BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0671.Seq (449 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 138 5e-34 SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 27 1.8 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 26 2.3 SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 26 3.1 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 3.1 SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual 25 4.1 SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ... 25 4.1 SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 25 7.1 SPAC1399.05c |||transcription factor, zf-fungal binuclear cluste... 24 9.4 >SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 138 bits (333), Expect = 5e-34 Identities = 68/127 (53%), Positives = 86/127 (67%), Gaps = 5/127 (3%) Frame = +2 Query: 5 DFVVPKPGKVELVYTTRDGTTERRV--LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQ 178 D V PGK+EL +T + + + +Y+ GVAM MYNTD+SIR FAHSSFQ+ALQ Sbjct: 153 DLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMSMYNTDDSIRGFAHSSFQMALQ 212 Query: 179 KKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK-- 352 KK PLYLST+ + F+ FQEV++SDYK KF+E +WY+HRLIDDMV QAIK Sbjct: 213 KKMPLYLSTKNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSN 272 Query: 353 -GSMWXC 370 G +W C Sbjct: 273 GGFVWAC 279 Score = 29.5 bits (63), Expect = 0.25 Identities = 13/23 (56%), Positives = 14/23 (60%) Frame = +1 Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447 C NYDG V VA Y SLG+M Sbjct: 279 CKNYDGDVMSDVVAQAYGSLGLM 301 >SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 26.6 bits (56), Expect = 1.8 Identities = 10/35 (28%), Positives = 19/35 (54%) Frame = +2 Query: 128 DESIRSFAHSSFQVALQKKWPLYLSTEEYHFETLR 232 DE + ++S ++ +QK WP + +EY +R Sbjct: 38 DEKVSFESYSPLKLMIQKTWPRFQEHKEYRESFIR 72 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 26.2 bits (55), Expect = 2.3 Identities = 22/98 (22%), Positives = 46/98 (46%), Gaps = 8/98 (8%) Frame = +2 Query: 8 FVVPKPGKVELVYTTRDGTTERRVLYD-XKTPGVAM----GMYNTDESIRSFAHSSFQVA 172 F++ + G+V + T RR+LYD K G + + N ++ I + + V+ Sbjct: 130 FLLKEKGQVAIYDATNGTRRTRRILYDRFKNCGFKILFIESLCNKEDVINANIQEAIHVS 189 Query: 173 LQ-KKWPLYLSTEEY--HFETLRWSFQRHFQEVFQSDY 277 + + W L ++ +EY + L+ H++ + + DY Sbjct: 190 EEFRNWDLEMAEKEYCRRIDILKC----HYETIDEKDY 223 Score = 24.6 bits (51), Expect = 7.1 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 115 HVQYRRIDSVLCPFQFSGSFTEKMASI 195 HV DSV+ PF+ GS T M+S+ Sbjct: 309 HVLSCMEDSVMSPFRELGSVTSSMSSL 335 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 25.8 bits (54), Expect = 3.1 Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -3 Query: 213 YSSVDRYRGHFF-CKAT*KLEWAKDRIDSSVLYMPIAT 103 Y+ V + +F KAT K EW K++ID L I T Sbjct: 286 YTDVKSHSNNFAKFKATEKREWVKEKIDLESLLGTIQT 323 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 3.1 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +3 Query: 201 LXKNTILKRYDGRFKDIFKKFFRVTTR 281 + + T+L DG+FKDIF K + R Sbjct: 413 ITQETLLGLLDGKFKDIFDKCIIIDCR 439 >SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 25.4 bits (53), Expect = 4.1 Identities = 13/40 (32%), Positives = 24/40 (60%) Frame = -1 Query: 338 PPCRQLNDARTISWLHQT*SCSHSEKLLENVFETTIVTFQ 219 PP + LN A +S L++ + + + LLE+ F I++F+ Sbjct: 553 PPRKLLNKAHLLSCLNKLDAGTDEQILLEDEFTRIIMSFK 592 >SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 25.4 bits (53), Expect = 4.1 Identities = 9/25 (36%), Positives = 13/25 (52%) Frame = +2 Query: 242 QRHFQEVFQSDYKTKFDEAKIWYEH 316 QR F + F Y T+ A + Y+H Sbjct: 68 QREFNDTFSPKYSTRLSSAGLIYKH 92 >SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 24.6 bits (51), Expect = 7.1 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = -1 Query: 182 FSVKLPENWNGQRTESIRRYCTCP*QRPEF 93 F V+L ++W ++R+Y T P P F Sbjct: 287 FEVELNDDWKFFIESTVRKYLTDPVSMPNF 316 >SPAC1399.05c |||transcription factor, zf-fungal binuclear cluster type|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 24.2 bits (50), Expect = 9.4 Identities = 8/26 (30%), Positives = 16/26 (61%) Frame = +2 Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQE 259 KW L +S ++YH T ++ + +Q+ Sbjct: 464 KWLLVISVDQYHLATKFANYLKEYQK 489 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,798,970 Number of Sequences: 5004 Number of extensions: 34121 Number of successful extensions: 106 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 105 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 166231220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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