Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0694.Seq
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid t... 31 0.49
At1g26150.1 68414.m03192 protein kinase family protein similar t... 30 0.85
At5g48360.1 68418.m05975 formin homology 2 domain-containing pro... 30 1.1
At5g08520.1 68418.m01011 myb family transcription factor contain... 30 1.1
At4g00890.1 68417.m00120 proline-rich family protein contains pr... 30 1.1
At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin fa... 30 1.1
At1g13050.1 68414.m01513 expressed protein 30 1.1
At3g19430.1 68416.m02464 late embryogenesis abundant protein-rel... 29 1.5
At5g07760.1 68418.m00888 formin homology 2 domain-containing pro... 29 2.0
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 29 2.0
At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family... 29 2.0
At2g14890.2 68415.m01692 arabinogalactan-protein (AGP9) identica... 29 2.0
At2g14890.1 68415.m01693 arabinogalactan-protein (AGP9) identica... 29 2.0
At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP1... 29 2.0
At5g38560.1 68418.m04662 protein kinase family protein contains ... 28 4.5
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 27 6.0
At5g61090.1 68418.m07665 proline-rich family protein contains pr... 27 7.9
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 27 7.9
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 27 7.9
At4g04990.1 68417.m00728 expressed protein contains Pfam domain ... 27 7.9
At3g11000.1 68416.m01328 expressed protein 27 7.9
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 27 7.9
At2g35350.1 68415.m04334 protein phosphatase 2C family protein /... 27 7.9
>At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to auxin
down regulated GB:X69640 GI:296442 from [Glycine max];
contains Pfam profile PF00234: Protease inhibitor/seed
storage/LTP family
Length = 297
Score = 31.1 bits (67), Expect = 0.49
Identities = 22/67 (32%), Positives = 28/67 (41%), Gaps = 4/67 (5%)
Frame = -2
Query: 486 PVTKALPVSHPQPIRRAPTV--PAAPIPPQARPGQAQPLQAV--LLPRAVQVHRTLPPLR 319
PV P+ P PI R P + P P P PG P+ LLP LPP+
Sbjct: 109 PVVYPPPIVRPPPITRPPIIIPPIQPPPVTTPPGLLPPITTPPGLLPPVTTPPGLLPPVT 168
Query: 318 LVFPGVI 298
PG++
Sbjct: 169 TP-PGLL 174
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 30.3 bits (65), Expect = 0.85
Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = -2
Query: 486 PVTKALPVSHPQPIRRAPTVPAAPIPPQARPGQAQPL-QAVLLPRAVQVHRTLPP 325
P K +P SH P R P+ PA+ IPP R + P + P + H + PP
Sbjct: 168 PPPKLVPPSHSPP-RHLPSPPASEIPPPPRHLPSPPASERPSTPPSDSEHPSPPP 221
>At5g48360.1 68418.m05975 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 782
Score = 29.9 bits (64), Expect = 1.1
Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 7/58 (12%)
Frame = -2
Query: 468 PVSHPQ-PIRRAPTVPAAPIPPQAR------PGQAQPLQAVLLPRAVQVHRTLPPLRL 316
P+S P P+ +P P P+PP P + A++LPR+ + H T P L L
Sbjct: 47 PISPPFFPLESSPPSPPPPLPPTPPTTFAVFPTFPANISALVLPRSSKPHHTSPTLLL 104
>At5g08520.1 68418.m01011 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 298
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/59 (33%), Positives = 24/59 (40%), Gaps = 2/59 (3%)
Frame = -2
Query: 507 GIEGIQDPVTKALPVSHPQPIRRAPTVPAAPIPPQARPGQAQPL--QAVLLPRAVQVHR 337
G I P PV+ P P A V AAP+P PG A + +PR HR
Sbjct: 241 GTPAIGQPAV-GTPVNLPAPPHMAYGVHAAPVPGSVVPGAAMNIGQMPYTMPRTPTAHR 298
>At4g00890.1 68417.m00120 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 431
Score = 29.9 bits (64), Expect = 1.1
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -2
Query: 468 PVSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTL 331
P+ HPQ ++P +P+ P+++ L+ + V+ HR L
Sbjct: 47 PLFHPQSPPEPKSLPLSPLSPKSKEEPESQTMTPLMSKHVKTHRNL 92
>At2g33790.1 68415.m04144 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245, SP|Q03211 Pistil-specific
extensin-like protein precursor (PELP) {Nicotiana
tabacum}; contains Pfam profile PF01190: Pollen proteins
Ole e I family
Length = 239
Score = 29.9 bits (64), Expect = 1.1
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 4/64 (6%)
Frame = -2
Query: 498 GIQDPVTKALPVSHPQPIRRAPTVP--AAPIPPQARPGQAQPLQAVLLPRAVQVHR--TL 331
G DP +LP P P + PT+P API A P P++ LP A + TL
Sbjct: 31 GSSDPF-HSLPQHLPLPPIKLPTLPPAKAPIKLPAYPPAKAPIKLPTLPPAKAPIKLPTL 89
Query: 330 PPLR 319
PP++
Sbjct: 90 PPIK 93
>At1g13050.1 68414.m01513 expressed protein
Length = 317
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -2
Query: 468 PVSHPQPIRRAPTVPAAPIPPQARPGQAQPLQ 373
P S P P+R P P+PP+ P A+PLQ
Sbjct: 68 PSSRPLPLR-----PEEPLPPRHNPNSARPLQ 94
>At3g19430.1 68416.m02464 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 559
Score = 29.5 bits (63), Expect = 1.5
Identities = 18/52 (34%), Positives = 21/52 (40%), Gaps = 2/52 (3%)
Frame = -2
Query: 507 GIEGIQDPVTKALPVSHPQPIRR--APTVPAAPIPPQARPGQAQPLQAVLLP 358
G +G P PVS P P +PT P +P PP P P V P
Sbjct: 67 GDDGGYTPPAPVPPVSPPPPTPSVPSPTPPVSPPPPTPTPSVPSPTPPVSPP 118
>At5g07760.1 68418.m00888 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 853
Score = 29.1 bits (62), Expect = 2.0
Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
Frame = -2
Query: 486 PVTKALPVSHPQP-IRRAPTVPAAPIPPQARPG-QAQPLQAVLLPRAVQVHRTLPPLRLV 313
P+ + P+ P P R +P P PP P A+ L P V+ LPP L+
Sbjct: 47 PMRRRAPLPPPPPPAMRRRVLPRPPPPPPPLPMFDAEVLCCCYPPTRVRREAPLPPPPLI 106
Query: 312 FPG 304
F G
Sbjct: 107 FVG 109
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/48 (31%), Positives = 18/48 (37%)
Frame = -2
Query: 468 PVSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPP 325
P P P+RR +P P PP R P + R V PP
Sbjct: 27 PPPPPPPMRRRAPLPPPPPPPMRRRAPLPPPPPPAMRRRVLPRPPPPP 74
>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 340
Score = 29.1 bits (62), Expect = 2.0
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = -2
Query: 492 QDPVTKALPVSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLP 328
Q P T L + P P + ++P+ P PP + A+P+ + + + RT P
Sbjct: 186 QIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSSSFHAEPIPPTVGTSSTKRRRTTP 240
>At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family
protein identical to proline-rich protein 2 [Arabidopsis
thaliana] gi|7620011|gb|AAF64549
Length = 321
Score = 29.1 bits (62), Expect = 2.0
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
Frame = -2
Query: 456 PQPIRRAPTV-PAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPL 322
P PI + P V P P PP+ +P + P V +T PPL
Sbjct: 219 PVPIYKPPVVIPKKPCPPKIHKPIYKPPVPIYKPPVVIPKKTFPPL 264
>At2g14890.2 68415.m01692 arabinogalactan-protein (AGP9) identical
to gi|10880495|gb|AAG24277
Length = 176
Score = 29.1 bits (62), Expect = 2.0
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 486 PVTKALPVS-HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRA 352
PV+ PV+ P P+ AP PA P PP + P A P A P A
Sbjct: 47 PVSAPPPVTTSPPPVTTAPP-PANPPPPVSSPPPASPPPATPPPVA 91
>At2g14890.1 68415.m01693 arabinogalactan-protein (AGP9) identical
to gi|10880495|gb|AAG24277
Length = 191
Score = 29.1 bits (62), Expect = 2.0
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 486 PVTKALPVS-HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRA 352
PV+ PV+ P P+ AP PA P PP + P A P A P A
Sbjct: 47 PVSAPPPVTTSPPPVTTAPP-PANPPPPVSSPPPASPPPATPPPVA 91
>At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP19)
non-consensus splice site at the intron:exon boundary
(AT:exon)
Length = 247
Score = 29.1 bits (62), Expect = 2.0
Identities = 21/58 (36%), Positives = 24/58 (41%), Gaps = 4/58 (6%)
Frame = -2
Query: 486 PVTKALPVSHPQPIRRAPTVPAAPIPPQAR--PGQAQPLQAVLLPRAVQVHR--TLPP 325
P PVS P P PA+P P A P A P A + P VQ +LPP
Sbjct: 109 PTVSPPPVSPPPAPTSPPPTPASPPPAPASPPPAPASPPPAPVSPPPVQAPSPISLPP 166
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
Frame = -2
Query: 495 IQDPVTKALPVSHPQPIRRAPTVPAAPIP--PQARPGQAQP 379
I P + + P P++ PT P+AP P P P Q+ P
Sbjct: 9 ILSPPSSNSSTTAPPPLQTQPTTPSAPPPVTPPPSPPQSPP 49
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 27.5 bits (58), Expect = 6.0
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = -2
Query: 459 HPQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLPRAVQV--HRTLPP 325
HPQ + P +P +PP + PL LPR +Q+ H +PP
Sbjct: 580 HPQQMLPMPNMPHHQLPPSSH----MPLHPHHLPRPMQMPPHGHMPP 622
>At5g61090.1 68418.m07665 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
contains similarity to vegetative cell wall protein gp1
[Chlamydomonas reinhardtii] gi|12018147|gb|AAG45420;
common family members: At4g18570, At3g25690, At4g04980
[Arabidopsis thaliana]
Length = 344
Score = 27.1 bits (57), Expect = 7.9
Identities = 22/59 (37%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
Frame = -2
Query: 480 TKALPVSHPQ-PIRRAPT-VPAAPIPPQARPGQAQPLQAVLLP---RAVQVHRTLPPLR 319
T P S P PI P VPA P P RP A+P + P R + +TL P R
Sbjct: 100 TPEAPRSVPACPIPEIPRPVPARPTPETPRPVTARPTPEIPRPVPARPISEVQTLVPTR 158
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 27.1 bits (57), Expect = 7.9
Identities = 14/30 (46%), Positives = 16/30 (53%), Gaps = 1/30 (3%)
Frame = -2
Query: 480 TKALPVSHPQPIRRAPTVPA-APIPPQARP 394
T A VSH P +AP PA P PP +P
Sbjct: 169 TSADAVSHVAPSEKAPEKPAPKPSPPAEKP 198
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains Pfam
profile PF00730: HhH-GPD superfamily base excision DNA
repair protein
Length = 1729
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -2
Query: 456 PQPIRRAPTVPAAPIPPQARPGQAQPLQAVLLP 358
P P R+ T P+PP++ P A P+ + LP
Sbjct: 1402 PAPEERSLTSATIPVPPESYPPVAIPMIELPLP 1434
>At4g04990.1 68417.m00728 expressed protein contains Pfam domain
PF05553: Cotton fiber expressed protein
Length = 303
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/41 (31%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = -2
Query: 507 GIEGIQDPVTKALPVSHPQPI--RRAPTVPAAPIPPQARPG 391
G G+ P + P + +P+ RR PTVP P G
Sbjct: 123 GSYGVSSPEVRFFPTAPEKPVGLRRPPTVPVKTFPQDNTSG 163
>At3g11000.1 68416.m01328 expressed protein
Length = 488
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = -2
Query: 516 FWCGIEGIQDPVTKALPVSHPQPIRRAPTVPAAPIPPQARPGQAQPLQAV 367
FW ++ Q K L + P P R PT+ +PP +P A L +
Sbjct: 124 FWFELDRGQ--TNKLLRLFKPSPSVRPPTISRDAVPPPRKPIPASSLAQI 171
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -2
Query: 480 TKALPVSHPQPIRRAPTVPAAPIP-PQARPGQAQP 379
+ +LP+S Q A VPA+PIP P P Q P
Sbjct: 126 SSSLPISGQQRGGMAIGVPASPIPSPSPTPSQHSP 160
>At2g35350.1 68415.m04334 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2c
(GI:3608412) [Mesembryanthemum crystallinum]; contains
Pfam PF00481 : Protein phosphatase 2C domain
Length = 783
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +3
Query: 144 NLKHYKEYSKSCLVVLNTDILTEMVRNIEYVFEAKAYFP 260
+L+HY+ +VL++D L + + N+E V A FP
Sbjct: 683 SLRHYRLTENDQFMVLSSDGLYQYLSNVEVVSLAMEKFP 721
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,786,397
Number of Sequences: 28952
Number of extensions: 222683
Number of successful extensions: 830
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 660
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 803
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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