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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0641.Seq
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...   133   1e-32
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb...    25   4.8  
SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccha...    25   6.3  
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar...    25   6.3  
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi...    25   6.3  
SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharom...    25   6.3  
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc...    25   8.4  
SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subuni...    25   8.4  
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    25   8.4  
SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyce...    25   8.4  

>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score =  133 bits (322), Expect = 1e-32
 Identities = 53/90 (58%), Positives = 69/90 (76%)
 Frame = +1

Query: 1   QKEYGFCTIDGHKEKIGNFRIEPPGLFRGRGEHPKMGKLKRRVMPEDVLINCSKDSKIPK 180
           +++Y +C +DG KEK+GNFRIEPPGLFRGRG HPK G LKRRV PE + IN  +   +P+
Sbjct: 314 EEKYKWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE 373

Query: 181 PPSGHKWREVRHDNTVTWLASWTEKFSSKL 270
           P  GH+W EV+HDNTVTWLA+W E  ++ +
Sbjct: 374 PLPGHQWAEVKHDNTVTWLATWHENINNNV 403



 Score = 67.7 bits (158), Expect = 9e-13
 Identities = 34/78 (43%), Positives = 45/78 (57%)
 Frame = +3

Query: 255 VQQQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAVAL 434
           +    KYV L   S LKG+ D +KYE +RKL   ID IR+  R D K++     QR  A+
Sbjct: 399 INNNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQRGTAM 458

Query: 435 YFIDRLALTAXNEKDDEQ 488
           Y ID  AL A NEK +++
Sbjct: 459 YLIDVFALRAGNEKGEDE 476


>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 782

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +1

Query: 187 SGHKWREVRHDNTVTWLASWTEKFS 261
           +G KW  + H NT  +L   T KFS
Sbjct: 529 TGEKWTMLHHINTTDYLNYETGKFS 553


>SPBC36.06c |spo9||farnesyl pyrophosphate
           synthetase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 351

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +1

Query: 217 DNTVTWLASWTEKFSS 264
           DN  TWL  + EKF+S
Sbjct: 260 DNKCTWLVCYAEKFAS 275


>SPAC23H4.09 |cdb4||curved DNA-binding protein
           Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 381

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +3

Query: 246 DREVQQQAKYVMLNPSSKLKGEKDWQKYE 332
           +REV    K V+LNPS   + E D   +E
Sbjct: 193 EREVIDGKKQVILNPSDSQRSEMDTFTFE 221


>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
            homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 3071

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 11/37 (29%), Positives = 16/37 (43%)
 Frame = +3

Query: 318  WQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAV 428
            W  +E A     C+  +  N   DWK +E +  Q  V
Sbjct: 1822 WADFENAAD--SCVRHLENNEETDWKFEEWRQMQDVV 1856


>SPAC959.03c |||U3 snoRNP-associated protein
           Utp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 520

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 14/38 (36%), Positives = 17/38 (44%), Gaps = 3/38 (7%)
 Frame = -3

Query: 236 NHVTVLSCLTSLHLCPEG---GFGILLSFEQLIKTSSG 132
           NH+   S +  LH CP     G G    FE +I   SG
Sbjct: 345 NHLLPSSSVVDLHYCPYEDILGIGHAKGFESIIVPGSG 382


>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 940

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +3

Query: 225 SYMVSLLDREVQQQAKYVMLNPSSKL 302
           S +++L DRE    AK +ML+  S+L
Sbjct: 279 SRLLALADREADNNAKLIMLDRISQL 304


>SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subunit
           Pmh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 318

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 15/31 (48%), Positives = 18/31 (58%)
 Frame = +3

Query: 237 SLLDREVQQQAKYVMLNPSSKLKGEKDWQKY 329
           S+L  +VQQ A+     P S L GEKD  KY
Sbjct: 233 SILLSDVQQVAEDT--TPFSPLAGEKDGSKY 261


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1364

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 8/36 (22%), Positives = 20/36 (55%)
 Frame = +3

Query: 222  YSYMVSLLDREVQQQAKYVMLNPSSKLKGEKDWQKY 329
            Y Y  +  D  V   ++++M+   ++L+ + DW ++
Sbjct: 971  YEYRKASSDMRVLMDSQFLMMKTFARLQAKGDWYEW 1006


>SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 567

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 16/37 (43%), Positives = 18/37 (48%)
 Frame = -3

Query: 182 GFGILLSFEQLIKTSSGITRLFNFPIFGCSPRPLNNP 72
           GF ILLSF   I T SG    F+     CS   +N P
Sbjct: 265 GFAILLSFNSPIWTMSGYDAPFHLS-EECSNASVNAP 300


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,850,234
Number of Sequences: 5004
Number of extensions: 34494
Number of successful extensions: 107
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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