BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0709.Seq (409 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 35 0.006 SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 26 2.6 >SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity factor complex subunit Ysh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 34.7 bits (76), Expect = 0.006 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = +3 Query: 252 QSCIMLEFKGKXIMLDCGXHPGLXG 326 +SC ++++KGK +MLD G HP G Sbjct: 50 RSCHVIQYKGKTVMLDAGVHPAYTG 74 Score = 25.4 bits (53), Expect = 3.4 Identities = 19/57 (33%), Positives = 22/57 (38%) Frame = +1 Query: 208 DQLTIRPLGAGQEVGSHASCWSSKGKX*CWTVAXILDXLXWTALPFIDLXEAD*VDL 378 D L LGAG EVG KGK +ALPF D + VD+ Sbjct: 35 DLLEFINLGAGNEVGRSCHVIQYKGKTVMLDAGVHPAYTGLSALPFFDEFDLSTVDV 91 >SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 703 Score = 25.8 bits (54), Expect = 2.6 Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = -2 Query: 225 SYCQLIAFLRRHWISAFSWICSHFVYLXIFIY-RDLTNSL 109 SY L A L + WIS +S +C ++ + F++ +DL + Sbjct: 4 SYLSLAARLSQCWISPWS-LCCLYILMQFFLFTKDLNTKI 42 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,393,616 Number of Sequences: 5004 Number of extensions: 23809 Number of successful extensions: 49 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 47 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 49 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 140222766 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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