Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0613.Seq
(489 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S ri... 129 1e-30
At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S ri... 127 3e-30
At5g01850.1 68418.m00104 protein kinase, putative similar to pro... 30 0.73
At1g43730.1 68414.m05028 hypothetical protein 28 2.9
At5g41310.1 68418.m05020 kinesin motor protein-related 27 5.1
At5g28615.1 68418.m03493 hypothetical protein 27 5.1
At3g15880.2 68416.m02009 WD-40 repeat family protein contains Pf... 27 5.1
At3g15880.1 68416.m02008 WD-40 repeat family protein contains Pf... 27 5.1
At1g60720.1 68414.m06835 hypothetical protein 27 6.8
At5g09720.1 68418.m01126 magnesium transporter CorA-like family ... 27 9.0
At3g62390.1 68416.m07008 expressed protein several hypothetical ... 27 9.0
At3g03290.1 68416.m00326 universal stress protein (USP) family p... 27 9.0
At2g40475.1 68415.m04995 expressed protein 27 9.0
At1g02020.2 68414.m00122 nitroreductase family protein contains ... 27 9.0
At1g02020.1 68414.m00121 nitroreductase family protein contains ... 27 9.0
>At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S
ribosomal protein S9, Chlamydomonas sp., EMBL:AU066528
Length = 198
Score = 129 bits (311), Expect = 1e-30
Identities = 61/87 (70%), Positives = 74/87 (85%), Gaps = 4/87 (4%)
Frame = -2
Query: 254 QTQVFKAGLAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRP 75
QT VFK+G+AKSIHH+R+LIRQRHIRV KQ+VNIPSF+VRLDS KHIDF+L SPFGGGRP
Sbjct: 112 QTIVFKSGMAKSIHHSRVLIRQRHIRVGKQLVNIPSFMVRLDSQKHIDFALTSPFGGGRP 171
Query: 74 GRVKRKN----LRKGQGGGAANDEEED 6
GRVKR+N +K GGG A+ ++E+
Sbjct: 172 GRVKRRNEKSASKKASGGGDADGDDEE 198
Score = 68.9 bits (161), Expect = 2e-12
Identities = 31/47 (65%), Positives = 39/47 (82%)
Frame = -3
Query: 391 EKDPKRLFKGNALLRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLR 251
EK P+R+F+G ALLRR+ R G+LDE Q KLDYVL L +E+FLERRL+
Sbjct: 66 EKSPRRIFEGEALLRRMNRYGLLDESQNKLDYVLALTVENFLERRLQ 112
Score = 38.3 bits (85), Expect = 0.003
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = -1
Query: 477 GLXNKRXVWRVKYTLAGIRKAAREXXXXXXXTPR 376
GL NKR +WRV+Y+L+ IR AAR+ +PR
Sbjct: 37 GLRNKRELWRVQYSLSRIRNAARDLLTLDEKSPR 70
>At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S
ribosomal protein S9 - Chlamydomonas sp.,EMBL:AU066528
Length = 197
Score = 127 bits (307), Expect = 3e-30
Identities = 58/83 (69%), Positives = 70/83 (84%)
Frame = -2
Query: 254 QTQVFKAGLAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRP 75
QT VFK+G+AKSIHHAR+LIRQRHIRV +Q+VNIPSF+VR++S KH+DFSL SPFGGGRP
Sbjct: 112 QTIVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFMVRVESQKHVDFSLTSPFGGGRP 171
Query: 74 GRVKRKNLRKGQGGGAANDEEED 6
GRVKR+N R G + D +ED
Sbjct: 172 GRVKRRNERAGAKKASGGDGDED 194
Score = 68.9 bits (161), Expect = 2e-12
Identities = 31/47 (65%), Positives = 40/47 (85%)
Frame = -3
Query: 391 EKDPKRLFKGNALLRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLR 251
EK+P+R+F+G ALLRR+ R G+LDE Q KLDYVL L +E+FLERRL+
Sbjct: 66 EKNPRRIFEGEALLRRMNRYGLLDETQNKLDYVLALTVENFLERRLQ 112
Score = 37.5 bits (83), Expect = 0.005
Identities = 18/34 (52%), Positives = 21/34 (61%)
Frame = -1
Query: 477 GLXNKRXVWRVKYTLAGIRKAAREXXXXXXXTPR 376
GL KR +WRV+YTL+ IR AARE PR
Sbjct: 37 GLRCKRELWRVQYTLSRIRNAARELLTLDEKNPR 70
>At5g01850.1 68418.m00104 protein kinase, putative similar to
protein kinase [Arabidopsis thaliana]
gi|1054633|emb|CAA63387; contains protein kinase domain,
Pfam:PF00069
Length = 333
Score = 30.3 bits (65), Expect = 0.73
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3
Query: 81 TSTEWRFQREVNVLARVQAHN 143
+S E RF REVN+++RVQ HN
Sbjct: 57 SSLESRFVREVNMMSRVQHHN 77
>At1g43730.1 68414.m05028 hypothetical protein
Length = 320
Score = 28.3 bits (60), Expect = 2.9
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +2
Query: 197 SKFWHDGWTSPGQL 238
+KFWHD WT G L
Sbjct: 77 AKFWHDNWTGHGPL 90
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 27.5 bits (58), Expect = 5.1
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = -3
Query: 253 RRRCSKLAWRSPSIMPEF*SGKGIFVSASKL*TSHH 146
RRR S A S + F G F+ AS++ TSHH
Sbjct: 141 RRRWSLPADHSKGVDSNFNDGGSQFIEASEINTSHH 176
>At5g28615.1 68418.m03493 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 5.1
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +2
Query: 197 SKFWHDGWTSPGQL 238
+KFWHD WT G L
Sbjct: 9 AKFWHDDWTGLGPL 22
>At3g15880.2 68416.m02009 WD-40 repeat family protein contains Pfam
profile: PF00400 WD domain, G-beta repeat (7 copies)
Length = 1135
Score = 27.5 bits (58), Expect = 5.1
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = -2
Query: 308 ETRLCAWSED*GLLGASSQTQVFKAGLAKSIHHARILIRQRHIRV 174
+++LC WS D AS Q Q+ + H R+ Q I V
Sbjct: 950 DSQLCVWSMDGWEKQASKQIQIPSGHSPNPLAHTRVQFHQDQIHV 994
>At3g15880.1 68416.m02008 WD-40 repeat family protein contains Pfam
profile: PF00400 WD domain, G-beta repeat (7 copies)
Length = 1137
Score = 27.5 bits (58), Expect = 5.1
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = -2
Query: 308 ETRLCAWSED*GLLGASSQTQVFKAGLAKSIHHARILIRQRHIRV 174
+++LC WS D AS Q Q+ + H R+ Q I V
Sbjct: 950 DSQLCVWSMDGWEKQASKQIQIPSGHSPNPLAHTRVQFHQDQIHV 994
>At1g60720.1 68414.m06835 hypothetical protein
Length = 289
Score = 27.1 bits (57), Expect = 6.8
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +2
Query: 203 FWHDGWTSPGQL 238
FWHD WTS G L
Sbjct: 25 FWHDSWTSLGPL 36
>At5g09720.1 68418.m01126 magnesium transporter CorA-like family
protein (MRS2-8) contains Pfam profile PF01544:
CorA-like Mg2+ transporter protein
Length = 397
Score = 26.6 bits (56), Expect = 9.0
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -2
Query: 134 LDSGKHIDFSLKSPFGGGRPGRVKRKNLRKGQ-GGGAANDEEE 9
+DSG+HI++ SP G + R K +R G ND EE
Sbjct: 267 IDSGEHINWYPTSPTIGAKISRAKSHLVRSATVRGDDKNDVEE 309
>At3g62390.1 68416.m07008 expressed protein several hypothetical
proteins - Arabidopsis thaliana
Length = 475
Score = 26.6 bits (56), Expect = 9.0
Identities = 13/52 (25%), Positives = 25/52 (48%)
Frame = -1
Query: 282 RLRTSWSVVSDAGVQSWPGEVHPSCQNFDPAKAYSCPQASCEHPIIYCAPGL 127
++RT ++++ +G+ + + HPS P S C H +C PG+
Sbjct: 411 QMRTPVTLLNVSGLSQYRIDAHPSIYGTKPENRRSRAVQDCSH---WCLPGV 459
>At3g03290.1 68416.m00326 universal stress protein (USP) family
protein contains Pfam profile: PF00582 universal stress
protein family
Length = 274
Score = 26.6 bits (56), Expect = 9.0
Identities = 14/75 (18%), Positives = 33/75 (44%)
Frame = -2
Query: 230 LAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRPGRVKRKNL 51
+A+S+ + + + RH+R+R + ++ + I+F + G +
Sbjct: 1 MARSLKKS-VKLSLRHVRIRSSTIGFKPDSSSIEIDQRIEFLGEEETDGDSTSEYEEFEE 59
Query: 50 RKGQGGGAANDEEED 6
K +GG +E++D
Sbjct: 60 EKERGGEGEGEEQDD 74
>At2g40475.1 68415.m04995 expressed protein
Length = 193
Score = 26.6 bits (56), Expect = 9.0
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = -1
Query: 270 SWSVVSDAGVQSWPGEVHPSCQNFDPAKAYSCPQA 166
SWS S + S+ PS P K YSC ++
Sbjct: 107 SWSSASSSSSSSYSSSSPPSKVEHRPRKCYSCSRS 141
>At1g02020.2 68414.m00122 nitroreductase family protein contains
Pfam PF00881: nitroreductase family protein profile;
contains Prosite PS00343: Gram-positive cocci surface
proteins 'anchoring' hexapeptide
Length = 543
Score = 26.6 bits (56), Expect = 9.0
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 246 GVQSWPGEVHPSCQNFDPAKAY 181
G +WP V+PS N P +AY
Sbjct: 164 GSSTWPLRVNPSSGNLHPTEAY 185
>At1g02020.1 68414.m00121 nitroreductase family protein contains
Pfam PF00881: nitroreductase family protein profile;
contains Prosite PS00343: Gram-positive cocci surface
proteins 'anchoring' hexapeptide
Length = 642
Score = 26.6 bits (56), Expect = 9.0
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 246 GVQSWPGEVHPSCQNFDPAKAY 181
G +WP V+PS N P +AY
Sbjct: 164 GSSTWPLRVNPSSGNLHPTEAY 185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,066,198
Number of Sequences: 28952
Number of extensions: 209427
Number of successful extensions: 585
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 582
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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