Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0671.Seq
(449 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P48735 Cluster: Isocitrate dehydrogenase [NADP], mitoch... 137 1e-31
UniRef50_Q8LPJ5 Cluster: Isocitrate dehydrogenase-like protein; ... 133 1e-30
UniRef50_Q0CXI1 Cluster: Isocitrate dehydrogenase, mitochondrial... 131 8e-30
UniRef50_Q9SRZ6 Cluster: F12P19.10 protein; n=26; cellular organ... 128 6e-29
UniRef50_A2XVE4 Cluster: Putative uncharacterized protein; n=2; ... 117 1e-25
UniRef50_A5ZVX5 Cluster: Putative uncharacterized protein; n=1; ... 106 3e-22
UniRef50_A2WMU2 Cluster: Putative uncharacterized protein; n=5; ... 100 1e-20
UniRef50_A5N5L9 Cluster: Idh; n=2; Bacteria|Rep: Idh - Clostridi... 99 5e-20
UniRef50_Q4VCC2 Cluster: Isocitrate dehydrogenase; n=4; Eukaryot... 84 2e-15
UniRef50_A3JDN6 Cluster: Isocitrate dehydrogenase; n=5; Gammapro... 37 0.18
UniRef50_Q3ISP1 Cluster: DNA polymerase; n=1; Natronomonas phara... 33 3.8
UniRef50_A7AG60 Cluster: Putative uncharacterized protein; n=1; ... 32 5.0
>UniRef50_P48735 Cluster: Isocitrate dehydrogenase [NADP],
mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate
decarboxylase) (IDH) (NADP(+)-specific ICDH); n=493;
cellular organisms|Rep: Isocitrate dehydrogenase [NADP],
mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate
decarboxylase) (IDH) (NADP(+)-specific ICDH) - Homo
sapiens (Human)
Length = 452
Score = 137 bits (331), Expect = 1e-31
Identities = 65/126 (51%), Positives = 83/126 (65%), Gaps = 4/126 (3%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERR-VLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181
DFV + G ++V+T +DG+ + +Y+ GV MGMYNTDESI FAHS FQ A+QK
Sbjct: 183 DFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQK 242
Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352
KWPLY+ST+ + F+ FQE+F YKT FD+ KIWYEHRLIDDMV Q +K
Sbjct: 243 KWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSG 302
Query: 353 GSMWXC 370
G +W C
Sbjct: 303 GFVWAC 308
>UniRef50_Q8LPJ5 Cluster: Isocitrate dehydrogenase-like protein;
n=6; core eudicotyledons|Rep: Isocitrate
dehydrogenase-like protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 485
Score = 133 bits (322), Expect = 1e-30
Identities = 64/126 (50%), Positives = 85/126 (67%), Gaps = 4/126 (3%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRV-LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181
D V+ PGK+++V+ DG + +YD K PGVA+ MYN DESIR+FA SS +AL K
Sbjct: 215 DTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESIRAFAESSMAMALTK 274
Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352
KWPLYLST+ + F+ FQEV+++++K KF+E IWYEHRLIDDMV A+K
Sbjct: 275 KWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEHSIWYEHRLIDDMVAYAVKSEG 334
Query: 353 GSMWXC 370
G +W C
Sbjct: 335 GYVWAC 340
>UniRef50_Q0CXI1 Cluster: Isocitrate dehydrogenase, mitochondrial;
n=2; Eurotiomycetidae|Rep: Isocitrate dehydrogenase,
mitochondrial - Aspergillus terreus (strain NIH 2624)
Length = 466
Score = 131 bits (316), Expect = 8e-30
Identities = 64/117 (54%), Positives = 83/117 (70%), Gaps = 1/117 (0%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPG-VAMGMYNTDESIRSFAHSSFQVALQK 181
D V+P PGK+ELVYT +G E +YD ++ G VAM MYNTD+SIR FAHSSF++AL K
Sbjct: 199 DRVIPGPGKLELVYTPANGQPESVQVYDFQSGGGVAMSMYNTDDSIRGFAHSSFKMALLK 258
Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK 352
PLY+ST+ + F+ FQE+++++YK +FD IWYEHRLIDDMV Q IK
Sbjct: 259 GLPLYMSTKNTILKRYDGRFKDIFQEIYEAEYKKEFDAKGIWYEHRLIDDMVAQMIK 315
>UniRef50_Q9SRZ6 Cluster: F12P19.10 protein; n=26; cellular
organisms|Rep: F12P19.10 protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 410
Score = 128 bits (309), Expect = 6e-29
Identities = 62/125 (49%), Positives = 81/125 (64%), Gaps = 3/125 (2%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184
D V+ PGK+ + + +DG TE V GVAM MYNTDESIR+FA +S A +KK
Sbjct: 145 DAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIRAFADASMNTAYEKK 204
Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---G 355
WPLYLST+ + F+ FQEV+++ +K+K+D A IWYEHRLIDDMV A+K G
Sbjct: 205 WPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDAAGIWYEHRLIDDMVAYALKSEGG 264
Query: 356 SMWXC 370
+W C
Sbjct: 265 YVWAC 269
Score = 32.3 bits (70), Expect = 5.0
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +1
Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447
C NYDG V F+A G+ SLG+M
Sbjct: 269 CKNYDGDVQSDFLAQGFGSLGLM 291
>UniRef50_A2XVE4 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 371
Score = 117 bits (281), Expect = 1e-25
Identities = 55/100 (55%), Positives = 69/100 (69%), Gaps = 3/100 (3%)
Frame = +2
Query: 80 LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQE 259
+Y+ K PGVA+ MYN DESIR+FA SS +AL KKWPLYLST+ + F+ FQE
Sbjct: 44 VYNFKGPGVALSMYNVDESIRAFAESSMAMALSKKWPLYLSTKNTILKKYDGRFKDIFQE 103
Query: 260 VFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---GSMWXC 370
V++ +K KF+E IWYEHRLIDDMV A+K G +W C
Sbjct: 104 VYEEKWKEKFEENSIWYEHRLIDDMVAYAVKSEGGYVWAC 143
Score = 58.8 bits (136), Expect = 5e-08
Identities = 28/58 (48%), Positives = 38/58 (65%)
Frame = +2
Query: 131 ESIRSFAHSSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKI 304
+SIR+FA SS +AL KKWPLYLST+ + F+ FQEV++ +K KF+E I
Sbjct: 157 QSIRAFAESSMAMALSKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKEKFEENSI 214
>UniRef50_A5ZVX5 Cluster: Putative uncharacterized protein; n=1;
Ruminococcus obeum ATCC 29174|Rep: Putative
uncharacterized protein - Ruminococcus obeum ATCC 29174
Length = 417
Score = 106 bits (254), Expect = 3e-22
Identities = 52/125 (41%), Positives = 77/125 (61%), Gaps = 3/125 (2%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184
+ + KPGKVELVYT+ DG +R ++ + K PGVAMGM+N SI SFA S F AL K
Sbjct: 158 EMYIDKPGKVELVYTSEDGEEKRSLVQEFKAPGVAMGMHNMTASIESFARSCFNYALDTK 217
Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---G 355
++ ++ +T F+ FQ VF +++K +F+EA + Y + LIDD+V + +K G
Sbjct: 218 QDVWFGAKDTISKTYDAKFKEVFQTVFDTEFKDRFEEAGLTYFYSLIDDIVARVMKAEGG 277
Query: 356 SMWXC 370
+W C
Sbjct: 278 FIWAC 282
>UniRef50_A2WMU2 Cluster: Putative uncharacterized protein; n=5;
Eukaryota|Rep: Putative uncharacterized protein - Oryza
sativa subsp. indica (Rice)
Length = 475
Score = 100 bits (240), Expect = 1e-20
Identities = 59/143 (41%), Positives = 80/143 (55%), Gaps = 21/143 (14%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184
D V+ PGK++LV+ R+ E V GVA+ MYNTDESI +FA +S +A QK+
Sbjct: 188 DTVIKGPGKLKLVFDGREEQIELDVFNFTGAGGVALSMYNTDESIWAFAEASMNMAYQKR 247
Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIW------------------Y 310
WPLYLST+ + F+ FQE +++ ++ KFD+A IW Y
Sbjct: 248 WPLYLSTKNTILKKYDGRFKDIFQENYETKWRAKFDDAGIWNMEPYFPPLCPNHFCCGRY 307
Query: 311 EHRLIDDMVXQAIK---GSMWXC 370
EHRLIDDMV A+K G +W C
Sbjct: 308 EHRLIDDMVAYALKSEGGYVWAC 330
>UniRef50_A5N5L9 Cluster: Idh; n=2; Bacteria|Rep: Idh - Clostridium
kluyveri DSM 555
Length = 401
Score = 98.7 bits (235), Expect = 5e-20
Identities = 49/125 (39%), Positives = 76/125 (60%), Gaps = 3/125 (2%)
Frame = +2
Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184
++ V +PGK+ELV+T+ G R+ L+ PGV MGM+N D+SI SFA S F AL
Sbjct: 144 EYKVEEPGKMELVFTSEKGEETRQTLHVFNGPGVVMGMHNLDKSIESFARSCFNYALDMN 203
Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---G 355
L+ ++++ +T F+ FQE++ ++Y KF++A I Y + LIDD V + +K G
Sbjct: 204 QNLWFASKDTISKTYDHRFKDIFQEIYDTEYDAKFNDAGIEYFYTLIDDAVARVVKSEGG 263
Query: 356 SMWXC 370
+W C
Sbjct: 264 FIWAC 268
>UniRef50_Q4VCC2 Cluster: Isocitrate dehydrogenase; n=4;
Eukaryota|Rep: Isocitrate dehydrogenase - Saltugilia
latimeri
Length = 158
Score = 83.8 bits (198), Expect = 2e-15
Identities = 38/70 (54%), Positives = 49/70 (70%)
Frame = +2
Query: 125 TDESIRSFAHSSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKI 304
TDESI SFA +S A +KKWPLYLST+ + F+ FQEV++ +K+K++ A I
Sbjct: 1 TDESIYSFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEKSWKSKYEAAGI 60
Query: 305 WYEHRLIDDM 334
WYEHRLIDDM
Sbjct: 61 WYEHRLIDDM 70
>UniRef50_A3JDN6 Cluster: Isocitrate dehydrogenase; n=5;
Gammaproteobacteria|Rep: Isocitrate dehydrogenase -
Marinobacter sp. ELB17
Length = 582
Score = 37.1 bits (82), Expect = 0.18
Identities = 22/101 (21%), Positives = 45/101 (44%)
Frame = +2
Query: 26 GKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLST 205
G V+L++ + G D K + N ++++AH FQ A+ +K YL
Sbjct: 163 GVVKLLFVGKSGNPVELHRRDVKKGDPWLLATNDIADVKAWAHRFFQRAIDEKRDAYLGL 222
Query: 206 EEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLID 328
++ + + +++ +YK +F+ I Y + L+D
Sbjct: 223 KDTVIAGYDGVMRAAIEAIYKQEYKKQFEAVGINYYYELVD 263
>UniRef50_Q3ISP1 Cluster: DNA polymerase; n=1; Natronomonas pharaonis
DSM 2160|Rep: DNA polymerase - Natronomonas pharaonis
(strain DSM 2160 / ATCC 35678)
Length = 1740
Score = 32.7 bits (71), Expect = 3.8
Identities = 27/103 (26%), Positives = 49/103 (47%), Gaps = 1/103 (0%)
Frame = +2
Query: 128 DESI-RSFAHSSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKI 304
DE+I +SF S ++ L+ EE+ F+ F++ ++ FQ+ K ++ I
Sbjct: 1478 DEAIEQSFEIESHINDAYDRFAERLNAEEHRFQI---EFEKLYRRFFQAGKKKRYAGHII 1534
Query: 305 WYEHRLIDDMVXQAIKGSMWXCLXLAXTTTDMSVRYLSLTVTG 433
W E + +DD+ I G + +A T ++ L + VTG
Sbjct: 1535 WKEGKEVDDL---DITGFEYQRSDIAPITKEVQREVLEMIVTG 1574
>UniRef50_A7AG60 Cluster: Putative uncharacterized protein; n=1;
Parabacteroides merdae ATCC 43184|Rep: Putative
uncharacterized protein - Parabacteroides merdae ATCC
43184
Length = 493
Score = 32.3 bits (70), Expect = 5.0
Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +2
Query: 131 ESIRSFAHSSFQVALQKKWPLYLSTEE-YHFETLRW-SFQRHFQEVFQSDYKTK 286
+++ + +F+ A+QK+W L + E Y F +RW ++ +E FQ ++ K
Sbjct: 416 DNLSGLSKETFRQAIQKEWTLETTHERIYRFNLVRWGTYLTVMKEYFQKNFPEK 469
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 427,832,104
Number of Sequences: 1657284
Number of extensions: 7791069
Number of successful extensions: 18319
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 17886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18300
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 23604537544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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