Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0614.Seq
(309 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015B4431 Cluster: PREDICTED: similar to calreticul... 53 1e-06
UniRef50_P27797 Cluster: Calreticulin precursor; n=144; Eukaryot... 44 9e-04
UniRef50_UPI0000F2BC3E Cluster: PREDICTED: hypothetical protein;... 43 0.002
UniRef50_UPI000155CE78 Cluster: PREDICTED: similar to Calreticul... 41 0.005
UniRef50_Q9XYF8 Cluster: Calreticulin; n=9; Eukaryota|Rep: Calre... 39 0.025
UniRef50_A2E9B3 Cluster: Calreticulin family protein; n=1; Trich... 37 0.10
UniRef50_Q96L12 Cluster: Calreticulin-3 precursor; n=15; Theria|... 36 0.23
UniRef50_Q5ENL5 Cluster: Calrectulin; n=1; Heterocapsa triquetra... 35 0.31
UniRef50_A7PRT0 Cluster: Chromosome chr14 scaffold_27, whole gen... 32 2.9
UniRef50_Q23858 Cluster: Calreticulin precursor; n=9; Eukaryota|... 32 2.9
UniRef50_O04153 Cluster: Calreticulin-3 precursor; n=78; Eukaryo... 32 2.9
UniRef50_Q4RWP9 Cluster: Chromosome 15 SCAF14981, whole genome s... 31 3.8
UniRef50_Q58H60 Cluster: YNR5; n=9; Oryza sativa|Rep: YNR5 - Ory... 31 3.8
UniRef50_UPI000049921C Cluster: calreticulin; n=1; Entamoeba his... 30 8.8
UniRef50_Q1KSI9 Cluster: Calreticulin; n=1; Phaseolus vulgaris|R... 30 8.8
UniRef50_A5BWC7 Cluster: Putative uncharacterized protein; n=1; ... 30 8.8
UniRef50_A2D8F5 Cluster: Putative uncharacterized protein; n=1; ... 30 8.8
>UniRef50_UPI00015B4431 Cluster: PREDICTED: similar to calreticulin,
partial; n=1; Nasonia vitripennis|Rep: PREDICTED:
similar to calreticulin, partial - Nasonia vitripennis
Length = 134
Score = 53.2 bits (122), Expect = 1e-06
Identities = 28/72 (38%), Positives = 36/72 (50%)
Frame = +2
Query: 92 DVFFEEKFPDDSWESNWVYSXAPXKRIWKV*IDCWKVLQRPTG*QRFENL*RC*VYALSR 271
+VFFEEKF DDSWE NW+YS + K + + P + + YA+SR
Sbjct: 19 EVFFEEKFIDDSWEHNWIYSEHQGQEFGKFTLSQGQFWNDPDNDKGIQTSQDARFYAVSR 78
Query: 272 XFKPXSXEGKPL 307
F P S E K L
Sbjct: 79 RFPPFSNENKSL 90
Score = 49.6 bits (113), Expect = 1e-05
Identities = 19/34 (55%), Positives = 28/34 (82%)
Frame = +3
Query: 153 EHPXKEFGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
EH +EFGKF L+ G+F++DP +DKG++T +DAR
Sbjct: 39 EHQGQEFGKFTLSQGQFWNDPDNDKGIQTSQDAR 72
>UniRef50_P27797 Cluster: Calreticulin precursor; n=144;
Eukaryota|Rep: Calreticulin precursor - Homo sapiens
(Human)
Length = 417
Score = 43.6 bits (98), Expect = 9e-04
Identities = 17/29 (58%), Positives = 24/29 (82%)
Frame = +3
Query: 168 EFGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
+FGKF L++GKF+ D + DKGL+T +DAR
Sbjct: 45 DFGKFVLSSGKFYGDEEKDKGLQTSQDAR 73
>UniRef50_UPI0000F2BC3E Cluster: PREDICTED: hypothetical protein;
n=1; Monodelphis domestica|Rep: PREDICTED: hypothetical
protein - Monodelphis domestica
Length = 549
Score = 42.7 bits (96), Expect = 0.002
Identities = 16/29 (55%), Positives = 24/29 (82%)
Frame = +3
Query: 168 EFGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
++GKFKLTAG F+ + + DKG++T EDA+
Sbjct: 159 DYGKFKLTAGMFYGNKEKDKGIQTTEDAK 187
>UniRef50_UPI000155CE78 Cluster: PREDICTED: similar to Calreticulin
3; n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar
to Calreticulin 3 - Ornithorhynchus anatinus
Length = 410
Score = 41.1 bits (92), Expect = 0.005
Identities = 16/28 (57%), Positives = 23/28 (82%)
Frame = +3
Query: 171 FGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
FGKFKL+AGKF+ D D+GL+T E+++
Sbjct: 50 FGKFKLSAGKFYGDAMRDRGLQTTENSK 77
Score = 31.9 bits (69), Expect = 2.9
Identities = 26/75 (34%), Positives = 33/75 (44%), Gaps = 4/75 (5%)
Frame = +2
Query: 95 VFFEEKFPD-DSWESNWVYSXAPXKRIWKV*IDCWKVL---QRPTG*QRFENL*RC*VYA 262
V+F+E+F D D W+ W+ S K + K R G Q EN YA
Sbjct: 25 VYFQEEFLDGDGWKKRWLQS-THDPNFGKFKLSAGKFYGDAMRDRGLQTTEN---SKFYA 80
Query: 263 LSRXFKPXSXEGKPL 307
+S FKP S GK L
Sbjct: 81 ISSRFKPFSNRGKSL 95
>UniRef50_Q9XYF8 Cluster: Calreticulin; n=9; Eukaryota|Rep:
Calreticulin - Trypanosoma cruzi
Length = 403
Score = 38.7 bits (86), Expect = 0.025
Identities = 15/29 (51%), Positives = 23/29 (79%)
Frame = +3
Query: 168 EFGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
+FGK +++AGKF++D + KGL+ EDAR
Sbjct: 43 DFGKVEISAGKFYADAEKRKGLRLTEDAR 71
>UniRef50_A2E9B3 Cluster: Calreticulin family protein; n=1;
Trichomonas vaginalis G3|Rep: Calreticulin family
protein - Trichomonas vaginalis G3
Length = 425
Score = 36.7 bits (81), Expect = 0.10
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +3
Query: 159 PXKEFGKFKLTAGKFFSDPQDDKGLKTXEDARS 257
P K FG+++LTAG DP +G+KT +D R+
Sbjct: 37 PGKPFGRWQLTAGSTPGDPAKQRGIKTLDDFRN 69
>UniRef50_Q96L12 Cluster: Calreticulin-3 precursor; n=15;
Theria|Rep: Calreticulin-3 precursor - Homo sapiens
(Human)
Length = 384
Score = 35.5 bits (78), Expect = 0.23
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = +3
Query: 171 FGKFKLTAGKFFSDPQDDKGLKTXEDAR 254
FG F+L++GKF+ + DKGL+T ++ R
Sbjct: 46 FGHFRLSSGKFYGHKEKDKGLQTTQNGR 73
>UniRef50_Q5ENL5 Cluster: Calrectulin; n=1; Heterocapsa
triquetra|Rep: Calrectulin - Heterocapsa triquetra
(Dinoflagellate)
Length = 241
Score = 35.1 bits (77), Expect = 0.31
Identities = 13/27 (48%), Positives = 22/27 (81%)
Frame = +3
Query: 174 GKFKLTAGKFFSDPQDDKGLKTXEDAR 254
GK+ AGK+F+D ++DKG++T ED++
Sbjct: 45 GKWVSAAGKWFADEKEDKGIQTGEDSK 71
>UniRef50_A7PRT0 Cluster: Chromosome chr14 scaffold_27, whole genome
shotgun sequence; n=9; Magnoliophyta|Rep: Chromosome
chr14 scaffold_27, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 438
Score = 31.9 bits (69), Expect = 2.9
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = +2
Query: 95 VFFEEKFPDDSWESNWVYS 151
VFFEE+F DD WE+ WV S
Sbjct: 28 VFFEERF-DDGWENKWVKS 45
Score = 30.3 bits (65), Expect = 8.8
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +3
Query: 174 GKFKLTAGKFFSDPQDDKGLKTXEDAR 254
G++ T+GK+ DP +DKG++T ED R
Sbjct: 55 GEWNYTSGKWHGDP-NDKGIQTSEDYR 80
>UniRef50_Q23858 Cluster: Calreticulin precursor; n=9;
Eukaryota|Rep: Calreticulin precursor - Dictyostelium
discoideum (Slime mold)
Length = 424
Score = 31.9 bits (69), Expect = 2.9
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +3
Query: 174 GKFKLTAGKFFSDPQDDKGLKTXEDAR 254
GK TAGK+F D ++ KG++T EDAR
Sbjct: 48 GKLVHTAGKWFGD-ENQKGIQTSEDAR 73
>UniRef50_O04153 Cluster: Calreticulin-3 precursor; n=78;
Eukaryota|Rep: Calreticulin-3 precursor - Arabidopsis
thaliana (Mouse-ear cress)
Length = 424
Score = 31.9 bits (69), Expect = 2.9
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +3
Query: 174 GKFKLTAGKFFSDPQDDKGLKTXEDAR 254
G FK TAGK+ DP D+KG++T DA+
Sbjct: 57 GTFKHTAGKWPGDP-DNKGIQTYNDAK 82
>UniRef50_Q4RWP9 Cluster: Chromosome 15 SCAF14981, whole genome
shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 15
SCAF14981, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 471
Score = 31.5 bits (68), Expect = 3.8
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +3
Query: 168 EFGKFKLTAGKFFSDPQDDKG 230
++G+ KLTAG F+ D + DKG
Sbjct: 47 DYGELKLTAGNFYGDAEKDKG 67
>UniRef50_Q58H60 Cluster: YNR5; n=9; Oryza sativa|Rep: YNR5 - Oryza
sativa subsp. japonica (Rice)
Length = 960
Score = 31.5 bits (68), Expect = 3.8
Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 6/39 (15%)
Frame = +3
Query: 138 IGCTVEH------PXKEFGKFKLTAGKFFSDPQDDKGLK 236
+GC++E P K G+FK+TAGKF + PQ +GL+
Sbjct: 724 LGCSMEFLRSLSDPPKFLGEFKVTAGKFANVPQWIEGLE 762
>UniRef50_UPI000049921C Cluster: calreticulin; n=1; Entamoeba
histolytica HM-1:IMSS|Rep: calreticulin - Entamoeba
histolytica HM-1:IMSS
Length = 139
Score = 30.3 bits (65), Expect = 8.8
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +3
Query: 165 KEFGKFKLTAGKFFSDPQDDKGLKTXED 248
K G FK+ +GK++ D ++KGL+T ED
Sbjct: 35 KALGPFKIVSGKWYGD-ANNKGLQTSED 61
>UniRef50_Q1KSI9 Cluster: Calreticulin; n=1; Phaseolus vulgaris|Rep:
Calreticulin - Phaseolus vulgaris (Kidney bean) (French
bean)
Length = 111
Score = 30.3 bits (65), Expect = 8.8
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +2
Query: 95 VFFEEKFPDDSWESNWVYS 151
VFFEE+F +D WE+ WV S
Sbjct: 26 VFFEERF-EDGWENRWVKS 43
>UniRef50_A5BWC7 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 172
Score = 30.3 bits (65), Expect = 8.8
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +3
Query: 174 GKFKLTAGKFFSDPQDDKGLKTXEDAR 254
G++ T+GK+ DP +DKG++T ED R
Sbjct: 102 GEWNCTSGKWHGDP-NDKGIQTNEDYR 127
>UniRef50_A2D8F5 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 111
Score = 30.3 bits (65), Expect = 8.8
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = -3
Query: 253 LASSXVFKPLSSCGSLKNFPAVNLNFPNSFXGCSTVHPIAFPRI 122
L S FK S ++ P+ + +P+SF GC+++ + PR+
Sbjct: 59 LCSEEAFKDCISLETI-TLPSTRIIYPSSFKGCTSLKSFSIPRL 101
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 242,150,208
Number of Sequences: 1657284
Number of extensions: 3495988
Number of successful extensions: 7155
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 6908
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7149
length of database: 575,637,011
effective HSP length: 79
effective length of database: 444,711,575
effective search space used: 10228366225
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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