BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0671.Seq (449 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 133 5e-32 At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 128 2e-30 At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 120 6e-28 At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR... 29 1.5 At3g11730.1 68416.m01439 Ras-related GTP-binding protein, putati... 27 7.7 At1g12170.1 68414.m01409 F-box family protein contains F-box dom... 27 7.7 >At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase (NADP+) [Nicotiana tabacum] GI:3021512; contains Pfam domain PF00180: dehydrogenase, isocitrate/isopropylmalate family Length = 485 Score = 133 bits (322), Expect = 5e-32 Identities = 64/126 (50%), Positives = 85/126 (67%), Gaps = 4/126 (3%) Frame = +2 Query: 5 DFVVPKPGKVELVYTTRDGTTERRV-LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181 D V+ PGK+++V+ DG + +YD K PGVA+ MYN DESIR+FA SS +AL K Sbjct: 215 DTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESIRAFAESSMAMALTK 274 Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352 KWPLYLST+ + F+ FQEV+++++K KF+E IWYEHRLIDDMV A+K Sbjct: 275 KWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEHSIWYEHRLIDDMVAYAVKSEG 334 Query: 353 GSMWXC 370 G +W C Sbjct: 335 GYVWAC 340 Score = 29.9 bits (64), Expect = 0.83 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +1 Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447 C NYDG V +A G+ SLG+M Sbjct: 340 CKNYDGDVQSDLLAQGFGSLGLM 362 >At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase SP|Q40345 from [Medicago sativa] Length = 410 Score = 128 bits (309), Expect = 2e-30 Identities = 62/125 (49%), Positives = 81/125 (64%), Gaps = 3/125 (2%) Frame = +2 Query: 5 DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184 D V+ PGK+ + + +DG TE V GVAM MYNTDESIR+FA +S A +KK Sbjct: 145 DAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIRAFADASMNTAYEKK 204 Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---G 355 WPLYLST+ + F+ FQEV+++ +K+K+D A IWYEHRLIDDMV A+K G Sbjct: 205 WPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDAAGIWYEHRLIDDMVAYALKSEGG 264 Query: 356 SMWXC 370 +W C Sbjct: 265 YVWAC 269 Score = 32.3 bits (70), Expect = 0.16 Identities = 13/23 (56%), Positives = 16/23 (69%) Frame = +1 Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447 C NYDG V F+A G+ SLG+M Sbjct: 269 CKNYDGDVQSDFLAQGFGSLGLM 291 >At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to NADP-isocitrate dehydrogenase GI:5764653 from [Citrus limon]; Nicotiana tabacum SP|P50218 Length = 416 Score = 120 bits (288), Expect = 6e-28 Identities = 58/126 (46%), Positives = 84/126 (66%), Gaps = 4/126 (3%) Frame = +2 Query: 5 DFVVPKPGKVELVYTTRDGTTERRV-LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181 D +V +PGK++LV+ + + +++ GVA+ MYNTDESIR+FA SS A QK Sbjct: 145 DLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMYNTDESIRAFAESSMYTAYQK 204 Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352 KWPLYLST+ + F+ FQEV+++++++K++ A IWYEHRLIDDMV A+K Sbjct: 205 KWPLYLSTKNTILKIYDGRFKDIFQEVYEANWRSKYEAAGIWYEHRLIDDMVAYAMKSEG 264 Query: 353 GSMWXC 370 G +W C Sbjct: 265 GYVWAC 270 Score = 35.1 bits (77), Expect = 0.022 Identities = 15/23 (65%), Positives = 16/23 (69%) Frame = +1 Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447 C NYDG V F+A GY SLGMM Sbjct: 270 CKNYDGDVQSDFLAQGYGSLGMM 292 >At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 997 Score = 29.1 bits (62), Expect = 1.5 Identities = 19/68 (27%), Positives = 33/68 (48%) Frame = +2 Query: 155 SSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDM 334 +S + + W + T +H +R SF HF++ F ++ T FD+ +I I Sbjct: 2 ASSSSSASRTWRYRVFTS-FHGSDVRTSFLSHFRKQFNNNGITMFDDQRI-LRGETISPA 59 Query: 335 VXQAIKGS 358 + QAI+ S Sbjct: 60 LTQAIRES 67 >At3g11730.1 68416.m01439 Ras-related GTP-binding protein, putative similar to Rab1-like small GTP-binding protein GI:4096662 from [Petunia x hybrida] Length = 205 Score = 26.6 bits (56), Expect = 7.7 Identities = 9/19 (47%), Positives = 11/19 (57%) Frame = -3 Query: 228 NVSKWYSSVDRYRGHFFCK 172 NV +W S +DRY CK Sbjct: 98 NVKQWLSEIDRYANESVCK 116 >At1g12170.1 68414.m01409 F-box family protein contains F-box domain Pfam:PF00646 Length = 364 Score = 26.6 bits (56), Expect = 7.7 Identities = 12/24 (50%), Positives = 12/24 (50%) Frame = +2 Query: 188 PLYLSTEEYHFETLRWSFQRHFQE 259 PLY S E Y FET W F E Sbjct: 167 PLYRSVEIYTFETNGWKSMNTFSE 190 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,351,662 Number of Sequences: 28952 Number of extensions: 177652 Number of successful extensions: 459 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 447 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 455 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 732537840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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