BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0660.Seq (449 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC29A3.12 |rps902|rps9-2, rps9b|40S ribosomal protein S9|Schiz... 109 1e-25 SPAC24H6.07 |rps901|rps9-1, rps9a|40S ribosomal protein S9|Schiz... 109 2e-25 SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 28 0.58 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 1.8 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 26 2.3 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 26 2.3 SPBC3B9.21 |dcp1||mRNA decapping complex subunit Dcp1|Schizosacc... 25 4.1 SPAC17A2.04c |||HSP chaperone complex subunit |Schizosaccharomyc... 25 5.4 SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces po... 24 9.4 SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosacchar... 24 9.4 SPAC4F10.12 |fta1|sma1|Sim4 and Mal2 associated |Schizosaccharom... 24 9.4 SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 24 9.4 SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor E|S... 24 9.4 >SPBC29A3.12 |rps902|rps9-2, rps9b|40S ribosomal protein S9|Schizosaccharomyces pombe|chr 2|||Manual Length = 192 Score = 109 bits (263), Expect = 1e-25 Identities = 51/65 (78%), Positives = 59/65 (90%) Frame = -2 Query: 448 RKAAREXLTLEKKXPKRLFEGNALXRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQTQ 269 R+AARE LTL++K PKRLFEGNA+ RRLVR+G+LDE +MKLDYVL L+IEDFLERRLQTQ Sbjct: 53 RRAARELLTLDEKDPKRLFEGNAIIRRLVRLGILDESRMKLDYVLALRIEDFLERRLQTQ 112 Query: 268 VFKAG 254 VFK G Sbjct: 113 VFKLG 117 Score = 109 bits (262), Expect = 2e-25 Identities = 49/64 (76%), Positives = 56/64 (87%) Frame = -1 Query: 254 LAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRPGRVKRKNL 75 LAKSIHHAR+LI QRHIRV KQ+VN+PSF+VRLD+ KHIDF+L SP+GGGRPGR KRK L Sbjct: 118 LAKSIHHARVLIFQRHIRVGKQIVNVPSFVVRLDAQKHIDFALSSPYGGGRPGRCKRKRL 177 Query: 74 RKGQ 63 R Q Sbjct: 178 RSQQ 181 >SPAC24H6.07 |rps901|rps9-1, rps9a|40S ribosomal protein S9|Schizosaccharomyces pombe|chr 1|||Manual Length = 191 Score = 109 bits (262), Expect = 2e-25 Identities = 51/65 (78%), Positives = 59/65 (90%) Frame = -2 Query: 448 RKAAREXLTLEKKXPKRLFEGNALXRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQTQ 269 R+AARE LTL++K PKRLFEGNA+ RRLVR+G+LDE +MKLDYVL L+IEDFLERRLQTQ Sbjct: 53 RRAARELLTLDEKDPKRLFEGNAIIRRLVRLGILDETRMKLDYVLALRIEDFLERRLQTQ 112 Query: 268 VFKAG 254 VFK G Sbjct: 113 VFKLG 117 Score = 108 bits (259), Expect = 4e-25 Identities = 48/61 (78%), Positives = 55/61 (90%) Frame = -1 Query: 254 LAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRPGRVKRKNL 75 LAKSIHHAR+LI QRHIRV KQ+VN+PSF+VRLD+ KHIDF+L SP+GGGRPGR KRK L Sbjct: 118 LAKSIHHARVLIFQRHIRVGKQIVNVPSFVVRLDTQKHIDFALSSPYGGGRPGRCKRKRL 177 Query: 74 R 72 R Sbjct: 178 R 178 >SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pombe|chr 2|||Manual Length = 736 Score = 28.3 bits (60), Expect = 0.58 Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 3/52 (5%) Frame = -2 Query: 358 IGVLDEKQMKLDYVLGLKIEDFLERRLQTQV---FKAGWRSPSIMPEF*SGK 212 +G++ EK M+LD V G K+E ++RR + +A W +I E+ S K Sbjct: 101 MGLVGEK-MELDAVKGRKVESRVDRRKLEKAERKIRAKWEKRTIKAEYESSK 151 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 26.6 bits (56), Expect = 1.8 Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 4/77 (5%) Frame = +2 Query: 74 GGSSS*HVQDDLHRMEISERSQCACQSPGAQ*MMG---CSQLACGHEY-AFAGSKFWHDG 241 G S S ++++L ++ +S G Q +G + L + + + AG+ ++D Sbjct: 2027 GHSDSAWIEENLSDLKFLLEKSLENESVGVQSAIGNFVSTILTLSNTHPSIAGNPIFNDI 2086 Query: 242 WTSPASFEHLRLQTTLQ 292 WTS AS+ RLQ+ Q Sbjct: 2087 WTSIASWTERRLQSCSQ 2103 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 26.2 bits (55), Expect = 2.3 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = -1 Query: 335 DETRLCAWSED*GLLGASSADAGVQSWLAKSIHHARILIRQ 213 D L W ++ +L S G+QS+++ S A+ L R+ Sbjct: 129 DRQPLSTWQQNISVLSESPPQNGIQSYISSSEQAAQALTRK 169 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 26.2 bits (55), Expect = 2.3 Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -2 Query: 358 IGVLDEKQMKLDYV-LGLKIEDFLERRLQTQVFKAGW 251 IG + KQ+ ++ L +ED+LE + +F+ GW Sbjct: 879 IGKIIAKQLNFKFLDLDELLEDYLEMPIAEVIFRMGW 915 >SPBC3B9.21 |dcp1||mRNA decapping complex subunit Dcp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 127 Score = 25.4 bits (53), Expect = 4.1 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = -1 Query: 275 DAGVQSWLAKSIHHARILIRQRHIRVRKQVVN 180 D G Q WL SI L++ + RV ++N Sbjct: 39 DVGSQKWLKTSIEGTFFLVKDQRARVGYVILN 70 >SPAC17A2.04c |||HSP chaperone complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 358 Score = 25.0 bits (52), Expect = 5.4 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = -3 Query: 180 HPIIYCAPGLWQAH*LLSEISIRWRSSWTCQEEE 79 HP + GL Q H + ++ W SSW ++E Sbjct: 321 HPKVTLIDGLVQLHVVPHHLASDWISSWKKNKQE 354 >SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 24.2 bits (50), Expect = 9.4 Identities = 8/33 (24%), Positives = 17/33 (51%) Frame = -3 Query: 156 GLWQAH*LLSEISIRWRSSWTCQEEEPPQGTGW 58 G + A +L ++S W+ +W +E++ W Sbjct: 383 GRYIAQRILGDLSEEWKDAWRWREDDKASELKW 415 >SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 24.2 bits (50), Expect = 9.4 Identities = 16/57 (28%), Positives = 22/57 (38%) Frame = -2 Query: 448 RKAAREXLTLEKKXPKRLFEGNALXRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRL 278 R+ A L+ P RL N L +L +G+L K D + RRL Sbjct: 55 RQLAHRLSLLDPTDPFRLQYENLLLEKLFDMGILPSKSKMSDIENKANVSAICRRRL 111 >SPAC4F10.12 |fta1|sma1|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 280 Score = 24.2 bits (50), Expect = 9.4 Identities = 11/37 (29%), Positives = 21/37 (56%) Frame = -2 Query: 334 MKLDYVLGLKIEDFLERRLQTQVFKAGWRSPSIMPEF 224 +++D L + +E FL++ TQ+ +P I+ EF Sbjct: 132 VRMDGALWMAVEQFLQQEFDTQILPC-LIAPEILLEF 167 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 24.2 bits (50), Expect = 9.4 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = +3 Query: 390 SNNLLGXFFSRVRXSR 437 +NNL+G FF R+ SR Sbjct: 314 TNNLIGGFFQRLEASR 329 >SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor E|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 24.2 bits (50), Expect = 9.4 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = -1 Query: 248 KSIHHARILIRQRHIRVRKQVVNIPSFIVRL 156 +S+ H R L +H+R+ N P+ I +L Sbjct: 306 RSLDHLRTLENLKHLRITLSYFNKPTDIAKL 336 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,715,159 Number of Sequences: 5004 Number of extensions: 33079 Number of successful extensions: 85 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 166231220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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