Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0608.Seq
(488 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 129 3e-31
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 99 3e-22
SPCC162.04c |wtf13||wtf element Wtf13|Schizosaccharomyces pombe|... 29 0.38
SPBC21H7.03c |||acid phosphatase |Schizosaccharomyces pombe|chr ... 28 0.66
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 0.87
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 3.5
SPCC4G3.09c |gyp3||GTPase activating protein Gyp3|Schizosaccharo... 26 3.5
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 3.5
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 25 4.6
SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomy... 25 4.6
SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 25 4.6
SPBC1539.04 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 6.1
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 25 8.1
SPBC11B10.03 |cog8||Golgi transport complex subunit Cog8 |Schizo... 25 8.1
SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |S... 25 8.1
SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch... 25 8.1
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 129 bits (311), Expect = 3e-31
Identities = 55/77 (71%), Positives = 65/77 (84%)
Frame = -2
Query: 484 TDGGLEYTFECIGNVGTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGT 305
TDGGL++TF+C GNV MR+ALEACHKGWG S++IGVAAAG+EISTRPFQLVTGR W+G
Sbjct: 263 TDGGLDWTFDCTGNVHVMRSALEACHKGWGQSIVIGVAAAGQEISTRPFQLVTGRVWRGC 322
Query: 304 AFGGYKSRESVPKLVDD 254
AFGG K R +P LV +
Sbjct: 323 AFGGVKGRSQLPDLVKE 339
Score = 49.6 bits (113), Expect = 2e-07
Identities = 21/40 (52%), Positives = 29/40 (72%)
Frame = -1
Query: 254 YLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVVDM 135
YL+ KL +D+++TH PLKEINEAF MH G I+ V+ +
Sbjct: 340 YLDHKLEIDKYITHRRPLKEINEAFTDMHNGNCIKTVLSI 379
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 99.1 bits (236), Expect = 3e-22
Identities = 42/78 (53%), Positives = 55/78 (70%)
Frame = -2
Query: 487 LTDGGLEYTFECIGNVGTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKG 308
+TDGG++Y F+C GNV M+ L+ CHKGWG +IGVAAAG+ + RPF +VTGR G
Sbjct: 258 VTDGGVDYAFDCTGNVTVMQQELQFCHKGWGKLCVIGVAAAGKTLDFRPFLVVTGRQVLG 317
Query: 307 TAFGGYKSRESVPKLVDD 254
+AFGG K R +P VD+
Sbjct: 318 SAFGGVKGRSELPNFVDE 335
Score = 49.2 bits (112), Expect = 3e-07
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = -1
Query: 254 YLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVVDM 135
Y++ +DE++T+ PLK IN+AF MH GK IR VVDM
Sbjct: 336 YMQGHFKVDEYITNEEPLKNINKAFDHMHEGKCIRCVVDM 375
>SPCC162.04c |wtf13||wtf element Wtf13|Schizosaccharomyces pombe|chr
3|||Manual
Length = 418
Score = 29.1 bits (62), Expect = 0.38
Identities = 17/53 (32%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = +3
Query: 12 LFFYCVNKMH--VVYLNYT-CSKC*RKKV*QVYKNSINIFP*KLHVNYCTDRF 161
LFFY + ++ V+Y N+T S+C +K Q+Y+ + FP ++ C + F
Sbjct: 314 LFFYEMGSLYIPVIYSNHTRTSRCSQKDA-QIYREWYSFFPRYVYSTNCMNDF 365
>SPBC21H7.03c |||acid phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 28.3 bits (60), Expect = 0.66
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +1
Query: 232 KGSFFSKYHLQAL--VHFLYFCNLQKLFPSRCDL*QV 336
+ +FF K HL L H YF L FP C++ QV
Sbjct: 27 ESNFFFKEHLGTLSPYHEPYFDGLDSAFPETCEIQQV 63
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.9 bits (59), Expect = 0.87
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 303 AVPFQVRPVTSWNGRVLISSPAAATPMIT 389
+VP+ PVTS N + S+P +TP+ T
Sbjct: 501 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 529
Score = 27.9 bits (59), Expect = 0.87
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 303 AVPFQVRPVTSWNGRVLISSPAAATPMIT 389
+VP+ PVTS N + S+P +TP+ T
Sbjct: 560 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 588
>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
Itr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = -2
Query: 397 GVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGG 293
G V+IG + G E++ + +T T G GG
Sbjct: 110 GALVVIGTSLGGHELTNGGKEFITSATSLGALLGG 144
>SPCC4G3.09c |gyp3||GTPase activating protein
Gyp3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 635
Score = 25.8 bits (54), Expect = 3.5
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -2
Query: 298 GGYKSRESVPKLVDDTWRRSCL 233
GGY+ + PKL + WR +C+
Sbjct: 315 GGYELLQRNPKLYETLWRCACI 336
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/31 (35%), Positives = 14/31 (45%), Gaps = 3/31 (9%)
Frame = -1
Query: 377 CSCCW---RRDQHSSIPTCYRSHLEGNSFWR 294
C CW + D + P C R + E N WR
Sbjct: 42 CRFCWHHIKEDLNGRCPACRRLYTEENVQWR 72
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1919
Score = 25.4 bits (53), Expect = 4.6
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 116 IYTIFVHLLNLFSSAFTTSIIKIHN 42
IYT+F HLLN++ S K N
Sbjct: 1720 IYTLFEHLLNIYKETINDSDKKEKN 1744
>SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 557
Score = 25.4 bits (53), Expect = 4.6
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -2
Query: 436 TMRAALEACHKGWGVSVIIGVA-AAGEEIS 350
++ AA E H+GW + IG A A G+ IS
Sbjct: 227 SLNAAFEHVHQGWRIMFGIGAAPALGQLIS 256
>SPAC1952.17c ||SPAC890.01c|GTPase activating
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 619
Score = 25.4 bits (53), Expect = 4.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 200 KEINEAFHLMHAGKSIRAVVDM*FLRKNIY 111
K +N F L H K+ + DM ++RKN Y
Sbjct: 444 KLLNLTFELQHLRKTSKNDEDMSYVRKNSY 473
>SPBC1539.04 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 279
Score = 25.0 bits (52), Expect = 6.1
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 248 PSIIYKLWYTFSTFVTSKSCSLPGATCNKLEWTSADLFSSSS 373
P +I W+TF T+V SK + P T S+ + SS
Sbjct: 238 PPVIKNAWHTFKTYV-SKFGASPVGTAQSRPTASSSTTAPSS 278
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 24.6 bits (51), Expect = 8.1
Identities = 8/35 (22%), Positives = 19/35 (54%)
Frame = -1
Query: 245 KKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVV 141
K+ P F+T + + ++ +HL+ G S +++
Sbjct: 1026 KEAPPSNFLTWEIIISDLTRTYHLIPRGSSTVSII 1060
>SPBC11B10.03 |cog8||Golgi transport complex subunit Cog8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 400
Score = 24.6 bits (51), Expect = 8.1
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -3
Query: 366 LEKRSALVHSNLLQVAPGREQLL 298
LEKR A +H+ L++ R+QLL
Sbjct: 48 LEKRKAELHAQKLKLVQKRQQLL 70
>SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 517
Score = 24.6 bits (51), Expect = 8.1
Identities = 17/38 (44%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = +2
Query: 272 YTFSTFV-TSKSCSLPGATCNKLEWTSADLFSSSSYTN 382
YT T+ T SCS PGA C S + S SSY N
Sbjct: 386 YTRPTYGRTDTSCSAPGAFC------STQINSPSSYIN 417
>SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 593
Score = 24.6 bits (51), Expect = 8.1
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = +3
Query: 294 PPKAVPFQVRPVTSWNGRVLISSPAAATPMITDTPH 401
PP +V ++P +++NGR + P+++ P I PH
Sbjct: 168 PPSSV--HMKP-SAFNGRKVSRRPSSSPPPIPSIPH 200
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,173,055
Number of Sequences: 5004
Number of extensions: 46713
Number of successful extensions: 133
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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