BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0628.Seq (548 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 151 6e-39 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 151 6e-39 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 151 6e-39 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 139 1e-35 EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 41 1e-05 AY703685-1|AAU12681.1| 200|Apis mellifera abdominal-A protein. 24 1.2 DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. 23 2.0 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 6.2 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 6.2 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 151 bits (365), Expect = 6e-39 Identities = 66/72 (91%), Positives = 68/72 (94%) Frame = +3 Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQ 182 DSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW Sbjct: 83 DSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWT 142 Query: 183 VERKEGKADGKC 218 VERKEGK +GKC Sbjct: 143 VERKEGKVEGKC 154 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 151 bits (365), Expect = 6e-39 Identities = 66/72 (91%), Positives = 68/72 (94%) Frame = +3 Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQ 182 DSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW Sbjct: 99 DSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWT 158 Query: 183 VERKEGKADGKC 218 VERKEGK +GKC Sbjct: 159 VERKEGKVEGKC 170 Score = 101 bits (242), Expect = 5e-24 Identities = 54/92 (58%), Positives = 61/92 (66%) Frame = +2 Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCTTKLS 433 RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA + + Sbjct: 183 RPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEAL 242 Query: 434 KKLYLETIVGFNVKNVSRQGIASWXXAGDFKN 529 ++ VGFNVKNVS + + AGD KN Sbjct: 243 QEAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 274 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 151 bits (365), Expect = 6e-39 Identities = 66/72 (91%), Positives = 68/72 (94%) Frame = +3 Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQ 182 DSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW Sbjct: 156 DSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWT 215 Query: 183 VERKEGKADGKC 218 VERKEGK +GKC Sbjct: 216 VERKEGKVEGKC 227 Score = 104 bits (250), Expect = 5e-25 Identities = 56/97 (57%), Positives = 63/97 (64%) Frame = +2 Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCTTKLS 433 RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA + + Sbjct: 240 RPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEAL 299 Query: 434 KKLYLETIVGFNVKNVSRQGIASWXXAGDFKNKPT*G 544 ++ VGFNVKNVS + + AGD KN P G Sbjct: 300 QEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKG 336 Score = 31.5 bits (68), Expect = 0.006 Identities = 15/21 (71%), Positives = 15/21 (71%) Frame = +3 Query: 486 VKELRRG*XLVTSKTNPPKGA 548 VKELRRG SK NPPKGA Sbjct: 317 VKELRRGYVAGDSKNNPPKGA 337 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 139 bits (337), Expect = 1e-35 Identities = 60/71 (84%), Positives = 65/71 (91%) Frame = +3 Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQ 182 D T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+ Sbjct: 156 DMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWK 215 Query: 183 VERKEGKADGK 215 VERK+G ADGK Sbjct: 216 VERKDGNADGK 226 Score = 103 bits (248), Expect = 9e-25 Identities = 54/97 (55%), Positives = 63/97 (64%) Frame = +2 Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCTTKLS 433 RPTDK LRLPLQDVYKIGGIGTVPVGRVETG+LKPG +V FAPA + + Sbjct: 240 RPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEAL 299 Query: 434 KKLYLETIVGFNVKNVSRQGIASWXXAGDFKNKPT*G 544 + VGFNVKN+S + + AGD KN+P G Sbjct: 300 TEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRG 336 Score = 27.9 bits (59), Expect = 0.072 Identities = 13/21 (61%), Positives = 14/21 (66%) Frame = +3 Query: 486 VKELRRG*XLVTSKTNPPKGA 548 VKELRRG SK PP+GA Sbjct: 317 VKELRRGYVAGDSKNQPPRGA 337 >EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-alpha protein. Length = 119 Score = 40.7 bits (91), Expect = 1e-05 Identities = 18/24 (75%), Positives = 19/24 (79%) Frame = +1 Query: 403 KSVEMHHEALQEAVPGDNCRFQRK 474 KSVEMHHEAL EA+PGDN F K Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVK 24 Score = 32.3 bits (70), Expect = 0.003 Identities = 15/29 (51%), Positives = 19/29 (65%) Frame = +2 Query: 458 VGFNVKNVSRQGIASWXXAGDFKNKPT*G 544 VGFNVKN+S + + AGD KN+P G Sbjct: 19 VGFNVKNISVKELRRGYVAGDSKNQPPRG 47 Score = 27.9 bits (59), Expect = 0.072 Identities = 13/21 (61%), Positives = 14/21 (66%) Frame = +3 Query: 486 VKELRRG*XLVTSKTNPPKGA 548 VKELRRG SK PP+GA Sbjct: 28 VKELRRGYVAGDSKNQPPRGA 48 >AY703685-1|AAU12681.1| 200|Apis mellifera abdominal-A protein. Length = 200 Score = 23.8 bits (49), Expect = 1.2 Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 3/45 (6%) Frame = -3 Query: 459 TIVSRYSFLESFVVHLHRFDFSSDVA---GAKTTMVPGFNTPVST 334 T S Y ++ + H H + A GA ++MVPGF + ++ Sbjct: 84 TTSSMYPYVSAAAAHHHHQQQQAVAAAAFGATSSMVPGFGSTAAS 128 >DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. Length = 630 Score = 23.0 bits (47), Expect = 2.0 Identities = 12/41 (29%), Positives = 18/41 (43%) Frame = +2 Query: 368 VVFAPATSLLKSNLWRCTTKLSKKLYLETIVGFNVKNVSRQ 490 VV T L +W+ K S L+T+V + +V Q Sbjct: 276 VVIVSPTRELTIQIWQQIVKFSLNSILKTVVAYGGTSVMHQ 316 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 6.2 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -1 Query: 158 FG*RLQHVVSVPSRNGHESDSSWV 87 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 6.2 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +1 Query: 1 WIPLNHHTVSPDLRKSRRKY 60 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 172,940 Number of Sequences: 438 Number of extensions: 3839 Number of successful extensions: 25 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 19 length of database: 146,343 effective HSP length: 54 effective length of database: 122,691 effective search space used: 15704448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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