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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0644.Seq
         (489 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S ...    77   6e-15
At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) simi...    77   6e-15
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)            74   5e-14
At2g02640.1 68415.m00203 DC1 domain-containing protein   contain...    31   0.42 
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r...    28   3.9  
At1g32190.1 68414.m03959 expressed protein                             27   5.1  
At5g58840.1 68418.m07373 subtilase family protein contains simil...    27   6.8  
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf...    27   6.8  
At2g39850.1 68415.m04894 subtilase family protein contains simil...    27   9.0  

>At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S
           ribosomal protein L18, Arabidopsis thaliana,
           SWISSPROT:RL18_ARATH
          Length = 187

 Score = 77.0 bits (181), Expect = 6e-15
 Identities = 37/57 (64%), Positives = 45/57 (78%)
 Frame = +1

Query: 256 AVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALSAPTGXKT 426
           AV+VGT+T+D+R+++IP M V AL  TE+ARARI  AGGE LTFDQLAL AP G  T
Sbjct: 79  AVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNT 135



 Score = 54.0 bits (124), Expect = 5e-08
 Identities = 27/71 (38%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
 Frame = +2

Query: 20  MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 196
           MGID I     +K +RT  KS D+               +N+ FN ++L+RLFMS++N+ 
Sbjct: 1   MGIDLIAGGKSKKTKRTAPKSDDVYLKLLVKLYRFLVRRSNSNFNAVILKRLFMSKVNKA 60

Query: 197 PISVSRLARHM 229
           P+S+SRL   M
Sbjct: 61  PLSLSRLVEFM 71


>At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) similar
           to GB:P42791
          Length = 187

 Score = 77.0 bits (181), Expect = 6e-15
 Identities = 37/57 (64%), Positives = 45/57 (78%)
 Frame = +1

Query: 256 AVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALSAPTGXKT 426
           AV+VGT+T+D+R+++IP M V AL  TE+ARARI  AGGE LTFDQLAL AP G  T
Sbjct: 79  AVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNT 135



 Score = 57.2 bits (132), Expect = 6e-09
 Identities = 29/71 (40%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
 Frame = +2

Query: 20  MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 196
           MGID I     +K +RT  KS D+               TN+KFN ++L+RLFMS++N+ 
Sbjct: 1   MGIDLIAGGKSKKTKRTAPKSDDVYLKLTVKLYRFLVRRTNSKFNGVILKRLFMSKVNKA 60

Query: 197 PISVSRLARHM 229
           P+S+SRL   M
Sbjct: 61  PLSLSRLVEFM 71


>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
          Length = 135

 Score = 74.1 bits (174), Expect = 5e-14
 Identities = 36/53 (67%), Positives = 41/53 (77%)
 Frame = +1

Query: 256 AVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALSAPT 414
           AV+VGTVT+DVR+  +P +TV AL  TE ARARI  AGGE LTFDQLAL  PT
Sbjct: 26  AVIVGTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPT 78



 Score = 28.3 bits (60), Expect = 2.9
 Identities = 10/18 (55%), Positives = 16/18 (88%)
 Frame = +2

Query: 176 MSRINRPPISVSRLARHM 229
           MS++N+ P+S+SRL R+M
Sbjct: 1   MSKVNKAPLSLSRLVRYM 18


>At2g02640.1 68415.m00203 DC1 domain-containing protein   contains
           Pfam profile PF03107: DC1 domain
          Length = 627

 Score = 31.1 bits (67), Expect = 0.42
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 321 CSSCYRKSSCTHFGCWRRNSYF 386
           CS+C RKS+   + C+ RN YF
Sbjct: 423 CSACMRKSNGFGYSCYNRNCYF 444


>At3g59820.1 68416.m06675 calcium-binding mitochondrial
           protein-related contains weak similarity to
           Calcium-binding mitochondrial protein Anon-60Da
           (Swiss-Prot:P91927) [Drosophila melanogaster]
          Length = 755

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 12/36 (33%), Positives = 18/36 (50%)
 Frame = +2

Query: 149 NQIVLRRLFMSRINRPPISVSRLARHMKKPTREGLM 256
           +Q V    + S INRPP+    +  H     R+GL+
Sbjct: 34  SQTVHSHAYHSGINRPPVETKPVTEHKSFTRRDGLL 69


>At1g32190.1 68414.m03959 expressed protein
          Length = 422

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 8/17 (47%), Positives = 10/17 (58%)
 Frame = +3

Query: 321 CSSCYRKSSCTHFGCWR 371
           CSSC+ K  C    CW+
Sbjct: 359 CSSCFGKPKCPKCSCWK 375


>At5g58840.1 68418.m07373 subtilase family protein contains
           similarity to prepro-cucumisin GI:807698 from [Cucumis
           melo]; non-consensus acceptor site TT at exon 6
          Length = 713

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = -1

Query: 327 KSSHRHLRYLVQSHVICDCPHYHGI 253
           K  +RH++Y V S     CPH  G+
Sbjct: 484 KRDNRHVKYSVLSGTSMSCPHVAGV 508


>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 883

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
 Frame = +3

Query: 285 RETVQDTEDDGGCSSCY--RKSSCTHFGCWRRNSYF*SAGS*CS 410
           R+  + +E  GGC+SC    KS  T   C  R+  + S G+ CS
Sbjct: 145 RDHGECSESVGGCASCIVRSKSDITTSQCGDRDRRYTSPGNPCS 188


>At2g39850.1 68415.m04894 subtilase family protein contains
           similarity to subtilisin-like protease C1 GI:13325079
           from [Glycine max]
          Length = 774

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 11/34 (32%), Positives = 16/34 (47%)
 Frame = -1

Query: 324 SSHRHLRYLVQSHVICDCPHYHGIKPSRVGFFMW 223
           + +RHLR+ + S     CPH  G+      F  W
Sbjct: 536 NDYRHLRFNIMSGTSMACPHATGLALYLKSFKRW 569


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,243,392
Number of Sequences: 28952
Number of extensions: 206825
Number of successful extensions: 549
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 535
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 548
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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