Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0628.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 95 2e-20
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 95 2e-20
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 95 2e-20
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 95 2e-20
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 46 1e-05
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 42 3e-04
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 39 0.002
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 35 0.041
At1g30900.1 68414.m03780 vacuolar sorting receptor, putative sim... 29 1.5
At2g17820.1 68415.m02064 histidine kinase 1 99% identical to GP:... 29 2.0
At2g39560.1 68415.m04853 expressed protein 29 2.7
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 3.6
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 3.6
At3g18370.1 68416.m02336 C2 domain-containing protein contains P... 28 3.6
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 4.7
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 6.2
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 6.2
At3g20020.1 68416.m02533 protein arginine N-methyltransferase fa... 27 6.2
At1g61370.1 68414.m06916 S-locus lectin protein kinase family pr... 27 6.2
At1g35610.1 68414.m04421 DC1 domain-containing protein contains ... 27 6.2
At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to... 27 8.3
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 95.5 bits (227), Expect = 2e-20
Identities = 54/103 (52%), Positives = 69/103 (66%), Gaps = 6/103 (5%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RP+DKPLRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAP T+ +KS +
Sbjct: 228 RPSDKPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKS------VE 281
Query: 428 LSKKLYLETI----VGFNVKNVSRQGIASWXXAGDFKNKPT*G 544
+ + LE + VGFNVKNV+ + + A + K+ P G
Sbjct: 282 MHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 87.8 bits (208), Expect = 4e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +3
Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 176
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.5 bits (227), Expect = 2e-20
Identities = 54/103 (52%), Positives = 69/103 (66%), Gaps = 6/103 (5%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RP+DKPLRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAP T+ +KS +
Sbjct: 228 RPSDKPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKS------VE 281
Query: 428 LSKKLYLETI----VGFNVKNVSRQGIASWXXAGDFKNKPT*G 544
+ + LE + VGFNVKNV+ + + A + K+ P G
Sbjct: 282 MHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 87.8 bits (208), Expect = 4e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +3
Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 176
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.5 bits (227), Expect = 2e-20
Identities = 54/103 (52%), Positives = 69/103 (66%), Gaps = 6/103 (5%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RP+DKPLRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAP T+ +KS +
Sbjct: 228 RPSDKPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKS------VE 281
Query: 428 LSKKLYLETI----VGFNVKNVSRQGIASWXXAGDFKNKPT*G 544
+ + LE + VGFNVKNV+ + + A + K+ P G
Sbjct: 282 MHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 87.8 bits (208), Expect = 4e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +3
Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 176
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.5 bits (227), Expect = 2e-20
Identities = 54/103 (52%), Positives = 69/103 (66%), Gaps = 6/103 (5%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA--TSLLKSNLWRCTTK 427
RP+DKPLRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAP T+ +KS +
Sbjct: 228 RPSDKPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKS------VE 281
Query: 428 LSKKLYLETI----VGFNVKNVSRQGIASWXXAGDFKNKPT*G 544
+ + LE + VGFNVKNV+ + + A + K+ P G
Sbjct: 282 MHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 87.8 bits (208), Expect = 4e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +3
Query: 3 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 176
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 46.4 bits (105), Expect = 1e-05
Identities = 20/40 (50%), Positives = 26/40 (65%)
Frame = +2
Query: 242 PATCRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 361
P R DKP +P++DV+ I G GTV GR+E GV+K G
Sbjct: 258 PDPVRVLDKPFLMPIEDVFSIQGRGTVATGRIEQGVIKVG 297
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 41.9 bits (94), Expect = 3e-04
Identities = 27/78 (34%), Positives = 40/78 (51%), Gaps = 1/78 (1%)
Frame = +2
Query: 242 PATCRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCT 421
P R T+ P L ++DV+ I G GTV GRVE G +K G V L ++ + T
Sbjct: 278 PIPQRQTELPFLLAVEDVFSITGRGTVATGRVERGTVKVGETV---DLVGLRETRSYTVT 334
Query: 422 -TKLSKKLYLETIVGFNV 472
++ +K+ E + G NV
Sbjct: 335 GVEMFQKILDEALAGDNV 352
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 39.1 bits (87), Expect = 0.002
Identities = 20/55 (36%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--MPWFKG 176
+S+ R++EI++++ ++K GYN V F+PISG G NM + + PW+ G
Sbjct: 256 WSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPWWSG 310
Score = 33.9 bits (74), Expect = 0.072
Identities = 17/49 (34%), Positives = 29/49 (59%)
Frame = +2
Query: 254 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLK 400
R + P R+P+ D +K +GTV +G+VE+G ++ G +V P +K
Sbjct: 325 RDPNGPFRMPIIDKFK--DMGTVVMGKVESGSIREGDSLVVMPNKEQVK 371
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 34.7 bits (76), Expect = 0.041
Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +3
Query: 21 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--MPWFKG 176
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W++G
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At1g30900.1 68414.m03780 vacuolar sorting receptor, putative
similar to BP-80 vacuolar sorting receptor [Pisum
sativum] GI:1737222
Length = 631
Score = 29.5 bits (63), Expect = 1.5
Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 7/57 (12%)
Frame = +1
Query: 109 CPFLDGTE------TTCWSLQP-KCLGSRDGRWSVKKAKLTENASLKLSMPSCHLPP 258
CP ++G + T+C P +C ++ G WS K LT +A L C PP
Sbjct: 446 CPVVNGVQYKGDGYTSCEPYGPARCSINQGGCWSETKKGLTFSACSNLETSGCRCPP 502
>At2g17820.1 68415.m02064 histidine kinase 1 99% identical to
GP:4586626
Length = 1207
Score = 29.1 bits (62), Expect = 2.0
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +2
Query: 242 PATCRPTDKPLRLPLQDVYKIGGIGTVPVG 331
P T KPL++P D+ I GI VP G
Sbjct: 252 PVTGENLGKPLKIPPDDLINIAGISQVPDG 281
>At2g39560.1 68415.m04853 expressed protein
Length = 233
Score = 28.7 bits (61), Expect = 2.7
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -3
Query: 366 MVPGFNTPVSTLPTGTVPIPPILYTSCRGRRRGLS 262
+ P + TP ++ T P+ P+L SC GR+ +S
Sbjct: 144 ITPPYLTPRASPSLFTPPLTPLLMESCNGRKEEIS 178
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 3.6
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +2
Query: 77 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 178
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 3.6
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +3
Query: 24 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 113
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 815
Score = 28.3 bits (60), Expect = 3.6
Identities = 24/68 (35%), Positives = 28/68 (41%), Gaps = 4/68 (5%)
Frame = +1
Query: 49 RRKYPHTSRRLATTQLLSLS---CPFLDGTETTCWSLQPKCLGSR-DGRWSVKKAKLTEN 216
+R SR + QLL S CP L G TCWS + R D W +
Sbjct: 127 KRLRQRRSRLIENIQLLEFSLGSCPPLLGLHGTCWSKSGEQKIMRLDFNWDTTDLSILLQ 186
Query: 217 ASLKLSMP 240
A KLSMP
Sbjct: 187 A--KLSMP 192
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 4.7
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +3
Query: 30 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 140
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 6 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 119
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 6 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 119
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g20020.1 68416.m02533 protein arginine N-methyltransferase
family protein similar to SP|Q96LA8 Protein arginine
N-methyltransferase 6 (EC 2.1.1.-) {Homo sapiens}
Length = 435
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/52 (26%), Positives = 24/52 (46%)
Frame = -3
Query: 459 TIVSRYSFLESFVVHLHRFDFSSDVAGAKTTMVPGFNTPVSTLPTGTVPIPP 304
++ +RY F +H F F DV + P NT +++ +G+ I P
Sbjct: 300 SVTARYKFNSMMRAPMHGFAFWFDVEFSGPASSPAKNTSETSIASGSSSISP 351
>At1g61370.1 68414.m06916 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 814
Score = 27.5 bits (58), Expect = 6.2
Identities = 19/76 (25%), Positives = 35/76 (46%)
Frame = +2
Query: 293 VYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSLLKSNLWRCTTKLSKKLYLETIVGFNV 472
V+ IG T + +L+ G +V+ + + NLW L + LE+ V ++V
Sbjct: 108 VWSIGE--TFSSNELRAELLENGNLVLI---DGVSERNLWESFEHLGDTMLLESSVMYDV 162
Query: 473 KNVSRQGIASWXXAGD 520
N ++ ++SW D
Sbjct: 163 PNNKKRVLSSWKNPTD 178
>At1g35610.1 68414.m04421 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 612
Score = 27.5 bits (58), Expect = 6.2
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = -1
Query: 362 YLVSTHQFQLCRRARYQYHRFCIRLA 285
Y + +HQF C++ Y H C L+
Sbjct: 334 YPIVSHQFYHCKKCNYSLHEVCAGLS 359
>At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to
homeotic protein HAT 3.1 (GI:11994474) [Arabidopsis
thaliana]
Length = 723
Score = 27.1 bits (57), Expect = 8.3
Identities = 8/38 (21%), Positives = 20/38 (52%)
Frame = +3
Query: 27 EPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 140
+ + IKK++ ++ +I Y + + + GW G ++
Sbjct: 174 DDEYTRIKKKLRYFLNRINYEQSLIDAYSLEGWKGSSL 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,801,976
Number of Sequences: 28952
Number of extensions: 306222
Number of successful extensions: 1014
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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