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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0671.Seq
         (449 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom...   138   5e-34
SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ...    27   1.8  
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb...    26   2.3  
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ...    26   3.1  
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc...    26   3.1  
SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual           25   4.1  
SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ...    25   4.1  
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S...    25   7.1  
SPAC1399.05c |||transcription factor, zf-fungal binuclear cluste...    24   9.4  

>SPAC6G10.08 |idp1||isocitrate dehydrogenase
           Idp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 418

 Score =  138 bits (333), Expect = 5e-34
 Identities = 68/127 (53%), Positives = 86/127 (67%), Gaps = 5/127 (3%)
 Frame = +2

Query: 5   DFVVPKPGKVELVYTTRDGTTERRV--LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQ 178
           D V   PGK+EL +T +   + +    +Y+    GVAM MYNTD+SIR FAHSSFQ+ALQ
Sbjct: 153 DLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMSMYNTDDSIRGFAHSSFQMALQ 212

Query: 179 KKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK-- 352
           KK PLYLST+    +     F+  FQEV++SDYK KF+E  +WY+HRLIDDMV QAIK  
Sbjct: 213 KKMPLYLSTKNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSN 272

Query: 353 -GSMWXC 370
            G +W C
Sbjct: 273 GGFVWAC 279



 Score = 29.5 bits (63), Expect = 0.25
 Identities = 13/23 (56%), Positives = 14/23 (60%)
 Frame = +1

Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447
           C NYDG V    VA  Y SLG+M
Sbjct: 279 CKNYDGDVMSDVVAQAYGSLGLM 301


>SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 388

 Score = 26.6 bits (56), Expect = 1.8
 Identities = 10/35 (28%), Positives = 19/35 (54%)
 Frame = +2

Query: 128 DESIRSFAHSSFQVALQKKWPLYLSTEEYHFETLR 232
           DE +   ++S  ++ +QK WP +   +EY    +R
Sbjct: 38  DEKVSFESYSPLKLMIQKTWPRFQEHKEYRESFIR 72


>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 26.2 bits (55), Expect = 2.3
 Identities = 22/98 (22%), Positives = 46/98 (46%), Gaps = 8/98 (8%)
 Frame = +2

Query: 8   FVVPKPGKVELVYTTRDGTTERRVLYD-XKTPGVAM----GMYNTDESIRSFAHSSFQVA 172
           F++ + G+V +   T      RR+LYD  K  G  +     + N ++ I +    +  V+
Sbjct: 130 FLLKEKGQVAIYDATNGTRRTRRILYDRFKNCGFKILFIESLCNKEDVINANIQEAIHVS 189

Query: 173 LQ-KKWPLYLSTEEY--HFETLRWSFQRHFQEVFQSDY 277
            + + W L ++ +EY    + L+     H++ + + DY
Sbjct: 190 EEFRNWDLEMAEKEYCRRIDILKC----HYETIDEKDY 223



 Score = 24.6 bits (51), Expect = 7.1
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +1

Query: 115 HVQYRRIDSVLCPFQFSGSFTEKMASI 195
           HV     DSV+ PF+  GS T  M+S+
Sbjct: 309 HVLSCMEDSVMSPFRELGSVTSSMSSL 335


>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 621

 Score = 25.8 bits (54), Expect = 3.1
 Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -3

Query: 213 YSSVDRYRGHFF-CKAT*KLEWAKDRIDSSVLYMPIAT 103
           Y+ V  +  +F   KAT K EW K++ID   L   I T
Sbjct: 286 YTDVKSHSNNFAKFKATEKREWVKEKIDLESLLGTIQT 323


>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
           Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 596

 Score = 25.8 bits (54), Expect = 3.1
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +3

Query: 201 LXKNTILKRYDGRFKDIFKKFFRVTTR 281
           + + T+L   DG+FKDIF K   +  R
Sbjct: 413 ITQETLLGLLDGKFKDIFDKCIIIDCR 439


>SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 604

 Score = 25.4 bits (53), Expect = 4.1
 Identities = 13/40 (32%), Positives = 24/40 (60%)
 Frame = -1

Query: 338 PPCRQLNDARTISWLHQT*SCSHSEKLLENVFETTIVTFQ 219
           PP + LN A  +S L++  + +  + LLE+ F   I++F+
Sbjct: 553 PPRKLLNKAHLLSCLNKLDAGTDEQILLEDEFTRIIMSFK 592


>SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 324

 Score = 25.4 bits (53), Expect = 4.1
 Identities = 9/25 (36%), Positives = 13/25 (52%)
 Frame = +2

Query: 242 QRHFQEVFQSDYKTKFDEAKIWYEH 316
           QR F + F   Y T+   A + Y+H
Sbjct: 68  QREFNDTFSPKYSTRLSSAGLIYKH 92


>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
           Sad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 10/30 (33%), Positives = 16/30 (53%)
 Frame = -1

Query: 182 FSVKLPENWNGQRTESIRRYCTCP*QRPEF 93
           F V+L ++W      ++R+Y T P   P F
Sbjct: 287 FEVELNDDWKFFIESTVRKYLTDPVSMPNF 316


>SPAC1399.05c |||transcription factor, zf-fungal binuclear cluster
           type|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 529

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 8/26 (30%), Positives = 16/26 (61%)
 Frame = +2

Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQE 259
           KW L +S ++YH  T   ++ + +Q+
Sbjct: 464 KWLLVISVDQYHLATKFANYLKEYQK 489


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,798,970
Number of Sequences: 5004
Number of extensions: 34121
Number of successful extensions: 106
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 166231220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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