Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0690.Seq
         (616 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    77   3e-15
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    51   1e-07
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    35   0.008
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    29   0.53 
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo...    28   1.2  
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch...    26   3.8  
SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c...    26   3.8  
SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual     26   3.8  
SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||...    26   3.8  
SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c...    25   8.7  
SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce...    25   8.7  
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ...    25   8.7  

>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 76.6 bits (180), Expect = 3e-15
 Identities = 41/118 (34%), Positives = 60/118 (50%)
 Frame = +1

Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQ 438
           + + I  P G ++H+    S   Y+F+    GKY YCF N       K V+FN+      
Sbjct: 58  VSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNVH---GV 114

Query: 439 TKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
               +E+   +N L   +++L      +KH+QEY   R+RIHR+  ESTN RV  WSI
Sbjct: 115 IYISDEDLDANNPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSI 172



 Score = 34.3 bits (75), Expect = 0.014
 Identities = 11/39 (28%), Positives = 24/39 (61%)
 Frame = +2

Query: 134 VSNAYSYTVTVDAHAEECFYENVEADTKLGLSFEIAEGG 250
           +S  + + +T+  H  ECFYEN+  + ++ ++++   GG
Sbjct: 16  ISVVFGHGITLKPHQRECFYENLRNNDQMSVTYQTNVGG 54


>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 28/123 (22%), Positives = 54/123 (43%), Gaps = 4/123 (3%)
 Frame = +1

Query: 256 DIDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----E 423
           D+D +I  P    +   ++       F+    G+Y +CF N MS+ T K+V   +    E
Sbjct: 59  DVDYMIKAPSQKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENE 118

Query: 424 IGEPQTKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVM 603
           +  P        +   + ++  + E++T    I   Q Y + R+  + S  +ST  R+  
Sbjct: 119 LSLPALTRDEAKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFW 178

Query: 604 WSI 612
           +S+
Sbjct: 179 FSL 181


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 35.1 bits (77), Expect = 0.008
 Identities = 25/113 (22%), Positives = 45/113 (39%), Gaps = 3/113 (2%)
 Frame = +1

Query: 283 DGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGN--- 453
           + ++I+K +  S+    F+    G +  C+ +             L +        N   
Sbjct: 76  NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135

Query: 454 ENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
            +E +   L D +  L T   +I  +Q   + R+   R  +ES N RVV W+I
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTI 188


>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 29.1 bits (62), Expect = 0.53
 Identities = 19/46 (41%), Positives = 24/46 (52%)
 Frame = +1

Query: 460 EADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRV 597
           EAD  +  D I+E       IK    Y+Q R+   R+ NESTN RV
Sbjct: 145 EADIRRARDFIEE-------IKGKIYYLQAREARFRNTNESTNERV 183


>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 550

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +2

Query: 284 MVTSFIKQRGSHLVFIHFLLQLQGSILIASVIKCHP 391
           MVT  +K  G   + +HF+  + G+ L  ++ KC P
Sbjct: 171 MVTEALKPYGQENLELHFVSNIDGTHLAEALKKCDP 206


>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1318

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -2

Query: 147  YALLTRFNKVTTSHCDILKYFILLNV 70
            YA+L   N +T++ CD   Y +L+ V
Sbjct: 1026 YAILNILNLLTSNECDEFDYSVLMKV 1051


>SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1316

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -2

Query: 510 ISSKFFDHVLKFIMVSFVFITRFSLWFSDF 421
           I SK    VL+ + +S V +TRFSL+   F
Sbjct: 12  IVSKVIFFVLRSVTLSLVSVTRFSLFLLSF 41


>SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 554

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 4/32 (12%)
 Frame = -3

Query: 263 SISRNLLLLFQTII----PIWYRLLHFRKSTL 180
           S+ R  LL F  II    P+W++  H+ +S+L
Sbjct: 70  SLKRYALLSFYVIILLAIPVWWKTTHYERSSL 101


>SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 624

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 17/37 (45%), Positives = 22/37 (59%)
 Frame = -2

Query: 246 PSAISNDNPNLVSASTFS*KHSSACASTVTV*LYALL 136
           PSA+SN  P ++SAST S   SS  A  +   L+A L
Sbjct: 582 PSAVSNYAPTVLSASTTS-GTSSVRAKPIVFYLFASL 617


>SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 680

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 9/26 (34%), Positives = 15/26 (57%)
 Frame = +1

Query: 241 RRRFLDIDVIILGPDGNVIHKAERES 318
           + RF D  V  +  DGN++H  + E+
Sbjct: 70  KARFTDFAVFEVDTDGNIVHLTDMEA 95


>SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1112

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 4/54 (7%)
 Frame = +1

Query: 358 YTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNEN----EADHNKLEDMIKELAT 507
           Y     ++  SM P+ ++   ++ E +  S +EN    EAD+N  +D+I +LAT
Sbjct: 352 YDVPLPSRSHSMNPRELVA-AKLKEIKENSFSENQQSDEADYNVTDDLILQLAT 404


>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 444

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/54 (20%), Positives = 26/54 (48%)
 Frame = +1

Query: 244 RRFLDIDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKV 405
           RR+++  ++ +   G++I   +  S G+   +       T C+   +  +TPK+
Sbjct: 147 RRWINSTLVAIAKTGDLIPLVDGGSEGLKGQARVIIPTITSCYECSLDMLTPKI 200


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,556,274
Number of Sequences: 5004
Number of extensions: 52442
Number of successful extensions: 136
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)