Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0628.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 45 6e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 44 1e-05
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 1.4
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 27 1.8
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 27 2.4
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding... 26 4.2
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 4.2
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 5.6
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 7.3
SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual 25 7.3
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 25 7.3
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 7.3
SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit S... 25 9.7
SPAC4D7.06c |||siroheme synthase |Schizosaccharomyces pombe|chr ... 25 9.7
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 9.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 63/100 (63%), Positives = 69/100 (69%), Gaps = 2/100 (2%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA T+ +KS +
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS-VEMHHES 296
Query: 428 LSKKLYLETIVGFNVKNVSRQGIASWXXAGDFKNKPT*GC 547
L L + VGFNVKNVS + I GD KN P GC
Sbjct: 297 LDAGLPGDN-VGFNVKNVSVKDIRRGNVCGDSKNDPPMGC 335
Score = 97.5 bits (232), Expect = 1e-21
Identities = 39/65 (60%), Positives = 51/65 (78%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 200
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 201 KADGK 215
GK
Sbjct: 220 VVKGK 224
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 63/100 (63%), Positives = 69/100 (69%), Gaps = 2/100 (2%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA T+ +KS +
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS-VEMHHES 296
Query: 428 LSKKLYLETIVGFNVKNVSRQGIASWXXAGDFKNKPT*GC 547
L L + VGFNVKNVS + I GD KN P GC
Sbjct: 297 LDAGLPGDN-VGFNVKNVSVKDIRRGNVCGDSKNDPPMGC 335
Score = 97.5 bits (232), Expect = 1e-21
Identities = 39/65 (60%), Positives = 51/65 (78%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 200
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 201 KADGK 215
GK
Sbjct: 220 VVKGK 224
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 63/100 (63%), Positives = 69/100 (69%), Gaps = 2/100 (2%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA T+ +KS +
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS-VEMHHES 296
Query: 428 LSKKLYLETIVGFNVKNVSRQGIASWXXAGDFKNKPT*GC 547
L L + VGFNVKNVS + I GD KN P GC
Sbjct: 297 LDAGLPGDN-VGFNVKNVSVKDIRRGNVCGDSKNDPPMGC 335
Score = 97.5 bits (232), Expect = 1e-21
Identities = 39/65 (60%), Positives = 51/65 (78%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 200
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 201 KADGK 215
GK
Sbjct: 220 VVKGK 224
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 45.2 bits (102), Expect = 6e-06
Identities = 24/55 (43%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKG 176
+SE RF+EIK VS + IK +G+ + V FVPIS G N++ + S W+KG
Sbjct: 330 WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG 384
Score = 29.5 bits (63), Expect = 0.34
Identities = 16/31 (51%), Positives = 19/31 (61%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 346
+P KPLRL + DVY+ TV GRVE G
Sbjct: 399 KPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 44.0 bits (99), Expect = 1e-05
Identities = 22/52 (42%), Positives = 29/52 (55%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNL 409
R TD P + ++DV+ I G GTV GRVE G LK G + S LK+ +
Sbjct: 247 RKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTV 298
Score = 27.1 bits (57), Expect = 1.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 385 NITTEVKSVEMHHEALQEAVPGDNC 459
++ T V +EM + L AV GDNC
Sbjct: 293 HLKTTVTGIEMFKKQLDAAVAGDNC 317
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.5 bits (58), Expect = 1.4
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +1
Query: 22 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 192
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 193 KKA 201
++
Sbjct: 205 SQS 207
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 27.1 bits (57), Expect = 1.8
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = -1
Query: 482 TRSLR*NLQLSPGTASWRASWCISTDLTSVVMLRGQRQQWYLVST 348
T L N PGT W SW L + V R + ++YL +T
Sbjct: 93 TWDLAANTTYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPCC1235.05c |fft2||fun thirty related protein
Fft2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1284
Score = 26.6 bits (56), Expect = 2.4
Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +1
Query: 385 NITTEVKS--VEMHHEALQEAVPGDNCRFQRKERVPS 489
NI TE+KS VE ++ L+E + G+N + VPS
Sbjct: 1155 NIKTEMKSETVEGDNKELRETMKGENVQTDSNAAVPS 1191
>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 279
Score = 25.8 bits (54), Expect = 4.2
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = -3
Query: 471 TLKPTIVSRYSFLESFVVHLHRFDFSSDVAGAKTTMVPGFNT 346
T+K + FLE+ VHLH ++ V GA PG+ T
Sbjct: 149 TIKDHNEVHFHFLEAIAVHLHFTQKANAVNGAN---APGYGT 187
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 4.2
Identities = 10/31 (32%), Positives = 14/31 (45%)
Frame = -1
Query: 278 DAGACQWGGRWQDGIESFNEAFSVSFAFFTL 186
D G C G G F++ +F FFT+
Sbjct: 287 DEGQCNPWGNSASGTREFSKVIGAAFTFFTI 317
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 5.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 351 NTPVSTLPTGTVPIPPIL 298
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.0 bits (52), Expect = 7.3
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +3
Query: 51 KEVS---SYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 176
KEVS SYI +P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
>SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.0 bits (52), Expect = 7.3
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -3
Query: 297 YTSCRGRRRGLSVGRQVAGWHRELQ 223
Y++C+G+R+ L +G ELQ
Sbjct: 123 YSTCQGKRKALLIGINYLNTQNELQ 147
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 25.0 bits (52), Expect = 7.3
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -1
Query: 389 MLRGQRQQWYLVSTHQFQLCRR 324
M+ G +Q W L+ TH C R
Sbjct: 697 MIHGPQQHWSLMPTHALMSCVR 718
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 7.3
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 118 LDGTETTCWSLQPKCLGSRDGRWSVKKAKLTENASLK 228
LD + W++QP C + DG A L++NA LK
Sbjct: 149 LDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit
S28|Schizosaccharomyces pombe|chr 2|||Manual
Length = 288
Score = 24.6 bits (51), Expect = 9.7
Identities = 12/34 (35%), Positives = 16/34 (47%), Gaps = 2/34 (5%)
Frame = -1
Query: 389 MLRGQRQQWYLVSTHQFQLC--RRARYQYHRFCI 294
+ G Q W L ST+ Q C + Q+H CI
Sbjct: 3 LFEGAFQPWKLASTNLMQQCLLLNKKSQFHTTCI 36
>SPAC4D7.06c |||siroheme synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 264
Score = 24.6 bits (51), Expect = 9.7
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +2
Query: 302 IGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCTTK 427
+GG G V GR+ + ++V +P L K WR K
Sbjct: 28 VGG-GVVAAGRILHVLNADAHVIVVSPKAGLCKEVAWRIQEK 68
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 24.6 bits (51), Expect = 9.7
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +3
Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 170
D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 108 DLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,546,729
Number of Sequences: 5004
Number of extensions: 54651
Number of successful extensions: 191
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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