BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0654.Seq (449 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g43930.1 68415.m05460 protein kinase family protein contains ... 28 2.5 At4g10890.1 68417.m01772 expressed protein 27 4.4 At3g49060.1 68416.m05360 protein kinase family protein / U-box d... 27 4.4 At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 27 5.9 At5g38320.1 68418.m04625 expressed protein ; expression support... 27 7.7 At3g11570.1 68416.m01413 expressed protein similar to At5g06230 27 7.7 >At2g43930.1 68415.m05460 protein kinase family protein contains similarity to NPK1-related protein kinase 2 GI:2342425 from [Arabidopsis thaliana] Length = 204 Score = 28.3 bits (60), Expect = 2.5 Identities = 13/39 (33%), Positives = 19/39 (48%) Frame = +1 Query: 208 EPRSYSIIPCTKYSSRFLARFEHSNLFKVKLSAHLDTHR 324 EP ++ C K S F + FEH LFK + + H+ Sbjct: 63 EPHIVLLLQCRKKSWCFASEFEHLKLFKGYIDEDEERHK 101 >At4g10890.1 68417.m01772 expressed protein Length = 527 Score = 27.5 bits (58), Expect = 4.4 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = -1 Query: 113 RKTNISESICQRCFHQS-RTKVRGSKAIRYRPSSN 12 ++T I +C RC+H S R K+R S R S++ Sbjct: 194 KQTKICSRVCSRCYHYSMRQKLRHSLHTRILKSNS 228 >At3g49060.1 68416.m05360 protein kinase family protein / U-box domain-containing protein contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 805 Score = 27.5 bits (58), Expect = 4.4 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = +1 Query: 76 HLWQMLSLMFVLRRSKNFTSNVAIRMPPVIPINHYLGVLKTNKIE 210 H+W + + R+ N SN MPP++ ++ K+ K+E Sbjct: 162 HIWFLCKGYLIFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLE 206 >At2g36350.1 68415.m04461 protein kinase, putative similar to protein kinase KIPK (KCBP-interacting protein kinase) [Arabidopsis thaliana] gi|7716430|gb|AAF68383 Length = 949 Score = 27.1 bits (57), Expect = 5.9 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +2 Query: 263 PALSTLICSK*NCRPTSTLTEEH 331 P+ S L+C K +C ST TE H Sbjct: 391 PSASQLLCQKCHCAVKSTSTENH 413 >At5g38320.1 68418.m04625 expressed protein ; expression supported by MPSS Length = 212 Score = 26.6 bits (56), Expect = 7.7 Identities = 14/47 (29%), Positives = 23/47 (48%), Gaps = 1/47 (2%) Frame = +1 Query: 115 RSKNFTSNVAIRMPPVIPINHYLGVLKTNKIEPRSYSI-IPCTKYSS 252 RSK + P +IPI + VL+ +I + Y + +P Y+S Sbjct: 85 RSKTDKYKTGLPRPEIIPIEDFEPVLEIEEIGDQEYEVKLPLLPYNS 131 >At3g11570.1 68416.m01413 expressed protein similar to At5g06230 Length = 427 Score = 26.6 bits (56), Expect = 7.7 Identities = 17/58 (29%), Positives = 28/58 (48%) Frame = +1 Query: 265 RFEHSNLFKVKLSAHLDTHRRAPR*DFDIEPAFFRTPAHRRYAPQTCXYXRXCGAPDS 438 R+EHS + + ++ +L R+ D P+ +R P APQ C + G PD+ Sbjct: 360 RYEHSKVKFLNIT-YLTEFRK------DAHPSRYREPGTPEDAPQDCSHWCLPGVPDT 410 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,737,906 Number of Sequences: 28952 Number of extensions: 164166 Number of successful extensions: 355 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 351 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 355 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 732537840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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