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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0671.Seq
         (449 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   133   5e-32
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   128   2e-30
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   120   6e-28
At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR...    29   1.5  
At3g11730.1 68416.m01439 Ras-related GTP-binding protein, putati...    27   7.7  
At1g12170.1 68414.m01409 F-box family protein contains F-box dom...    27   7.7  

>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  133 bits (322), Expect = 5e-32
 Identities = 64/126 (50%), Positives = 85/126 (67%), Gaps = 4/126 (3%)
 Frame = +2

Query: 5   DFVVPKPGKVELVYTTRDGTTERRV-LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181
           D V+  PGK+++V+   DG     + +YD K PGVA+ MYN DESIR+FA SS  +AL K
Sbjct: 215 DTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESIRAFAESSMAMALTK 274

Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352
           KWPLYLST+    +     F+  FQEV+++++K KF+E  IWYEHRLIDDMV  A+K   
Sbjct: 275 KWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEHSIWYEHRLIDDMVAYAVKSEG 334

Query: 353 GSMWXC 370
           G +W C
Sbjct: 335 GYVWAC 340



 Score = 29.9 bits (64), Expect = 0.83
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = +1

Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447
           C NYDG V    +A G+ SLG+M
Sbjct: 340 CKNYDGDVQSDLLAQGFGSLGLM 362


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  128 bits (309), Expect = 2e-30
 Identities = 62/125 (49%), Positives = 81/125 (64%), Gaps = 3/125 (2%)
 Frame = +2

Query: 5   DFVVPKPGKVELVYTTRDGTTERRVLYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQKK 184
           D V+  PGK+ + +  +DG TE  V       GVAM MYNTDESIR+FA +S   A +KK
Sbjct: 145 DAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIRAFADASMNTAYEKK 204

Query: 185 WPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK---G 355
           WPLYLST+    +     F+  FQEV+++ +K+K+D A IWYEHRLIDDMV  A+K   G
Sbjct: 205 WPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDAAGIWYEHRLIDDMVAYALKSEGG 264

Query: 356 SMWXC 370
            +W C
Sbjct: 265 YVWAC 269



 Score = 32.3 bits (70), Expect = 0.16
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = +1

Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447
           C NYDG V   F+A G+ SLG+M
Sbjct: 269 CKNYDGDVQSDFLAQGFGSLGLM 291


>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  120 bits (288), Expect = 6e-28
 Identities = 58/126 (46%), Positives = 84/126 (66%), Gaps = 4/126 (3%)
 Frame = +2

Query: 5   DFVVPKPGKVELVYTTRDGTTERRV-LYDXKTPGVAMGMYNTDESIRSFAHSSFQVALQK 181
           D +V +PGK++LV+     + +    +++    GVA+ MYNTDESIR+FA SS   A QK
Sbjct: 145 DLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMYNTDESIRAFAESSMYTAYQK 204

Query: 182 KWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDMVXQAIK--- 352
           KWPLYLST+    +     F+  FQEV+++++++K++ A IWYEHRLIDDMV  A+K   
Sbjct: 205 KWPLYLSTKNTILKIYDGRFKDIFQEVYEANWRSKYEAAGIWYEHRLIDDMVAYAMKSEG 264

Query: 353 GSMWXC 370
           G +W C
Sbjct: 265 GYVWAC 270



 Score = 35.1 bits (77), Expect = 0.022
 Identities = 15/23 (65%), Positives = 16/23 (69%)
 Frame = +1

Query: 379 CXNYDGYVSPIFVAHGYXSLGMM 447
           C NYDG V   F+A GY SLGMM
Sbjct: 270 CKNYDGDVQSDFLAQGYGSLGMM 292


>At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 997

 Score = 29.1 bits (62), Expect = 1.5
 Identities = 19/68 (27%), Positives = 33/68 (48%)
 Frame = +2

Query: 155 SSFQVALQKKWPLYLSTEEYHFETLRWSFQRHFQEVFQSDYKTKFDEAKIWYEHRLIDDM 334
           +S   +  + W   + T  +H   +R SF  HF++ F ++  T FD+ +I      I   
Sbjct: 2   ASSSSSASRTWRYRVFTS-FHGSDVRTSFLSHFRKQFNNNGITMFDDQRI-LRGETISPA 59

Query: 335 VXQAIKGS 358
           + QAI+ S
Sbjct: 60  LTQAIRES 67


>At3g11730.1 68416.m01439 Ras-related GTP-binding protein, putative
           similar to Rab1-like small GTP-binding protein
           GI:4096662 from [Petunia x hybrida]
          Length = 205

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 9/19 (47%), Positives = 11/19 (57%)
 Frame = -3

Query: 228 NVSKWYSSVDRYRGHFFCK 172
           NV +W S +DRY     CK
Sbjct: 98  NVKQWLSEIDRYANESVCK 116


>At1g12170.1 68414.m01409 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 364

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 12/24 (50%), Positives = 12/24 (50%)
 Frame = +2

Query: 188 PLYLSTEEYHFETLRWSFQRHFQE 259
           PLY S E Y FET  W     F E
Sbjct: 167 PLYRSVEIYTFETNGWKSMNTFSE 190


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,351,662
Number of Sequences: 28952
Number of extensions: 177652
Number of successful extensions: 459
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 455
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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