Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0708.Seq
         (617 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ667188-1|ABG75740.1|  383|Apis mellifera histamine-gated chlor...    23   1.8  
DQ026037-1|AAY87896.1|  431|Apis mellifera nicotinic acetylcholi...    23   3.2  
AY540846-1|AAS48080.1|  541|Apis mellifera neuronal nicotinic ac...    22   4.2  
DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholi...    22   5.5  

>DQ667188-1|ABG75740.1|  383|Apis mellifera histamine-gated chloride
           channel protein.
          Length = 383

 Score = 23.4 bits (48), Expect = 1.8
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = +1

Query: 475 SWRNSRRGPPRCPSQQLRSLNGEW 546
           SWR+SR   P   S+  R L+ +W
Sbjct: 60  SWRDSRLRLPENMSEDYRILDVDW 83


>DQ026037-1|AAY87896.1|  431|Apis mellifera nicotinic acetylcholine
           receptor alpha9subunit protein.
          Length = 431

 Score = 22.6 bits (46), Expect = 3.2
 Identities = 15/45 (33%), Positives = 20/45 (44%)
 Frame = -1

Query: 254 MSQCKPY*GDTANGSIYQFWFLRSYSVTWITVVILELIHAIRTLT 120
           M  C P   DT    +Y+F   R Y +   T VI  +   + TLT
Sbjct: 230 MYACCP--NDTYPMIVYEFSISRHYGILHATYVIPAVTMMLLTLT 272


>AY540846-1|AAS48080.1|  541|Apis mellifera neuronal nicotinic
           acetylcholine receptorApisa2 subunit protein.
          Length = 541

 Score = 22.2 bits (45), Expect = 4.2
 Identities = 9/29 (31%), Positives = 17/29 (58%)
 Frame = -3

Query: 255 DVSVQAVLRRYREWLNISVLVP*ILLSYL 169
           D+     LRR   +  ++++VP + +SYL
Sbjct: 224 DIFFNITLRRKTLFYTVNLIVPCVSISYL 252


>DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholine
           receptor alpha3subunit protein.
          Length = 566

 Score = 21.8 bits (44), Expect = 5.5
 Identities = 8/17 (47%), Positives = 11/17 (64%)
 Frame = -3

Query: 63  PKSLILMNLDNFCRSHG 13
           P+ L  +NL + CR HG
Sbjct: 430 PRELEAVNLGSACRIHG 446


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 157,218
Number of Sequences: 438
Number of extensions: 3156
Number of successful extensions: 8
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18337950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)