BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0629.Seq
(419 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g63550.1 68418.m07976 expressed protein 32 0.14
At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong sim... 29 0.97
At1g29470.1 68414.m03605 dehydration-responsive protein-related ... 29 1.7
At1g19880.1 68414.m02493 regulator of chromosome condensation (R... 29 1.7
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 28 2.2
At3g28770.1 68416.m03591 expressed protein 27 3.9
At2g20280.1 68415.m02369 zinc finger (CCCH-type) family protein ... 27 3.9
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 27 5.2
At1g56660.1 68414.m06516 expressed protein 27 5.2
At1g28420.1 68414.m03494 homeobox transcription factor, putative... 27 5.2
At1g15340.1 68414.m01835 methyl-CpG-binding domain-containing pr... 27 5.2
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf... 27 6.8
At5g61150.2 68418.m07672 leo1-like family protein weak similarit... 26 9.0
At5g22320.1 68418.m02604 leucine-rich repeat family protein cont... 26 9.0
At3g48120.1 68416.m05248 expressed protein 26 9.0
At1g13350.1 68414.m01550 protein kinase family protein contains ... 26 9.0
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 26 9.0
At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR... 26 9.0
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 26 9.0
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 32.3 bits (70), Expect = 0.14
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +3
Query: 180 DDSDKNRGKDTDDKYSETGTNKSSERRQASVMARRAASQSKGKNPNPT 323
++SD + DTDD+ E K S+++ +S +S SKGK+ P+
Sbjct: 315 EESDHEK-TDTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPS 361
>At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong
similarity to RNA helicase RH26 [Arabidopsis thaliana]
GI:3776025; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain; identical to cDNA DEAD box RNA helicase, RH26
GI:3776024
Length = 850
Score = 29.5 bits (63), Expect = 0.97
Identities = 16/49 (32%), Positives = 30/49 (61%)
Frame = +2
Query: 257 KTGKRDGSKSGVTVEREKSESNKKSREFENKEAESSTYRDKNRSXNSGS 403
+ G R+GS++G V+ + SES+ + R N ++ SS +++ +SGS
Sbjct: 177 RLGDREGSRNG-RVQGKSSESSFRGRSDRNVDSGSSFRGRSDKNVDSGS 224
>At1g29470.1 68414.m03605 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 770
Score = 28.7 bits (61), Expect = 1.7
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 245 IIRTKTGKRDGSKSGVTVEREKSESNKKSREFENKEAE 358
+ T K D KSG EK+ES ++ +EF++K +
Sbjct: 89 VTETNEEKTDPEKSGEENSGEKTESAEERKEFDDKNGD 126
>At1g19880.1 68414.m02493 regulator of chromosome condensation
(RCC1) family protein low similarity to UVB-resistance
protein UVR8 [Arabidopsis thaliana] GI:5478530; contains
Pfam profile PF00415: Regulator of chromosome
condensation (RCC1)
Length = 538
Score = 28.7 bits (61), Expect = 1.7
Identities = 14/45 (31%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +3
Query: 180 DDSDKNR---GKDTDDKYSETGTNKSSERRQASVMARRAASQSKG 305
D+SDK + G D D YSE G + +++ A R ++ +G
Sbjct: 471 DNSDKEKEVQGSDADSDYSEDGEEANGKKQSARGRGRGRGARGRG 515
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 28.3 bits (60), Expect = 2.2
Identities = 16/59 (27%), Positives = 27/59 (45%), Gaps = 4/59 (6%)
Frame = +2
Query: 251 RTKTGKRDGSKSGVTVEREK----SESNKKSREFENKEAESSTYRDKNRSXNSGSEPHE 415
R + + DG + E+E+ + ++SRE N+E++ RD R G E E
Sbjct: 172 RERREREDGERDRREREKERGSRRNRERERSREVGNEESDDDVKRDLKRRRKEGGERKE 230
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 27.5 bits (58), Expect = 3.9
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = +2
Query: 266 KRDGSKSGVTVEREKSESNKKSREFENKEAESSTYRDKN 382
K DGS +E ++ + + ++ ENKE+++ + DK+
Sbjct: 687 KNDGSSEKGEEGKENNKDSMEDKKLENKESQTDSKDDKS 725
>At2g20280.1 68415.m02369 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 371
Score = 27.5 bits (58), Expect = 3.9
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +2
Query: 299 EREKSESNKKSREFENKEAESSTYRDKNRSXNSGSEPHE 415
ERE+ E+ +K++ NKEAE+ T + + S E +E
Sbjct: 296 EREQEETEQKAK---NKEAEAGTSKSSGDAEQSSKEVNE 331
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 27.1 bits (57), Expect = 5.2
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +2
Query: 296 VEREKSESNKKSREFENKEAESSTYRDKNRS 388
+E+EK + +K +E + KE ++ +DK S
Sbjct: 102 LEKEKKDKERKEKERKEKERKAKEKKDKEES 132
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 27.1 bits (57), Expect = 5.2
Identities = 16/52 (30%), Positives = 23/52 (44%), Gaps = 1/52 (1%)
Frame = +2
Query: 263 GKRDGSKSGVTVEREKSESNK-KSREFENKEAESSTYRDKNRSXNSGSEPHE 415
G + +S V VE + E K K ++ E E E + KN+ S P E
Sbjct: 97 GDLEVKESDVKVEEHEKEHKKGKEKKHEELEEEKEGKKKKNKKEKDESGPEE 148
>At1g28420.1 68414.m03494 homeobox transcription factor, putative
similar to homeobox transcription factor Hox7 GI:19486
[Lycopersicon peruvianum]
Length = 1703
Score = 27.1 bits (57), Expect = 5.2
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +2
Query: 248 IRTKTGKRDGSKSGVTVEREKSESNKKSREFE-NKEAESSTYRDKNRSXNSGSEPHE 415
+R + + D S + V R + KS E E ++E E T+RD N +G P E
Sbjct: 1535 VRKRPERIDEDNSHL-VNRMANIVRPKSEEVEEDEEEEEQTFRDINEDWAAGETPRE 1590
>At1g15340.1 68414.m01835 methyl-CpG-binding domain-containing
protein contains Pfam profile PF01429: Methyl-CpG
binding domain
Length = 384
Score = 27.1 bits (57), Expect = 5.2
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = +2
Query: 266 KRDGSKSGVT--VEREKSESNKKSREFENKEAESSTYRDKNRS 388
K + K G T E EK + +K+ E ENKEAE RDK S
Sbjct: 175 KTEAGKEGQTEIAEAEKEKEGEKA-EAENKEAE--VVRDKKES 214
>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 883
Score = 26.6 bits (56), Expect = 6.8
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 180 DDSDKNRGKDTDDKYSETGTNKSSERRQAS 269
+DSDKN K+ +D + N S +++ S
Sbjct: 305 EDSDKNTSKENEDSGNSNKDNASKSKKKGS 334
>At5g61150.2 68418.m07672 leo1-like family protein weak similarity
to SP|P38439 LEO1 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04004: Leo1-like protein;
supporting cDNA gi|21929714|gb|AF490422.1|
Length = 623
Score = 26.2 bits (55), Expect = 9.0
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +3
Query: 192 KNRGKDTDDKYSETGTNKSSERRQASVMARRAASQSKG 305
+ RGKD++D+Y E ER +++ + + +G
Sbjct: 542 RGRGKDSEDEYEEDAEEDEEERGKSNRYSDEDEEEEEG 579
>At5g22320.1 68418.m02604 leucine-rich repeat family protein
contains leucine rich repeat (LRR) domains, Pfam:PF00560
Length = 452
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +3
Query: 207 DTDDKYSETGTNKSSERRQASVMARRAASQSKGKN 311
DTDD+ + NKS+E Q+ +R S + K+
Sbjct: 274 DTDDETFDAYHNKSAEEEQSKEDRKRKKSSKRNKS 308
>At3g48120.1 68416.m05248 expressed protein
Length = 328
Score = 26.2 bits (55), Expect = 9.0
Identities = 14/54 (25%), Positives = 25/54 (46%)
Frame = +2
Query: 227 RDRNQ*IIRTKTGKRDGSKSGVTVEREKSESNKKSREFENKEAESSTYRDKNRS 388
R+R++ + R + V+RE+ S +S E N+ + + R NRS
Sbjct: 103 RERDERDKSHRRRSRSSERRSSYVDRERRRSRSRSAERRNRYGDRESRRRSNRS 156
>At1g13350.1 68414.m01550 protein kinase family protein contains
protein kinase domain, Pfam:PF00069 (likely that this
cDNA contains a single unspliced intron. Putative intron
removed in this gene model.)
Length = 761
Score = 26.2 bits (55), Expect = 9.0
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +2
Query: 302 REKSESNKKSREFEN-KEAESSTYRDKNRSXNSGSEPHE 415
R+K+ + + R EN +E S RDK R S E H+
Sbjct: 156 RDKARYSSRERGRENERERRSEKDRDKGREFQSDREKHK 194
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 26.2 bits (55), Expect = 9.0
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = +3
Query: 195 NRGKDTDDKYSETGTNKSSERRQASVMARRAASQSKGKNPNPTR 326
+R + S + KSS R A +R +SK ++P+P R
Sbjct: 223 SRSRSRSRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRR 266
>At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 285
Score = 26.2 bits (55), Expect = 9.0
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = +3
Query: 195 NRGKDTDDKYSETGTNKSSERRQASVMARRAASQSKGKNPNPTR 326
+R + S + KSS R A +R +SK ++P+P R
Sbjct: 223 SRSRSRSRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRR 266
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 26.2 bits (55), Expect = 9.0
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = +3
Query: 195 NRGKDTDDKYSETGTNKSSERRQASVMARRAASQSKGKNPNPTR 326
+R + S + KSS R A +R +SK ++P+P R
Sbjct: 223 SRSRSRSRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRR 266
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,716,215
Number of Sequences: 28952
Number of extensions: 86884
Number of successful extensions: 485
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 448
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 485
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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