Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0701.Seq
(617 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04620.2 68418.m00464 aminotransferase class I and II family ... 63 2e-10
At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2) id... 60 2e-09
At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative... 58 4e-09
At4g36480.1 68417.m05180 aminotransferase class I and II family ... 50 1e-06
At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative... 47 9e-06
At5g04620.1 68418.m00465 aminotransferase class I and II family ... 46 3e-05
At1g16960.1 68414.m02059 expressed protein 32 0.26
At1g22930.1 68414.m02866 T-complex protein 11 contains Pfam PF05... 32 0.35
At5g27230.1 68418.m03248 expressed protein ; expression support... 29 2.5
At1g72210.1 68414.m08349 basic helix-loop-helix (bHLH) family pr... 29 3.3
At5g43175.1 68418.m05270 basic helix-loop-helix (bHLH) family pr... 28 4.3
At2g37080.1 68415.m04550 myosin heavy chain-related low similari... 28 4.3
At5g52580.1 68418.m06529 hypothetical protein 28 5.7
At3g28300.1 68416.m03535 integrin-related protein 14a identical ... 28 5.7
At3g28290.1 68416.m03533 integrin-related protein 14a identical ... 28 5.7
At1g50660.1 68414.m05696 expressed protein similar to liver stag... 28 5.7
At1g24340.1 68414.m03070 monooxygenase family protein similar to... 27 7.5
At3g20350.1 68416.m02578 expressed protein 27 10.0
>At5g04620.2 68418.m00464 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 476
Score = 62.9 bits (146), Expect = 2e-10
Identities = 41/109 (37%), Positives = 55/109 (50%), Gaps = 14/109 (12%)
Frame = +2
Query: 248 LIDHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDA 427
L HP + AA +K+YG G ICG + H+ LE+ L+Q +ED ++ + F A
Sbjct: 115 LSSHPTISNAAANAVKEYGMGPKGSALICGYTTYHRLLESSLAQLKKKEDCLVCPTGFAA 174
Query: 428 N------AGLFESMLTPED--------AVFSDALNHASIIDGSGYARRR 532
N G S+L A+FSDALNHASIIDG A R+
Sbjct: 175 NMAAMVAIGSVASLLAASGKPLKNEKVAIFSDALNHASIIDGVRLAERQ 223
>At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2)
identical to serine palmitoyltransferase [Arabidopsis
thaliana] GI:9309380; similar to serine
palmitoyltransferase from Solanum tuberosum
[GI:4995890], Homo sapiens [SP|O15270], Mus musculus
[SP|P97363]; contains Pfam profile PF00155:
aminotransferase, classes I and II
Length = 489
Score = 59.7 bits (138), Expect = 2e-09
Identities = 26/77 (33%), Positives = 46/77 (59%)
Frame = +2
Query: 284 EGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPE 463
E LKK+ A S R GT S+H ELE +++F G+ +++G + N+ + ++
Sbjct: 126 ESLKKFSASTCSSRVDAGTTSVHAELEECVTRFVGKPAAVVFGMGYATNSAIIPVLIGKG 185
Query: 464 DAVFSDALNHASIIDGS 514
+ SD+LNH+SI++G+
Sbjct: 186 GLIISDSLNHSSIVNGA 202
>At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 489
Score = 58.4 bits (135), Expect = 4e-09
Identities = 25/77 (32%), Positives = 46/77 (59%)
Frame = +2
Query: 284 EGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPE 463
E LKK+ A S R GT S+H ELE+ ++++ G+ +++G + N+ + ++
Sbjct: 126 ESLKKFSASTCSSRVDAGTTSVHAELEDCVAKYVGQPAAVIFGMGYATNSAIIPVLIGKG 185
Query: 464 DAVFSDALNHASIIDGS 514
+ SD+LNH SI++G+
Sbjct: 186 GLIISDSLNHTSIVNGA 202
>At4g36480.1 68417.m05180 aminotransferase class I and II family
protein similar to Serine palmitoyltransferase 1 (EC
2.3.1.50) from Homo sapiens [SP|O15269], Mus musculus
[SP|O35704], Cricetulus griseus [SP|O54695]
Length = 482
Score = 50.0 bits (114), Expect = 1e-06
Identities = 28/93 (30%), Positives = 42/93 (45%)
Frame = +2
Query: 248 LIDHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDA 427
LI H +++E+ L+KYG G R GT +H + E R+S+F G D+ILY
Sbjct: 122 LIGHEKLLESCTSALEKYGVGSCGPRGFYGTIDVHLDCETRISKFLGTPDSILYSYGLST 181
Query: 428 NAGLFESMLTPEDAVFSDALNHASIIDGSGYAR 526
D + +D H I +G +R
Sbjct: 182 MFSTIPCFCKKGDVIVADEGVHWGIQNGLQLSR 214
>At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 350
Score = 47.2 bits (107), Expect = 9e-06
Identities = 19/62 (30%), Positives = 38/62 (61%)
Frame = +2
Query: 329 ICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPEDAVFSDALNHASIID 508
+ GT ++H ELE +++F G+ +++G + N+ + ++ + SD+LNH SII+
Sbjct: 5 LSGTTAVHGELEECVAKFVGKPAAVVFGMGYLTNSAIISVLIGKGGLIISDSLNHTSIIN 64
Query: 509 GS 514
G+
Sbjct: 65 GA 66
>At5g04620.1 68418.m00465 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 343
Score = 45.6 bits (103), Expect = 3e-05
Identities = 32/82 (39%), Positives = 43/82 (52%), Gaps = 14/82 (17%)
Frame = +2
Query: 329 ICGTQSIHKELENRLSQFHGREDTILYGSCFDANA------GLFESMLTPED-------- 466
ICG + H+ LE+ L+Q +ED ++ + F AN G S+L
Sbjct: 9 ICGYTTYHRLLESSLAQLKKKEDCLVCPTGFAANMAAMVAIGSVASLLAASGKPLKNEKV 68
Query: 467 AVFSDALNHASIIDGSGYARRR 532
A+FSDALNHASIIDG A R+
Sbjct: 69 AIFSDALNHASIIDGVRLAERQ 90
>At1g16960.1 68414.m02059 expressed protein
Length = 114
Score = 32.3 bits (70), Expect = 0.26
Identities = 12/37 (32%), Positives = 24/37 (64%)
Frame = +3
Query: 84 GVAKLRDVLEDRLQEIKRAKTWKHERVLTSPQDTKVR 194
G + R + E + ++I+R KTWKH + ++ + T++R
Sbjct: 2 GCSSSRTIAEGKKEKIRRPKTWKHPQPISRDELTQMR 38
>At1g22930.1 68414.m02866 T-complex protein 11 contains Pfam
PF05794: T-complex protein 11
Length = 1131
Score = 31.9 bits (69), Expect = 0.35
Identities = 16/36 (44%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +3
Query: 42 QVRHL-HEVKRQERAGVAKLRDVLEDRLQEIKRAKT 146
QVRH+ + V Q +K+RD LED+LQ KR ++
Sbjct: 222 QVRHVANSVSNQREIERSKMRDKLEDKLQRAKRYRS 257
>At5g27230.1 68418.m03248 expressed protein ; expression supported
by MPSS
Length = 948
Score = 29.1 bits (62), Expect = 2.5
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +2
Query: 236 QLFRLIDHPEVVEAAREGLKKYGAGLSSVRFIC 334
+LFRL+ E V A E LKK L++++FIC
Sbjct: 332 KLFRLLGLEEKVSGAVETLKKKEEYLATLKFIC 364
>At1g72210.1 68414.m08349 basic helix-loop-helix (bHLH) family
protein (bHLH096) identical to basic-helix-loop-helix
transcription factor [Arabidopsis thaliana] GI:20520637;
contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain; PMID: 12679534
Length = 320
Score = 28.7 bits (61), Expect = 3.3
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 174 PQDTKVRVQGAQGEFLNFCANNYLG 248
PQDTK ++ QG+ F +NNY G
Sbjct: 36 PQDTKNNIKLGQGQGHGFASNNYNG 60
>At5g43175.1 68418.m05270 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 223
Score = 28.3 bits (60), Expect = 4.3
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +2
Query: 488 NHASIIDGSGYARRRNTDPXRDL 556
N+A+++DGS + RN D +DL
Sbjct: 55 NYAAVLDGSNHQTNRNVDSRQDL 77
>At2g37080.1 68415.m04550 myosin heavy chain-related low similarity
to myosin heavy chain [Rana catesbeiana] GI:4249701
Length = 583
Score = 28.3 bits (60), Expect = 4.3
Identities = 16/61 (26%), Positives = 33/61 (54%)
Frame = +3
Query: 42 QVRHLHEVKRQERAGVAKLRDVLEDRLQEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFL 221
Q+R +E + ++G A+ L + L++ K + HER++ ++ K+R+ + E L
Sbjct: 323 QIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLM--DKEAKLRILVDENEIL 380
Query: 222 N 224
N
Sbjct: 381 N 381
>At5g52580.1 68418.m06529 hypothetical protein
Length = 327
Score = 27.9 bits (59), Expect = 5.7
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +2
Query: 476 SDALNHASIIDGSGYARRRNTDPXRDLT 559
SD N S + SG+ ++++ DP RDL+
Sbjct: 187 SDVGNRVSSVSQSGFRKQKSHDPTRDLS 214
>At3g28300.1 68416.m03535 integrin-related protein 14a identical to
integrin-related At14a protein GI:11994573 [Arabidopsis
thaliana]
Length = 385
Score = 27.9 bits (59), Expect = 5.7
Identities = 18/77 (23%), Positives = 35/77 (45%)
Frame = +2
Query: 278 AREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLT 457
++E + KY A L + CG H EL++ S+ + ++ DA GL +
Sbjct: 4 SKENMLKYSAHLRAYNSACGD---HPELKSFDSELQQKTSNLINSFTSDAKTGL---VPL 57
Query: 458 PEDAVFSDALNHASIID 508
P+ A + + H + ++
Sbjct: 58 PQHAAYKEFTKHLAEVN 74
>At3g28290.1 68416.m03533 integrin-related protein 14a identical to
At14a protein GI:11994573 [Arabidopsis thaliana] [Gene
230 (1), 33-40 (1999)], At14a protein [Arabidopsis
thaliana] GI:4589123
Length = 385
Score = 27.9 bits (59), Expect = 5.7
Identities = 18/77 (23%), Positives = 35/77 (45%)
Frame = +2
Query: 278 AREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLT 457
++E + KY A L + CG H EL++ S+ + ++ DA GL +
Sbjct: 4 SKENMLKYSAHLRAYNSACGD---HPELKSFDSELQQKTSNLINSFTSDAKTGL---VPL 57
Query: 458 PEDAVFSDALNHASIID 508
P+ A + + H + ++
Sbjct: 58 PQHAAYKEFTKHLAEVN 74
>At1g50660.1 68414.m05696 expressed protein similar to liver stage
antigen-1 (GI:510184) [Plasmodium falciparum]; similar
to Myosin II heavy chain, non muscle (Swiss-Prot:P08799)
[Dictyostelium discoideum]; similar to liver stage
antigen (GI:9916) [Plasmodium falciparum]; similar to
Kinesin-like protein KLPA (Swiss-Prot:P28739)
[Emericella nidulans]
Length = 725
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/47 (27%), Positives = 28/47 (59%)
Frame = +3
Query: 60 EVKRQERAGVAKLRDVLEDRLQEIKRAKTWKHERVLTSPQDTKVRVQ 200
E++ +R ++ LR+ ++D + ++ A+ W+ ERV D KV ++
Sbjct: 362 EIEALKRESMS-LREEVDDERRMLQMAEVWREERVQMKLIDAKVALE 407
>At1g24340.1 68414.m03070 monooxygenase family protein similar to
polyketide hydroxylases from several bacterial species;
contains Pfam:PF01360 [Monooxygenase]
Length = 707
Score = 27.5 bits (58), Expect = 7.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 330 SAVLKVYTRS*RTACLSSTAAKTQSFTGLVSTPT 431
S++LK Y R LS+T+ Q+F +S P+
Sbjct: 432 SSILKTYETERRPIALSNTSLSVQNFRAAMSVPS 465
>At3g20350.1 68416.m02578 expressed protein
Length = 673
Score = 27.1 bits (57), Expect = 10.0
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +3
Query: 96 LRDVLEDRLQEIKRAKTWKHERVLTSPQDTKVRVQ 200
LR+ ++D + ++ A+ W+ ERV D KV ++
Sbjct: 350 LREEVDDERRMLQMAEVWREERVQMKLIDAKVTLE 384
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,671,924
Number of Sequences: 28952
Number of extensions: 217961
Number of successful extensions: 712
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 710
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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