Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0682.Seq
(615 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino... 76 2e-14
At4g38220.2 68417.m05395 aminoacylase, putative / N-acyl-L-amino... 73 2e-13
At4g38220.1 68417.m05394 aminoacylase, putative / N-acyl-L-amino... 73 2e-13
At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino... 69 3e-12
At5g14850.2 68418.m01742 mannosyltransferase, putative similar t... 31 0.46
At5g14850.1 68418.m01741 mannosyltransferase, putative similar t... 31 0.46
At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase, puta... 31 0.80
At4g21540.1 68417.m03112 diacylglycerol kinase family protein co... 29 3.2
At5g09930.1 68418.m01148 ABC transporter family protein 28 5.7
At5g08070.1 68418.m00941 TCP family transcription factor, putati... 28 5.7
At3g29630.1 68416.m03726 glycosyltransferase family protein cont... 28 5.7
At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si... 27 7.5
>At1g44820.1 68414.m05134 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 438
Score = 76.2 bits (179), Expect = 2e-14
Identities = 32/79 (40%), Positives = 49/79 (62%)
Frame = +1
Query: 19 ASTYQNNPSIANFVEYLKIPSVQPNIDYSDCVKFLTRQANEIGLAVKIYEVVPTKPIVVL 198
+S+ + + I F +YL+ + PN +Y+ + FL QA IGL K E + KPI+++
Sbjct: 21 SSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKTIEFISGKPILLI 80
Query: 199 TWTGKEPNLPAILLNSHME 255
TW G PNLP+IL NSH++
Sbjct: 81 TWLGSNPNLPSILFNSHLD 99
Score = 66.5 bits (155), Expect = 1e-11
Identities = 27/43 (62%), Positives = 32/43 (74%)
Frame = +3
Query: 258 VPVFENSWTYPPFSGHIDNDGKIFARGSQDMKCVGIQYLEAIR 386
VP WTYPPFS H DG I+ARG+QD KC+G+QYLE+IR
Sbjct: 101 VPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLESIR 143
Score = 53.6 bits (123), Expect = 1e-07
Identities = 27/56 (48%), Positives = 37/56 (66%)
Frame = +2
Query: 377 SDKEIESRQVALKRTLHISFVPDEEIGGHDGMEKFVKMNEFKALILVSLWMKGMAS 544
S + ++SR + RT+HIS+VP+EEIGG DGM KF +EFK L L +G A+
Sbjct: 141 SIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGFAMDEGQAN 196
>At4g38220.2 68417.m05395 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 433
Score = 72.5 bits (170), Expect = 2e-13
Identities = 34/84 (40%), Positives = 49/84 (58%)
Frame = +1
Query: 46 IANFVEYLKIPSVQPNIDYSDCVKFLTRQANEIGLAVKIYEVVPTKPIVVLTWTGKEPNL 225
++ F EYL+I +VQPN +Y V F+ QA + L + E V KP+++L W G +P L
Sbjct: 25 VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84
Query: 226 PAILLNSHMELCRFSKTVGHIHRL 297
PA LLNSH ++ F + H L
Sbjct: 85 PAFLLNSHTDVVPFEDSKWTHHPL 108
Score = 67.7 bits (158), Expect = 6e-12
Identities = 28/44 (63%), Positives = 35/44 (79%)
Frame = +3
Query: 255 VVPVFENSWTYPPFSGHIDNDGKIFARGSQDMKCVGIQYLEAIR 386
VVP ++ WT+ P H+D+ G I+ARGSQDMKCVG+QYLEAIR
Sbjct: 95 VVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIR 138
Score = 51.6 bits (118), Expect = 4e-07
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = +2
Query: 416 RTLHISFVPDEEIGGHDGMEKFVKMNEFKALILVSLWMKGMASS*RGFHWF 568
R++++SFVPDEEIGGHDG EKF + FK+L + + +G+ S + F
Sbjct: 149 RSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVF 199
>At4g38220.1 68417.m05394 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 430
Score = 72.5 bits (170), Expect = 2e-13
Identities = 34/84 (40%), Positives = 49/84 (58%)
Frame = +1
Query: 46 IANFVEYLKIPSVQPNIDYSDCVKFLTRQANEIGLAVKIYEVVPTKPIVVLTWTGKEPNL 225
++ F EYL+I +VQPN +Y V F+ QA + L + E V KP+++L W G +P L
Sbjct: 25 VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84
Query: 226 PAILLNSHMELCRFSKTVGHIHRL 297
PA LLNSH ++ F + H L
Sbjct: 85 PAFLLNSHTDVVPFEDSKWTHHPL 108
Score = 67.7 bits (158), Expect = 6e-12
Identities = 28/44 (63%), Positives = 35/44 (79%)
Frame = +3
Query: 255 VVPVFENSWTYPPFSGHIDNDGKIFARGSQDMKCVGIQYLEAIR 386
VVP ++ WT+ P H+D+ G I+ARGSQDMKCVG+QYLEAIR
Sbjct: 95 VVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIR 138
Score = 51.6 bits (118), Expect = 4e-07
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = +2
Query: 416 RTLHISFVPDEEIGGHDGMEKFVKMNEFKALILVSLWMKGMASS*RGFHWF 568
R++++SFVPDEEIGGHDG EKF + FK+L + + +G+ S + F
Sbjct: 149 RSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVF 199
>At1g44180.1 68414.m05103 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1) [Homo sapiens] SWISS-PROT:Q03154
Length = 435
Score = 68.5 bits (160), Expect = 3e-12
Identities = 30/75 (40%), Positives = 45/75 (60%)
Frame = +1
Query: 31 QNNPSIANFVEYLKIPSVQPNIDYSDCVKFLTRQANEIGLAVKIYEVVPTKPIVVLTWTG 210
+ + I F +YL+ + PN +Y+ + FL QA IGL + E VP KP+++LTW G
Sbjct: 27 EEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTSRTIEFVPGKPVLLLTWLG 86
Query: 211 KEPNLPAILLNSHME 255
NL +IL NSH++
Sbjct: 87 SNLNLSSILFNSHLD 101
Score = 63.3 bits (147), Expect = 1e-10
Identities = 26/43 (60%), Positives = 31/43 (72%)
Frame = +3
Query: 258 VPVFENSWTYPPFSGHIDNDGKIFARGSQDMKCVGIQYLEAIR 386
VP W +PPFS H DG I+ARG+QD KC+G+QYLEAIR
Sbjct: 103 VPAESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIR 145
Score = 53.2 bits (122), Expect = 1e-07
Identities = 26/54 (48%), Positives = 36/54 (66%)
Frame = +2
Query: 383 KEIESRQVALKRTLHISFVPDEEIGGHDGMEKFVKMNEFKALILVSLWMKGMAS 544
+ ++SR + RT+HIS+VP+EEIGG GM KF +EFK L L + +G AS
Sbjct: 145 RNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKDLNLGFVMDEGQAS 198
>At5g14850.2 68418.m01742 mannosyltransferase, putative similar to
PIGB from Homo sapiens [gi:1552169], Mus musculus
[gi:7634741]
Length = 529
Score = 31.5 bits (68), Expect = 0.46
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = -2
Query: 221 FGSFPVHVKTTIGFVGTTSYIFTAKPISFACLVKNLTQSL*SMFGWTLGIF 69
+G+ P H T GF+ +FT P S A ++K+ Q L ++ W L I+
Sbjct: 285 YGTHPWHWYFTQGFL---VMLFTFTPFSIAGIIKSKNQKLSALILWVLAIY 332
>At5g14850.1 68418.m01741 mannosyltransferase, putative similar to
PIGB from Homo sapiens [gi:1552169], Mus musculus
[gi:7634741]
Length = 548
Score = 31.5 bits (68), Expect = 0.46
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = -2
Query: 221 FGSFPVHVKTTIGFVGTTSYIFTAKPISFACLVKNLTQSL*SMFGWTLGIF 69
+G+ P H T GF+ +FT P S A ++K+ Q L ++ W L I+
Sbjct: 285 YGTHPWHWYFTQGFL---VMLFTFTPFSIAGIIKSKNQKLSALILWVLAIY 332
>At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase,
putative similar to N-carbamoyl-L-amino acid hydrolase
[Bacillus stearothermophilus] SWISS-PROT:Q53389
Length = 476
Score = 30.7 bits (66), Expect = 0.80
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +1
Query: 202 WTGKEPNLPAILLNSHMELCRFS 270
W G EPNLPA+ SH++ +S
Sbjct: 123 WDGLEPNLPAVATGSHIDAIPYS 145
>At4g21540.1 68417.m03112 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 1240
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +3
Query: 288 PPFSGHIDNDGKIFARGSQDMKC 356
P G ID+DG++ ARG + KC
Sbjct: 719 PDKEGIIDSDGEVLARGKRTYKC 741
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +3
Query: 288 PPFSGHIDNDGKIFARGSQDMKC 356
P G ID+DG++ ARG + KC
Sbjct: 1192 PDKEGIIDSDGEVLARGRKSYKC 1214
>At5g09930.1 68418.m01148 ABC transporter family protein
Length = 678
Score = 27.9 bits (59), Expect = 5.7
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +3
Query: 117 ILNETSERNWFSRKNIRGRADETDRRFDMDRKRTK 221
+++ + N+F KN+ RA E +R +++ K K
Sbjct: 604 LMDYAGDYNYFLEKNVEARARELEREAELEEKAPK 638
>At5g08070.1 68418.m00941 TCP family transcription factor, putative
similar to TFPD (GI:6681577) [Arabidopsis thaliana] ;
putative basic helix-loop-helix DNA binding protein TCP2
- Arabidopsis thaliana, EMBL:AF072691
Length = 242
Score = 27.9 bits (59), Expect = 5.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -3
Query: 340 EPLAKIFPSLSMCPLNGGYVQLFSKTGTTP 251
E ++ +FP+ L GG +QLFS T + P
Sbjct: 209 ETMSSLFPTRYPSFLGGGQLQLFSSTSSQP 238
>At3g29630.1 68416.m03726 glycosyltransferase family protein
contains Pfam profile: PF00201 UDP-glucoronosyl and
UDP-glucosyl transferase
Length = 448
Score = 27.9 bits (59), Expect = 5.7
Identities = 10/36 (27%), Positives = 24/36 (66%)
Frame = +1
Query: 67 LKIPSVQPNIDYSDCVKFLTRQANEIGLAVKIYEVV 174
+KI S++P++ + D V ++ + A E+G+ Y+++
Sbjct: 101 VKIRSLKPDLIFFDFVDWIPQMAKELGIKSVSYQII 136
>At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein
similar to acetylornithine deacetylase
(Acetylornithinase, AO; N-acetylornithinase, NAO)
[Dictyostelium discoideum] SWISS-PROT:P54638
Length = 440
Score = 27.5 bits (58), Expect = 7.5
Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 3/48 (6%)
Frame = +3
Query: 228 GNIIEFA---YGVVPVFENSWTYPPFSGHIDNDGKIFARGSQDMKCVG 362
G I+ F VV + W + PFS ID D K+ RG+ D C+G
Sbjct: 92 GKILSFVGMHMDVVTANPDDWEFDPFSLSIDGD-KLRGRGTTD--CLG 136
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,530,429
Number of Sequences: 28952
Number of extensions: 290607
Number of successful extensions: 776
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 750
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -