BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0616.Seq (488 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 117 1e-27 SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 117 1e-27 SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 83 2e-17 SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyc... 30 0.22 SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 30 0.22 SPCC830.10 |||nucleoside triphosphatase |Schizosaccharomyces pom... 28 0.87 SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 28 0.87 SPBC21C3.04c |||mitochondrial ribosomal protein subunit L34|Schi... 27 1.5 SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 26 3.5 SPCC584.02 |cuf2||Cu metalloregulatory transcription factor Cuf2... 25 4.6 SPAC17A5.16 |||human down-regulated in multiple cancers-1 homolo... 25 6.1 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 6.1 SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein Did4... 25 8.1 SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces p... 25 8.1 >SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces pombe|chr 2|||Manual Length = 251 Score = 117 bits (281), Expect = 1e-27 Identities = 50/115 (43%), Positives = 77/115 (66%), Gaps = 1/115 (0%) Frame = -3 Query: 372 IAEPYIAWGYPNLKSVRELVXXTWIRQAEWTTYTNHFQQLVEKRLHKHNXICVEDLIHEI 193 + EPY+ +G PNLK+VREL+ + ++E L K++ + +EDLIHEI Sbjct: 137 VVEPYVTYGIPNLKTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSILSIEDLIHEI 196 Query: 192 FTVGEKFKYASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKINDLLXRMV 31 +TVG FK A+NF+WPF+L++P GGWR +K H+++GGD G R++ IN L+ +M+ Sbjct: 197 YTVGPNFKQAANFIWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSLVRKML 251 Score = 58.8 bits (136), Expect = 4e-10 Identities = 29/52 (55%), Positives = 33/52 (63%) Frame = -2 Query: 487 GINQVSXKVRKVQQLFRLRQINNGVFVRLNKATVNMLRYRRALHCLGIPQLK 332 GIN + K RK+ QL RL QINNGVFV+ NKAT ML+ GIP LK Sbjct: 99 GINNIPPKARKIMQLLRLIQINNGVFVKFNKATKEMLQVVEPYVTYGIPNLK 150 >SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 117 bits (281), Expect = 1e-27 Identities = 52/115 (45%), Positives = 76/115 (66%), Gaps = 1/115 (0%) Frame = -3 Query: 372 IAEPYIAWGYPNLKSVRELVXXTWIRQAEWTTYTNHFQQLVEKRLHKHNXICVEDLIHEI 193 + EPY+ +G PN K+VREL+ + ++E L K++ + VEDLIHEI Sbjct: 136 VVEPYVTYGIPNHKTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEI 195 Query: 192 FTVGEKFKYASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKINDLLXRMV 31 +TVG FK A+NFLWPFKL++P GGWR +K H+++GGD G R++ IN L+ +M+ Sbjct: 196 YTVGPNFKQAANFLWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGLVQKML 250 Score = 53.6 bits (123), Expect = 2e-08 Identities = 26/52 (50%), Positives = 31/52 (59%) Frame = -2 Query: 487 GINQVSXKVRKVQQLFRLRQINNGVFVRLNKATVNMLRYRRALHCLGIPQLK 332 GIN + K RK+ QL RL QINNG+FV+ NKA ML+ GIP K Sbjct: 98 GINNIPPKARKIMQLLRLLQINNGIFVKFNKAIKEMLQVVEPYVTYGIPNHK 149 >SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 83.4 bits (197), Expect = 2e-17 Identities = 43/115 (37%), Positives = 62/115 (53%), Gaps = 1/115 (0%) Frame = -3 Query: 372 IAEPYIAWGYPNLKSVRELVXXTWIRQAEWTTYTNHFQQLVEKRLHKHNXICVEDLIHEI 193 I EPY+ +G PNL SVREL+ + L+E+ L K++ I +ED+IHEI Sbjct: 135 IVEPYVMYGIPNLHSVRELIYKRGFGKINGQRIALSDNALIEEALGKYDVISIEDIIHEI 194 Query: 192 FTVGEKFKYASNFLWPFKLNNPTGG-WRKKTIHYVDGGDFGNREDKINDLLXRMV 31 + VG FK + FLWPF L KK H+ +G G ++IN+L+ + V Sbjct: 195 YNVGSHFKEVTKFLWPFTLTPVKHSLMEKKVKHFNEGRKAGYCGEEINELIKKQV 249 Score = 49.6 bits (113), Expect = 2e-07 Identities = 25/51 (49%), Positives = 30/51 (58%) Frame = -2 Query: 487 GINQVSXKVRKVQQLFRLRQINNGVFVRLNKATVNMLRYRRALHCLGIPQL 335 G+ + K+RKV +L RL +INN VFVR NKA MLR GIP L Sbjct: 97 GVKNMPPKIRKVLRLLRLSRINNAVFVRNNKAVAQMLRIVEPYVMYGIPNL 147 >SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyces pombe|chr 3|||Manual Length = 430 Score = 29.9 bits (64), Expect = 0.22 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = +2 Query: 110 LRQPPVGLFNLKGHRKLLAYLNFSPTVKISWMRSSTQXMLCLWSL 244 LR P L L+GH + L +SP + SST +C+W L Sbjct: 311 LRNPYQRLHTLEGHEDEVYGLEWSPHDEPILASSSTDRRVCIWDL 355 >SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 609 Score = 29.9 bits (64), Expect = 0.22 Identities = 14/43 (32%), Positives = 25/43 (58%) Frame = -2 Query: 457 KVQQLFRLRQINNGVFVRLNKATVNMLRYRRALHCLGIPQLKE 329 ++ L ++ G FV+L + TVN+++ R+ L LG+ L E Sbjct: 65 QLNHLVAENKLQKGAFVQLTQFTVNVMKERKILIVLGLNVLTE 107 >SPCC830.10 |||nucleoside triphosphatase |Schizosaccharomyces pombe|chr 3|||Manual Length = 188 Score = 27.9 bits (59), Expect = 0.87 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = +1 Query: 97 MNGLLAPTTSWIVQFEGPQEITRVLELFSNSE 192 MNGL P W + GP + R++ F E Sbjct: 75 MNGLPGPYVKWFLNSVGPDGLYRMVSAFDTKE 106 >SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 27.9 bits (59), Expect = 0.87 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +3 Query: 360 KARRYRSIFTVALFRRTNTPLFIWRSLNS 446 K R YRS F V L +NTP+ + +L S Sbjct: 280 KFRGYRSTFPVKLLYTSNTPIMLQSALTS 308 >SPBC21C3.04c |||mitochondrial ribosomal protein subunit L34|Schizosaccharomyces pombe|chr 2|||Manual Length = 108 Score = 27.1 bits (57), Expect = 1.5 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = -3 Query: 300 IRQAEWTTYTNHFQQLVEKRLHKHNXI 220 ++Q W TY N +Q KR KH + Sbjct: 57 MQQVRWKTYGNEYQPSNRKRKRKHGFL 83 >SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 747 Score = 25.8 bits (54), Expect = 3.5 Identities = 29/110 (26%), Positives = 54/110 (49%), Gaps = 7/110 (6%) Frame = +1 Query: 1 FFYNYKSIYLNHPXEKIVDLV----FAVTKVSPVDI-MNGLLAPTTSWI--VQFEGPQEI 159 F N I++ P +K ++L+ +A++ V +I N +L+ T+ I V + Sbjct: 386 FLPNLLEIWVQQPPDKRLELLELVQYALSNVDSDEIPSNVMLSVCTNLINEVASQNKYSS 445 Query: 160 TRVLELFSNSEDLMDEVLNTDXVVFMEPLLNKLLEVIGIRCPLSLANPRS 309 T + ++ +N E ++E + ++ + LLN + VIG L L NP S Sbjct: 446 TEISQITTNRE--VEE--ENEEILLV--LLNIISSVIGRNAELDLENPIS 489 >SPCC584.02 |cuf2||Cu metalloregulatory transcription factor Cuf2|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 25.4 bits (53), Expect = 4.6 Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 3/60 (5%) Frame = +1 Query: 130 IVQFEGPQEITRVLELFSNSEDLMDEVLNTDXVVFMEPLLNKL---LEVIGIRCPLSLAN 300 ++ F I L LFS +D+M+ LN + L L +++G R PLSL + Sbjct: 75 LLNFTQKNPILASLFLFSTDKDIMNSSLNPASQAYTFDLGRTLPISEDILGYRKPLSLTD 134 >SPAC17A5.16 |||human down-regulated in multiple cancers-1 homolog 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 25.0 bits (52), Expect = 6.1 Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 5/71 (7%) Frame = +1 Query: 88 VDIMNGLLAPTTSWIVQFEGPQEITRVLELFSN-----SEDLMDEVLNTDXVVFMEPLLN 252 ++I + L AP+ SW + E+ +L + S + + +E+ TD V + L N Sbjct: 763 LEIDSNLFAPSNSWFNSWHSRLELDSILAIISQFSLPVYKKMNEELSTTDEAVKL--LAN 820 Query: 253 KLLEVIGIRCP 285 +L + R P Sbjct: 821 SVLNDVHPRVP 831 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 25.0 bits (52), Expect = 6.1 Identities = 17/71 (23%), Positives = 33/71 (46%) Frame = +1 Query: 4 FYNYKSIYLNHPXEKIVDLVFAVTKVSPVDIMNGLLAPTTSWIVQFEGPQEITRVLELFS 183 F + ++ HP + + K+ + I++ L VQ + E+ ++ LF Sbjct: 1729 FSIFYEVFFQHPSTNVYANDEGI-KIGALQIISFFLKNVPEITVQHQ--TEMLKMCSLFG 1785 Query: 184 NSEDLMDEVLN 216 NSED+M + L+ Sbjct: 1786 NSEDVMIKQLS 1796 >SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein Did4|Schizosaccharomyces pombe|chr 1|||Manual Length = 210 Score = 24.6 bits (51), Expect = 8.1 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +1 Query: 136 QFEGPQEITRVLELFSNSEDLMDEVLNTDXVVFMEPLLNKLLEVIGI 276 QFE EI + ++ MD+ L D + L+NK+L+ IG+ Sbjct: 131 QFERENEIMEQRQ--EMIDENMDDALEEDDEEEADELVNKVLDEIGV 175 >SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 464 Score = 24.6 bits (51), Expect = 8.1 Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Frame = -3 Query: 237 HKH-NXICVEDLIHEIFTVGEKFKYASNFLWPFKLNNPTGGWRKKTIHYVD 88 H+H + +C + + VGE + F+ F +N T KKT+ +V+ Sbjct: 106 HQHYSRVCRNGIDINLIVVGESSLGKTTFVNSFLQSNDTNFRPKKTMDFVE 156 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,013,265 Number of Sequences: 5004 Number of extensions: 41744 Number of successful extensions: 146 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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