Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0690.Seq
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain... 69 2e-12
At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar ... 49 2e-06
At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 46 2e-05
At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar ... 46 2e-05
At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar ... 45 3e-05
At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar ... 42 4e-04
At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar ... 42 4e-04
At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar ... 41 7e-04
At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar ... 40 0.002
At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar ... 40 0.002
At2g03040.1 68415.m00257 transmembrane protein-related low simil... 31 0.80
At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 30 1.1
At1g75850.1 68414.m08810 vacuolar protein sorting-associated pro... 29 1.9
At3g02430.1 68416.m00230 hypothetical protein contains Pfam prof... 29 3.2
At2g30920.1 68415.m03769 hexaprenyldihydroxybenzoate methyltrans... 28 5.7
At1g06660.1 68414.m00706 expressed protein 28 5.7
At3g49320.1 68416.m05392 expressed protein contains Pfam profile... 27 7.5
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 27 7.5
At4g10360.1 68417.m01701 expressed protein 27 9.9
At2g41890.1 68415.m05181 curculin-like (mannose-binding) lectin ... 27 9.9
>At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains
weak similarity to transmembrane protein (GI:1212965)
[Homo sapiens]
Length = 214
Score = 69.3 bits (162), Expect = 2e-12
Identities = 34/118 (28%), Positives = 59/118 (50%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQ 438
+D + P GN++ + S + F AP +G Y +CF N S TP+ V F + +G
Sbjct: 69 LDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYS--TPETVSFYIHVGHIP 126
Query: 439 TKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
+ + + + I EL ++ +Q+Y++ RD HR NEST RV+ +++
Sbjct: 127 NEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTV 184
>At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar to
SP|Q15363 Cop-coated vesicle membrane protein p24
precursor (p24A) {Homo sapiens}; contains Pfam profile
PF01105: emp24/gp25L/p24 family
Length = 208
Score = 49.2 bits (112), Expect = 2e-06
Identities = 28/119 (23%), Positives = 56/119 (47%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQ 438
+D++I GP G IH + S + F G Y +CF+NK S + + F++++G
Sbjct: 63 VDLVIHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNK--SPYHETIDFDVQLGHFA 120
Query: 439 TKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSIF 615
+ + L + I +L I+ +Q +++ + +NE+ + R V ++F
Sbjct: 121 YYDQHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALF 179
>At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 46.4 bits (105), Expect = 2e-05
Identities = 32/124 (25%), Positives = 54/124 (43%), Gaps = 3/124 (2%)
Frame = +1
Query: 250 FLDIDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIG 429
F + V + P G V+H E ++G + F+ +G Y CF S K N++
Sbjct: 62 FPTVSVKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINIDWK 121
Query: 430 EPQTKSGNENEADHNKLEDM---IKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVV 600
++ A K+E + K+L I + Y++ R+ R ++E TN RV
Sbjct: 122 TGIAAKDWDSIARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVA 181
Query: 601 MWSI 612
+SI
Sbjct: 182 WYSI 185
>At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105:
emp24/gp25L/p24 family
Length = 214
Score = 46.0 bits (104), Expect = 2e-05
Identities = 38/124 (30%), Positives = 60/124 (48%), Gaps = 6/124 (4%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKV---VMFNLEIG 429
I V + GN H AE+ SG + FSA AG Y CF+ P+V + F + G
Sbjct: 68 ISVKVTSNSGNNYHHAEQVDSGQFAFSAVEAGDYMACFT--AVDHKPEVSLSIDFEWKTG 125
Query: 430 EPQTKSGNENEADHNKLEDM---IKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVV 600
Q+KS N A +++E M +K L +I + Y++ R+ + +N STN ++
Sbjct: 126 -VQSKSW-ANVAKKSQVEVMEFEVKSLLDTVNSIHEEMYYLRDREEEMQDLNRSTNTKMA 183
Query: 601 MWSI 612
S+
Sbjct: 184 WLSV 187
>At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 217
Score = 45.2 bits (102), Expect = 3e-05
Identities = 33/121 (27%), Positives = 54/121 (44%), Gaps = 3/121 (2%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQ 438
I V + P GN +H E + G + F+ +G Y CF S K V N++
Sbjct: 70 ISVKVTSPYGNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNVSINIDWRTGI 129
Query: 439 TKSGNENEADHNKLEDM---IKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWS 609
+ A K+E + I++L I + Y++ R+ R+++E TN RV +S
Sbjct: 130 AAKDWASIAKKEKIEGVELEIRKLEGAVEAIHENILYLRNREADMRTMSEKTNSRVAWYS 189
Query: 610 I 612
I
Sbjct: 190 I 190
>At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 170
Score = 41.5 bits (93), Expect = 4e-04
Identities = 28/121 (23%), Positives = 57/121 (47%), Gaps = 2/121 (1%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSN-KMSSMTPKVVMFNLEIGEP 435
I V ++ P G +H+A++ +G ++F+A G Y C + T + F+ + G
Sbjct: 24 IIVKVMPPQGKNLHEADKVEAGQFSFTAYENGSYVACITAIDYKPETTLTIDFDWKTGVH 83
Query: 436 QTKSGN-ENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
+ N ++ + +E +K L +I + Y++ R+ + +N STN ++ W
Sbjct: 84 SKEWTNVAKKSQVDMMEYQVKTLMDTVISIHEEMYYLREREEEMQELNRSTNSKMA-WLS 142
Query: 613 F 615
F
Sbjct: 143 F 143
>At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile PF01105: emp24/gp25L/p24
family
Length = 216
Score = 41.5 bits (93), Expect = 4e-04
Identities = 28/117 (23%), Positives = 52/117 (44%), Gaps = 3/117 (2%)
Frame = +1
Query: 271 ILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSG 450
+ P GN +H E + G + F+ AG Y CF S + ++
Sbjct: 73 VTSPYGNNLHHQENVTHGQFAFTTQEAGNYLACFWIDSSHHLANPITLGVDWKMGIAAKD 132
Query: 451 NENEADHNKLEDM---IKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
++ A K+E + ++ L +I+ + Y++ R+ R ++E+TN RV +SI
Sbjct: 133 WDSVAKKEKIEGVELQLRRLEGLVLSIRENLNYIKDREAEMREVSETTNSRVAWFSI 189
>At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105
emp24/gp25L/p24 family
Length = 204
Score = 40.7 bits (91), Expect = 7e-04
Identities = 33/118 (27%), Positives = 49/118 (41%), Gaps = 2/118 (1%)
Frame = +1
Query: 265 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPK-VVMFNLEIG-EPQ 438
V + P G H AE SG + F+A G Y CF T K V F + G E +
Sbjct: 60 VTVSSPKGKSHHHAENVESGKFVFTAEETGDYMTCFVAPGYRPTAKFAVDFEWKSGVEAK 119
Query: 439 TKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMWSI 612
+ LE +++L TI + + R+R + +N STN R+ S+
Sbjct: 120 DWTTIAKRGQITMLEVEVRKLLDVTETIHEEMFQLIEREREMQELNRSTNSRMAALSL 177
>At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 214
Score = 39.5 bits (88), Expect = 0.002
Identities = 29/123 (23%), Positives = 57/123 (46%), Gaps = 4/123 (3%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVM---FNLEIG 429
I + + GN H AE SG + F+A +G Y C++ P+V + F+ G
Sbjct: 68 ISIRVTSSYGNTYHHAEDVESGQFAFTAVESGDYMACYT--AVDHKPEVTLSIDFDWRTG 125
Query: 430 -EPQTKSGNENEADHNKLEDMIKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMW 606
+ ++ S ++ +E +K L +I + Y++ R+ +++N +TN ++ W
Sbjct: 126 VQSKSWSSVAKKSQVEVMEFDVKRLIETVNSIHEEMFYLREREEEMQNLNRATNSKMA-W 184
Query: 607 SIF 615
F
Sbjct: 185 LSF 187
>At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 39.5 bits (88), Expect = 0.002
Identities = 32/123 (26%), Positives = 57/123 (46%), Gaps = 4/123 (3%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSN-KMSSMTPKVVMFNLEIGEP 435
+ V + P G H+++ SG ++F A G Y CFS T ++ F+ G
Sbjct: 66 VTVRVTSPQGTAYHESDGVESGQFSFVAVETGDYISCFSAVDHKPETTLIIDFDWRTG-I 124
Query: 436 QTKSGNENEADHNKLEDM---IKELATN*RTIKHDQEYMQVRDRIHRSINESTNXRVVMW 606
TK + N A +++E M +K+L I + Y++ R+ ++N +TN ++ W
Sbjct: 125 HTKDWS-NVAKKSQVETMEFEVKKLFETVNGIHDEMFYLRDREEEMHNLNIATNSKMA-W 182
Query: 607 SIF 615
F
Sbjct: 183 LSF 185
>At2g03040.1 68415.m00257 transmembrane protein-related low
similarity to SP|Q28735|TM21_RABIT Transmembrane protein
Tmp21 precursor (21 kDa Transmembrane trafficking
protein) (Integral membrane protein p23) {Oryctolagus
cuniculus}
Length = 166
Score = 30.7 bits (66), Expect = 0.80
Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 2/100 (2%)
Frame = +1
Query: 259 IDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSN-KMSSMTPKVVMFNLEIGEP 435
I V ++ P G +H+A+ +G ++F+A G Y C + T + F+ + G
Sbjct: 67 IIVKVMPPQGKNLHEADNVEAGQFSFTAYENGSYVACITAIDYKPETTLTIDFDWKTGVH 126
Query: 436 QTKSGN-ENEADHNKLEDMIKELATN*RTIKHDQEYMQVR 552
+ N ++ + +E +K L +I + Y++ R
Sbjct: 127 SKEWTNVAKKSQVDMMEYQVKTLMDTVISIHEEMYYLRER 166
>At5g05210.1 68418.m00555 nucleolar matrix protein-related contains
Pfam domain, PF04935: Surfeit locus protein 6
Length = 386
Score = 30.3 bits (65), Expect = 1.1
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = +1
Query: 451 NENEADHNKLEDMIKELATN*RTIKHDQE--YMQVRDRIHRSINESTNXR 594
++ E D+N+L D + T+ K D+ Y ++R+R+HR I+E R
Sbjct: 135 SKKETDNNRLNDDDSKEETDNNRQKDDRSVTYEELRERLHRKIDELKGGR 184
>At1g75850.1 68414.m08810 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 838
Score = 29.5 bits (63), Expect = 1.9
Identities = 17/57 (29%), Positives = 24/57 (42%)
Frame = +1
Query: 304 AERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADHN 474
A R SSG T KY Y F +TP + +E+ + +S N N H+
Sbjct: 752 ATRGSSGPVTLFVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHS 808
>At3g02430.1 68416.m00230 hypothetical protein contains Pfam profile
PF05078: Protein of unknown function (DUF679)
Length = 219
Score = 28.7 bits (61), Expect = 3.2
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +2
Query: 86 KY-LRISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADTK 217
KY +R W+ TL LV A + D + +CFY + EA+TK
Sbjct: 139 KYRMRFVDWIHATLSVLVFGAVALR---DKYITDCFYPSPEAETK 180
>At2g30920.1 68415.m03769 hexaprenyldihydroxybenzoate
methyltransferase identical to
hexaprenyldihydroxybenzoate methyltransferase SP:O49354
from [Arabidopsis thaliana]
Length = 322
Score = 27.9 bits (59), Expect = 5.7
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -3
Query: 314 SLSAL*MTLPSGPSIITSISRNLLLLFQTIIPIWYRLLHFRKST 183
SLSAL T+P+G +++++I+R + TI+ Y L K T
Sbjct: 220 SLSAL--TIPNGATVLSTINRTMRAYASTIVGAEYILRWLPKGT 261
>At1g06660.1 68414.m00706 expressed protein
Length = 481
Score = 27.9 bits (59), Expect = 5.7
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 95 RISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADT 214
RI V ++ N++ NA Y V D+H + E +EA+T
Sbjct: 290 RIRSQFVHSVSNIMENASLYKVYKDSHEGLDYEEQIEAET 329
>At3g49320.1 68416.m05392 expressed protein contains Pfam profile
PF03690: Uncharacterised protein family (UPF0160)
Length = 354
Score = 27.5 bits (58), Expect = 7.5
Identities = 19/80 (23%), Positives = 34/80 (42%)
Frame = +1
Query: 244 RRFLDIDVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLE 423
+R D+ + L N I + +GI+ + +Y +S+ ++ NL+
Sbjct: 134 QRHPDVFRLFLAVYKNFIEAVDALDNGIHQYDTDQPPRYV-----NNTSLGHRIGRLNLD 188
Query: 424 IGEPQTKSGNENEADHNKLE 483
EP S E+EA H +E
Sbjct: 189 WIEPDQSSAKEDEAFHRAME 208
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 27.5 bits (58), Expect = 7.5
Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +2
Query: 284 MVTSFIKQRGSHLVFIHFLLQLQGSILIAS-VIKCHP*HPRW 406
++ + G+H+VFIH QL G L+ + V P RW
Sbjct: 369 LIRKLADESGNHVVFIHMDDQLDGKTLVGTYVCTDQPGEFRW 410
>At4g10360.1 68417.m01701 expressed protein
Length = 266
Score = 27.1 bits (57), Expect = 9.9
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -1
Query: 604 TSPHVNLYFHLLNDGYDHAPAYTLDRV 524
T+P VNL ++L N G + AYTL+ +
Sbjct: 164 TTPFVNLRWYLDNSGQKGSKAYTLNGI 190
>At2g41890.1 68415.m05181 curculin-like (mannose-binding) lectin
family protein / PAN domain-containing protein contains
Pfam profiles: PF01453 lectin (probable mannose
binding), PF00024 PAN domain
Length = 764
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = +1
Query: 217 IGIIV*NSRRRFLDIDVIILGPDGNVIHKAERESSGIYTFSAPT 348
+G+ V NS+ + +L DGN++ +RE +F PT
Sbjct: 111 LGVPVWNSKTNRFSVSSALLRDDGNLVLLKDREEIVWQSFGTPT 154
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,901,926
Number of Sequences: 28952
Number of extensions: 254720
Number of successful extensions: 626
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 609
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 624
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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