pfam
domain, PF02225: protease-associated (PA) domain and
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger); similar to ReMembR-H2 protein JR702
[Arabidopsis thaliana] gi|6942149|gb|AAF32326
Length = 431
Score = 27.5 bits (58), Expect = 5.3
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = -2
Query: 309 EHEANVALDVWQQFLEGWMLSRWSLMALRIIVFFPMSAIL 190
E+ +VW + + S WS+MA+ I MSA+L
Sbjct: 127 EYAGRTDFEVW--LMPSFETSAWSIMAISFISLLAMSAVL 164
>At5g26742.1 68418.m03161 DEAD box RNA helicase (RH3) nearly
identical to RNA helicase [Arabidopsis thaliana]
GI:3775987; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain, PF00098: Zinc knuckle
Length = 747
Score = 27.1 bits (57), Expect = 7.0
Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -3
Query: 431 DRG--RCCGGNDD*QWGNGTGTSWSLDFVLQQFVTTSTRSPRVNTKPTLPLMCGNSFSRA 258
DRG R GG G + S D+++ ++S+R+P + +CG S RA
Sbjct: 679 DRGSRRSSGGGSSWSRGGSSSRGSSDDWLIGGRSSSSSRAPSRESFGGSCFICGKSGHRA 738
Query: 257 GCCP 246
CP
Sbjct: 739 TDCP 742
>At1g51860.1 68414.m05846 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 890
Score = 27.1 bits (57), Expect = 7.0
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 277 PHIKGNVGFVFTRGDLVEVV 336
PHI VGF+ T+GD+ +V
Sbjct: 792 PHINDWVGFMLTKGDIKSIV 811
>At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8
WD-40 repeats (PF00400) (2 weak)
Length = 1108
Score = 26.6 bits (56), Expect = 9.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 205 GKKHYDAQSHQRPPGQHPALEKLLPHIKGNVGFVFT 312
G+KH+ + H P + + PH K N+ F+F+
Sbjct: 485 GRKHFTFEGHDAP------VYSICPHYKENIQFIFS 514
>At5g03800.1 68418.m00347 exostosin family protein /
pentatricopeptide (PPR) repeat-containing protein
contains Pfam profiles: PF03016 exostosin family,
PF01535 PPR repeat
Length = 1388
Score = 26.6 bits (56), Expect = 9.3
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +1
Query: 388 PHCQSSFPPQHRPRSRENLXL 450
PH S PP HRP+ +L L
Sbjct: 13 PHIPPSIPPNHRPKLLSSLSL 33
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,855,921
Number of Sequences: 28952
Number of extensions: 249768
Number of successful extensions: 748
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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