Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0603.Seq
(493 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 136 1e-32
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 127 6e-30
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 126 1e-29
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 107 4e-24
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 61 4e-10
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 49 2e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 4e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.52
SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.2
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 2.8
SB_7343| Best HMM Match : EGF (HMM E-Value=0) 28 4.8
SB_49479| Best HMM Match : R3H (HMM E-Value=0.0079) 27 8.4
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 136 bits (328), Expect = 1e-32
Identities = 79/139 (56%), Positives = 92/139 (66%), Gaps = 4/139 (2%)
Frame = +3
Query: 66 MAKAPAVGIDLGTTYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 245
MAK PAVGIDLGTTYSCVGV HGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 246 MNPTTQYSMPNVSXDVSSKMLLCKPT*STGLSR--LSVMDAXLRSRXHIXVK--TKPFSP 413
MNP + ++ + + K P + ++ V+ R + + K K F P
Sbjct: 61 MNP--ENTVFDAKRLIGRK--FDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFP 116
Query: 414 RKSXSMVLTKMNETAEXIL 470
+ SMVLTKM ETAE L
Sbjct: 117 EEISSMVLTKMKETAEAYL 135
Score = 85.8 bits (203), Expect = 2e-17
Identities = 39/81 (48%), Positives = 51/81 (62%)
Frame = +2
Query: 251 PHNTIFDAKRLXGRKFEDATVQADMKHWPFEVVSDGCKXKIKXXYXGEDKTFFPEEVXFH 430
P NT+FDAKRL GRKF+D V DM HWPF V+ +G + KI+ + GE K+FFPEE+
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 431 GAYENE*NCRXYLGKTVQNAV 493
+ + YLG V +AV
Sbjct: 123 VLTKMKETAEAYLGAKVTDAV 143
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 127 bits (306), Expect = 6e-30
Identities = 58/64 (90%), Positives = 60/64 (93%)
Frame = +3
Query: 66 MAKAPAVGIDLGTTYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 245
M KAPA+GIDLGTTYSCVGV HGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 246 MNPT 257
MNPT
Sbjct: 61 MNPT 64
Score = 81.4 bits (192), Expect = 4e-16
Identities = 36/57 (63%), Positives = 43/57 (75%)
Frame = +2
Query: 251 PHNTIFDAKRLXGRKFEDATVQADMKHWPFEVVSDGCKXKIKXXYXGEDKTFFPEEV 421
P NTIFDAKRL GR+F+D V+ DMKHW FEVV++ + K+K Y GE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEV 119
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 126 bits (304), Expect = 1e-29
Identities = 70/139 (50%), Positives = 88/139 (63%), Gaps = 2/139 (1%)
Frame = +3
Query: 66 MAKAPAVGIDLGTTYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 245
M KAPA+GIDLGTTYSCVGV +GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 246 MNPTTQYSMPNVSXDVSSKMLLCKPT*STGLSRLSVMDAXLRSRXHIXVK--TKPFSPRK 419
+NP + ++ + + + L ++ + + + K K F+ +
Sbjct: 61 LNP--ENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEE 118
Query: 420 SXSMVLTKMNETAEXILAK 476
SMVL KM ETAE L +
Sbjct: 119 ISSMVLGKMKETAEAYLGQ 137
Score = 88.6 bits (210), Expect = 2e-18
Identities = 42/81 (51%), Positives = 55/81 (67%)
Frame = +2
Query: 251 PHNTIFDAKRLXGRKFEDATVQADMKHWPFEVVSDGCKXKIKXXYXGEDKTFFPEEVXFH 430
P NTIFDAKRL GR+++DATVQ+DMK WPF+++SD K KI+ Y GE KTF EE+
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 431 GAYENE*NCRXYLGKTVQNAV 493
+ + YLG+ V +AV
Sbjct: 123 VLGKMKETAEAYLGQKVTSAV 143
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 107 bits (258), Expect = 4e-24
Identities = 68/140 (48%), Positives = 83/140 (59%), Gaps = 9/140 (6%)
Frame = +3
Query: 84 VGIDLGTTYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPTT 260
+GIDLGTTYSCVGV +G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNP-- 761
Query: 261 QYSMPNVSXDVSSKMLLCKPT*STGLSR------LSVMDAXLRSRXHIXVK--TKPFSPR 416
N D +K L+ + T + V+D + + VK K F+
Sbjct: 762 ----ENTVFD--AKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAE 815
Query: 417 KSXSMVLTKMNETAEXILAK 476
+ +MVLTKM ETAE L K
Sbjct: 816 EISAMVLTKMKETAEAYLGK 835
Score = 66.9 bits (156), Expect = 8e-12
Identities = 34/81 (41%), Positives = 47/81 (58%)
Frame = +2
Query: 251 PHNTIFDAKRLXGRKFEDATVQADMKHWPFEVVSDGCKXKIKXXYXGEDKTFFPEEVXFH 430
P NT+FDAKRL GR ++D +VQ D+K +PF+VV K ++ E KTF EE+
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 431 GAYENE*NCRXYLGKTVQNAV 493
+ + YLGK V +AV
Sbjct: 821 VLTKMKETAEAYLGKKVTHAV 841
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 61.3 bits (142), Expect = 4e-10
Identities = 28/63 (44%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +3
Query: 72 KAPAVGIDLGTTYSCVGVXX--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 245
K +GIDLGTTYSCVG G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 246 MNP 254
+NP
Sbjct: 90 INP 92
Score = 29.9 bits (64), Expect = 1.2
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 251 PHNTIFDAKRLXGRKFEDATVQADMKHWPFEVVSD 355
P NTI+DAKR G+ F + K + F V +D
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTD 126
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 49.2 bits (112), Expect = 2e-06
Identities = 22/52 (42%), Positives = 32/52 (61%)
Frame = +3
Query: 84 VGIDLGTTYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 239
VG D+G + V G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 45.6 bits (103), Expect = 2e-05
Identities = 23/56 (41%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = +2
Query: 257 NTIFDAKRLXGRKFEDATVQADMKHWPFEVVS-DGCKXKIKXXYXGEDKTFFPEEV 421
NTI KR GRKF D VQ ++ H P++VV I+ Y G+ + F PE+V
Sbjct: 121 NTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQV 176
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 4e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +3
Query: 135 GKVEIIANDQGNRTTPSYVAFTDTERLI 218
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.52
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +3
Query: 144 EIIANDQGNRTTPSYVAFT-DTERL 215
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 665
Score = 29.9 bits (64), Expect = 1.2
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +2
Query: 53 LKIQNGKSTRSRN-RSGYHVLLRWCLXXREGGDHRQRPGQQDHSVLCCVHRHRASXRRCR 229
L++ N + ++ + R GY L R C R + +R + H++L R +RC
Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282
Query: 230 QEPGGDEPHNTIFDA 274
G +P NT F+A
Sbjct: 283 GTASGMDPKNTPFNA 297
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 2.8
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 12 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 98
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_7343| Best HMM Match : EGF (HMM E-Value=0)
Length = 1233
Score = 27.9 bits (59), Expect = 4.8
Identities = 17/72 (23%), Positives = 28/72 (38%)
Frame = +3
Query: 105 TYSCVGVXXHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTTQYSMPNVS 284
T++ + H I+ N G +TP + T A + + M PT V+
Sbjct: 559 THTTTSITLHPSSAILDNSLGPASTPRTTISSTTHVSSTPIASSSMVMTPTAINPSATVT 618
Query: 285 XDVSSKMLLCKP 320
V S ++ KP
Sbjct: 619 TTVPSTSVVVKP 630
>SB_49479| Best HMM Match : R3H (HMM E-Value=0.0079)
Length = 605
Score = 27.1 bits (57), Expect = 8.4
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 198 TDTERLIGDAAKNQVAMNPT 257
TD ERL GD K+ V +NPT
Sbjct: 386 TDEERLSGDKDKDVVQVNPT 405
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,462,115
Number of Sequences: 59808
Number of extensions: 316641
Number of successful extensions: 923
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 860
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 914
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1050596726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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