BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0611.Seq (493 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g14310.2 68417.m02205 peroxisomal membrane protein-related co... 29 1.7 At4g14310.1 68417.m02204 peroxisomal membrane protein-related co... 29 1.7 At2g40070.1 68415.m04923 expressed protein 29 2.2 At1g18700.1 68414.m02333 DNAJ heat shock N-terminal domain-conta... 28 3.0 At5g52330.1 68418.m06494 meprin and TRAF homology domain-contain... 28 3.9 At4g04400.1 68417.m00634 hypothetical protein contains Pfam prof... 27 5.2 At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2... 27 5.2 At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 27 9.1 At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 27 9.1 At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing ... 27 9.1 At2g21450.1 68415.m02552 SNF2 domain-containing protein / helica... 27 9.1 >At4g14310.2 68417.m02205 peroxisomal membrane protein-related contains weak similarity to Peroxisomal membrane protein 2 (22 kDa peroxisomal membrane protein) (Swiss-Prot:P42925) [Mus musculus] Length = 965 Score = 29.1 bits (62), Expect = 1.7 Identities = 18/45 (40%), Positives = 24/45 (53%) Frame = +3 Query: 261 TEQPSSINHASKDIVLTSEAELANQERSREQPNFSIDQKNTTELE 395 TE+PS N SKD+ LTS L R+ E D++N E+E Sbjct: 460 TEEPSKENDVSKDVNLTS--NLTEILRANEALEEIDDEENREEME 502 >At4g14310.1 68417.m02204 peroxisomal membrane protein-related contains weak similarity to Peroxisomal membrane protein 2 (22 kDa peroxisomal membrane protein) (Swiss-Prot:P42925) [Mus musculus] Length = 1087 Score = 29.1 bits (62), Expect = 1.7 Identities = 18/45 (40%), Positives = 24/45 (53%) Frame = +3 Query: 261 TEQPSSINHASKDIVLTSEAELANQERSREQPNFSIDQKNTTELE 395 TE+PS N SKD+ LTS L R+ E D++N E+E Sbjct: 460 TEEPSKENDVSKDVNLTS--NLTEILRANEALEEIDDEENREEME 502 >At2g40070.1 68415.m04923 expressed protein Length = 607 Score = 28.7 bits (61), Expect = 2.2 Identities = 17/52 (32%), Positives = 27/52 (51%) Frame = +3 Query: 288 ASKDIVLTSEAELANQERSREQPNFSIDQKNTTELESPINQTETTEPQFSVS 443 A KD L+ E+ + R +EQ N ++ N E E+P+ T P F++S Sbjct: 29 AEKDEELSLFLEM--RRREKEQDNLLLNN-NPDEFETPLGSKHGTSPVFNIS 77 >At1g18700.1 68414.m02333 DNAJ heat shock N-terminal domain-containing protein low similarity to SP|Q9QYI8 DnaJ homolog subfamily B member 7 Mus musculus; contains Pfam profile PF00226 DnaJ domain Length = 700 Score = 28.3 bits (60), Expect = 3.0 Identities = 22/70 (31%), Positives = 34/70 (48%) Frame = +3 Query: 258 TTEQPSSINHASKDIVLTSEAELANQERSREQPNFSIDQKNTTELESPINQTETTEPQFS 437 +T+QP+ N S + + E +ER REQ ++N E E P + T+ EP+ + Sbjct: 636 STQQPAQTNQPSPNQPADNVEEKKKKERKREQ-----RRRNAKEEEVPASITD-NEPKDA 689 Query: 438 VSKQPSGEIS 467 V SG S Sbjct: 690 VQILSSGSDS 699 >At5g52330.1 68418.m06494 meprin and TRAF homology domain-containing protein / MATH domain-containing protein weak similarity to ubiquitin-specific protease 12 [Arabidopsis thaliana] GI:11993471; contains Pfam profile PF00917: MATH domain Length = 397 Score = 27.9 bits (59), Expect = 3.9 Identities = 14/46 (30%), Positives = 22/46 (47%) Frame = -1 Query: 373 WSMEKFGCSLDRSWFASSASDVNTMSLDA*FIDDGCSVVSHYQLVL 236 W ++KF + + FASS ++ S +GC V +H L L Sbjct: 23 WKIKKFS-QISKREFASSVFEIGGYSWHILMYPEGCDVSNHLSLFL 67 >At4g04400.1 68417.m00634 hypothetical protein contains Pfam profile PF03384: Drosophila protein of unknown function, DUF287 Length = 735 Score = 27.5 bits (58), Expect = 5.2 Identities = 16/66 (24%), Positives = 31/66 (46%) Frame = +3 Query: 261 TEQPSSINHASKDIVLTSEAELANQERSREQPNFSIDQKNTTELESPINQTETTEPQFSV 440 TE+ S HA+ D VL + + + +Q + I +++ E+ +T TTE + Sbjct: 626 TEEASQDGHATDDDVLFASPDGYVTTGNEQQRDIVISERSPEVAENSNKETNTTEKEVKG 685 Query: 441 SKQPSG 458 + +G Sbjct: 686 KAKKNG 691 >At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2 / CNBT2 (CNGC19) identical to cyclic nucleotide-binding transporter 2 (CNBT2) GI:8131900 from [Arabidopsis thaliana]; member of the cyclic nucleotide-gated channel family (CNGC)- see PMID:11500563 Length = 743 Score = 27.5 bits (58), Expect = 5.2 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +3 Query: 387 ELESPINQTETTEPQFSVSKQPSGEISAITE 479 ++ PI+ T TEP FS S Q S + S+ + Sbjct: 50 QMSGPIHSTRRTEPLFSPSPQESPDSSSTVD 80 >At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related low similarity to clathrin assembly protein AP180 [Xenopus laevis] GI:6492344; contains Pfam profile PF01417: ENTH domain Length = 591 Score = 26.6 bits (56), Expect = 9.1 Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 3/42 (7%) Frame = +3 Query: 315 EAELANQERSREQPNFSIDQKN---TTELESPINQTETTEPQ 431 E E QE+ EQP +++N TE + P+ + E EP+ Sbjct: 323 EKEEEEQEQEEEQPEEPAEEENQNENTENDQPLIEEEEEEPK 364 >At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1 protein, putative strong similarity to SP|O22315 Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis thaliana}; cDNA NCBI_gi:15810292 supports a truncated version while protein evidence supports a longer model. Length = 278 Score = 26.6 bits (56), Expect = 9.1 Identities = 25/90 (27%), Positives = 40/90 (44%), Gaps = 4/90 (4%) Frame = +2 Query: 92 FSHQ*TR-NNLENPHKQ*SPNRQQTLKKQQSNYYQ*TRNRLEKRSRP*NQNQLIMRNYRT 268 FSH+ R ++ SP+R ++ K +S +R+R RSR +++ R+ Sbjct: 183 FSHEYVRVREYDSRRDSRSPSRGRSYSKSRSRGRSPSRSRSRSRSRSKSRSPKAKSLRRS 242 Query: 269 TIVDKSRIQRHCINV*SRTSKPR---TIER 349 SR R SR+ PR T+ER Sbjct: 243 PAKSTSRSPRSRSRSKSRSLSPRGWVTVER 272 >At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing protein contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 1056 Score = 26.6 bits (56), Expect = 9.1 Identities = 19/66 (28%), Positives = 30/66 (45%), Gaps = 4/66 (6%) Frame = +3 Query: 258 TTEQPSSINHASKDIVLTSEAELANQERSRE----QPNFSIDQKNTTELESPINQTETTE 425 TT Q ++N + + A+ AN E + QP S D + TT+ E NQ + Sbjct: 969 TTNQAQNLNPSQFQAAMQPPADKANLEPQNQALRLQPMISGDGQGTTDGEVDKNQRYQST 1028 Query: 426 PQFSVS 443 QF+ + Sbjct: 1029 LQFAAN 1034 >At2g21450.1 68415.m02552 SNF2 domain-containing protein / helicase domain-containing protein low similarity to SP|Q61687 Transcriptional regulator ATRX {Mus musculus}; contains PFam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain Length = 816 Score = 26.6 bits (56), Expect = 9.1 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -1 Query: 406 LIGDSSSVVFFWSMEKFGCSLDRSWFASS 320 + GDSS+ WSME+F SL+ F S Sbjct: 671 ITGDSSNEQREWSMERFNNSLEAKVFFGS 699 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,320,889 Number of Sequences: 28952 Number of extensions: 172384 Number of successful extensions: 461 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 451 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 461 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 858708096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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