Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0641.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g55310.1 68418.m06893 DNA topoisomerase I, putative similar t... 127 4e-30
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 126 6e-30
At1g04390.1 68414.m00429 expressed protein 29 1.7
At4g01270.1 68417.m00168 zinc finger (C3HC4-type RING finger) fa... 29 2.3
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 29 2.3
At1g74250.1 68414.m08599 DNAJ heat shock N-terminal domain-conta... 28 4.0
At1g56540.1 68414.m06502 disease resistance protein (TIR-NBS-LRR... 28 4.0
>At5g55310.1 68418.m06893 DNA topoisomerase I, putative similar to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 917
Score = 127 bits (307), Expect = 4e-30
Identities = 52/90 (57%), Positives = 72/90 (80%), Gaps = 1/90 (1%)
Frame = +1
Query: 1 QKEYGFCTIDGHKEKIGNFRIEPPGLFRGRGEHPKMGKLKRRVMPEDVLINCSKDSKIPK 180
+++Y + +DG +E+IGNFR+EPPGLFRGRGEHPKMGKLK+R+ P D+ IN K++ IP+
Sbjct: 483 EEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPE 542
Query: 181 -PPSGHKWREVRHDNTVTWLASWTEKFSSK 267
P G +W+EV+HDNTVTWLA W++ + K
Sbjct: 543 CPIPGERWKEVKHDNTVTWLAFWSDPINPK 572
Score = 76.6 bits (180), Expect = 9e-15
Identities = 39/76 (51%), Positives = 49/76 (64%)
Frame = +3
Query: 261 QQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAVALYF 440
++ KYV L SS LKG+ D +KYE ARKLH I IR D+ K++ Q AVA Y
Sbjct: 572 KEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYL 631
Query: 441 IDRLALTAXNEKDDEQ 488
ID+LAL A NEKDD++
Sbjct: 632 IDKLALRAGNEKDDDE 647
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 126 bits (305), Expect = 6e-30
Identities = 52/90 (57%), Positives = 71/90 (78%), Gaps = 1/90 (1%)
Frame = +1
Query: 1 QKEYGFCTIDGHKEKIGNFRIEPPGLFRGRGEHPKMGKLKRRVMPEDVLINCSKDSKIPK 180
+++Y + +DG KEKIGNFR+EPPGLFRGRGEHPKMGKLK+R+ P ++ +N K + IP+
Sbjct: 485 EEKYMWAVVDGVKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPE 544
Query: 181 -PPSGHKWREVRHDNTVTWLASWTEKFSSK 267
P +G +W+EV+HDNTVTWLA W + + K
Sbjct: 545 CPIAGERWKEVKHDNTVTWLAFWADPINPK 574
Score = 72.9 bits (171), Expect = 1e-13
Identities = 37/76 (48%), Positives = 49/76 (64%)
Frame = +3
Query: 261 QQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENXRADWKAKEMQVXQRAVALYF 440
++ KYV L S LKG D +KYE AR L ID IR ++ AK++++ Q AVA Y
Sbjct: 574 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 633
Query: 441 IDRLALTAXNEKDDEQ 488
ID+LAL A NEKDD++
Sbjct: 634 IDKLALRAGNEKDDDE 649
>At1g04390.1 68414.m00429 expressed protein
Length = 849
Score = 29.1 bits (62), Expect = 1.7
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +1
Query: 145 LINCSKDSKIPKPPSGHKWREVRHDNTVTWLASWTEKFS 261
L++ ++PKPPSG KW + + + L ++ E +S
Sbjct: 723 LVSWFYSDELPKPPSGCKWNNMDTEAKLDELQAYVEIYS 761
>At4g01270.1 68417.m00168 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 506
Score = 28.7 bits (61), Expect = 2.3
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = +3
Query: 240 LLDREVQQQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENXRADWKA-KEMQVX 416
LL EV++ V N +S L+ +K + E + KLH+C ++++E+ W+A +E+
Sbjct: 97 LLRGEVKRLEGKVQ-NLTSALEAKKK-ENVEVSDKLHQCNEQLKEDKVKRWEALQEISTT 154
Query: 417 QRAVAL 434
Q + L
Sbjct: 155 QHLLKL 160
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 28.7 bits (61), Expect = 2.3
Identities = 15/29 (51%), Positives = 17/29 (58%)
Frame = -3
Query: 257 NFSVQEANHVTVLSCLTSLHLCPEGGFGI 171
N VQEA +T SC+T H PE G GI
Sbjct: 326 NVQVQEAYGLTEHSCITLTHGDPEKGQGI 354
>At1g74250.1 68414.m08599 DNAJ heat shock N-terminal
domain-containing protein contains Pfam domains PF00226:
DnaJ domain and PF00096: Zinc finger, C2H2 type
Length = 630
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +3
Query: 291 SSKLKGEKDWQKYETARKLHKCIDKIREN 377
S K K EK W+ +E ++K + + ++RE+
Sbjct: 314 SKKFKSEKQWKNHEQSKKHKEKVAELRES 342
>At1g56540.1 68414.m06502 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1096
Score = 27.9 bits (59), Expect = 4.0
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +1
Query: 145 LINCSKDSKIPKPPSGHKWREVRH 216
L NC K + +PK PS KW H
Sbjct: 792 LSNCKKLTSLPKLPSSLKWLRASH 815
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,698,441
Number of Sequences: 28952
Number of extensions: 184300
Number of successful extensions: 506
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 504
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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