BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0641.Seq (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 133 1e-32 SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 4.8 SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccha... 25 6.3 SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 25 6.3 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 6.3 SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharom... 25 6.3 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 25 8.4 SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subuni... 25 8.4 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 25 8.4 SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyce... 25 8.4 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 133 bits (322), Expect = 1e-32 Identities = 53/90 (58%), Positives = 69/90 (76%) Frame = +1 Query: 1 QKEYGFCTIDGHKEKIGNFRIEPPGLFRGRGEHPKMGKLKRRVMPEDVLINCSKDSKIPK 180 +++Y +C +DG KEK+GNFRIEPPGLFRGRG HPK G LKRRV PE + IN + +P+ Sbjct: 314 EEKYKWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE 373 Query: 181 PPSGHKWREVRHDNTVTWLASWTEKFSSKL 270 P GH+W EV+HDNTVTWLA+W E ++ + Sbjct: 374 PLPGHQWAEVKHDNTVTWLATWHENINNNV 403 Score = 67.7 bits (158), Expect = 9e-13 Identities = 34/78 (43%), Positives = 45/78 (57%) Frame = +3 Query: 255 VQQQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAVAL 434 + KYV L S LKG+ D +KYE +RKL ID IR+ R D K++ QR A+ Sbjct: 399 INNNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQRGTAM 458 Query: 435 YFIDRLALTAXNEKDDEQ 488 Y ID AL A NEK +++ Sbjct: 459 YLIDVFALRAGNEKGEDE 476 >SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 782 Score = 25.4 bits (53), Expect = 4.8 Identities = 11/25 (44%), Positives = 14/25 (56%) Frame = +1 Query: 187 SGHKWREVRHDNTVTWLASWTEKFS 261 +G KW + H NT +L T KFS Sbjct: 529 TGEKWTMLHHINTTDYLNYETGKFS 553 >SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 351 Score = 25.0 bits (52), Expect = 6.3 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = +1 Query: 217 DNTVTWLASWTEKFSS 264 DN TWL + EKF+S Sbjct: 260 DNKCTWLVCYAEKFAS 275 >SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 25.0 bits (52), Expect = 6.3 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 246 DREVQQQAKYVMLNPSSKLKGEKDWQKYE 332 +REV K V+LNPS + E D +E Sbjct: 193 EREVIDGKKQVILNPSDSQRSEMDTFTFE 221 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 25.0 bits (52), Expect = 6.3 Identities = 11/37 (29%), Positives = 16/37 (43%) Frame = +3 Query: 318 WQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAV 428 W +E A C+ + N DWK +E + Q V Sbjct: 1822 WADFENAAD--SCVRHLENNEETDWKFEEWRQMQDVV 1856 >SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 520 Score = 25.0 bits (52), Expect = 6.3 Identities = 14/38 (36%), Positives = 17/38 (44%), Gaps = 3/38 (7%) Frame = -3 Query: 236 NHVTVLSCLTSLHLCPEG---GFGILLSFEQLIKTSSG 132 NH+ S + LH CP G G FE +I SG Sbjct: 345 NHLLPSSSVVDLHYCPYEDILGIGHAKGFESIIVPGSG 382 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 24.6 bits (51), Expect = 8.4 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +3 Query: 225 SYMVSLLDREVQQQAKYVMLNPSSKL 302 S +++L DRE AK +ML+ S+L Sbjct: 279 SRLLALADREADNNAKLIMLDRISQL 304 >SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subunit Pmh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 318 Score = 24.6 bits (51), Expect = 8.4 Identities = 15/31 (48%), Positives = 18/31 (58%) Frame = +3 Query: 237 SLLDREVQQQAKYVMLNPSSKLKGEKDWQKY 329 S+L +VQQ A+ P S L GEKD KY Sbjct: 233 SILLSDVQQVAEDT--TPFSPLAGEKDGSKY 261 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 24.6 bits (51), Expect = 8.4 Identities = 8/36 (22%), Positives = 20/36 (55%) Frame = +3 Query: 222 YSYMVSLLDREVQQQAKYVMLNPSSKLKGEKDWQKY 329 Y Y + D V ++++M+ ++L+ + DW ++ Sbjct: 971 YEYRKASSDMRVLMDSQFLMMKTFARLQAKGDWYEW 1006 >SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 24.6 bits (51), Expect = 8.4 Identities = 16/37 (43%), Positives = 18/37 (48%) Frame = -3 Query: 182 GFGILLSFEQLIKTSSGITRLFNFPIFGCSPRPLNNP 72 GF ILLSF I T SG F+ CS +N P Sbjct: 265 GFAILLSFNSPIWTMSGYDAPFHLS-EECSNASVNAP 300 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,850,234 Number of Sequences: 5004 Number of extensions: 34494 Number of successful extensions: 107 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 107 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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